Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0091 and Psyr_0092 are separated by 286 nucleotides Psyr_0092 and Psyr_0093 are separated by 45 nucleotides Psyr_0093 and Psyr_RNA1 are separated by 12 nucleotides Psyr_RNA1 and Psyr_0094 are separated by 45 nucleotides
Psyr_0091: Psyr_0091 - succinate semialdehyde dehydrogenase, at 99,948 to 101,390
_0091
Psyr_0092: Psyr_0092 - Lysine exporter protein (LYSE/YGGA), at 101,677 to 102,312
_0092
Psyr_0093: Psyr_0093 - Flavoprotein monooxygenase, at 102,358 to 103,761
_0093
Psyr_RNA1: Psyr_RNA1 - tRNA-Arg, at 103,774 to 103,850
_RNA1
Psyr_0094: Psyr_0094 - hypothetical protein, at 103,896 to 104,600
_0094
Position (kb) 102
103
104 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 101.402 kb on - strand at 101.402 kb on - strand at 101.402 kb on - strand at 101.436 kb on + strand at 101.557 kb on - strand at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.958 kb on + strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 102.301 kb on + strand at 102.302 kb on - strand at 102.302 kb on - strand at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.585 kb on + strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.882 kb on + strand, within Psyr_0093 at 102.883 kb on - strand, within Psyr_0093 at 102.883 kb on - strand, within Psyr_0093 at 102.948 kb on + strand, within Psyr_0093 at 102.948 kb on + strand, within Psyr_0093 at 102.949 kb on - strand, within Psyr_0093 at 102.949 kb on - strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 103.182 kb on - strand, within Psyr_0093 at 103.182 kb on - strand, within Psyr_0093 at 103.208 kb on + strand, within Psyr_0093 at 103.208 kb on + strand, within Psyr_0093 at 103.208 kb on + strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.524 kb on + strand, within Psyr_0093 at 103.524 kb on + strand, within Psyr_0093 at 103.524 kb on + strand, within Psyr_0093 at 103.524 kb on + strand, within Psyr_0093 at 103.525 kb on - strand, within Psyr_0093 at 103.525 kb on - strand, within Psyr_0093 at 103.525 kb on - strand, within Psyr_0093 at 103.569 kb on + strand, within Psyr_0093 at 103.569 kb on + strand, within Psyr_0093 at 103.570 kb on - strand, within Psyr_0093 at 103.570 kb on - strand, within Psyr_0093 at 103.605 kb on + strand, within Psyr_0093 at 103.605 kb on + strand, within Psyr_0093 at 103.756 kb on + strand at 103.756 kb on + strand at 103.851 kb on + strand at 103.851 kb on + strand at 103.851 kb on + strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.867 kb on + strand at 103.868 kb on - strand at 103.868 kb on - strand at 103.890 kb on + strand at 103.890 kb on + strand at 103.891 kb on - strand at 103.940 kb on + strand at 103.940 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 104.019 kb on + strand, within Psyr_0094 at 104.019 kb on + strand, within Psyr_0094 at 104.019 kb on + strand, within Psyr_0094 at 104.020 kb on - strand, within Psyr_0094 at 104.020 kb on - strand, within Psyr_0094 at 104.020 kb on - strand, within Psyr_0094 at 104.072 kb on - strand, within Psyr_0094 at 104.072 kb on - strand, within Psyr_0094 at 104.072 kb on - strand, within Psyr_0094 at 104.075 kb on + strand, within Psyr_0094 at 104.075 kb on + strand, within Psyr_0094 at 104.075 kb on + strand, within Psyr_0094 at 104.075 kb on + strand, within Psyr_0094 at 104.076 kb on - strand, within Psyr_0094 at 104.076 kb on - strand, within Psyr_0094 at 104.076 kb on - strand, within Psyr_0094 at 104.130 kb on + strand, within Psyr_0094 at 104.130 kb on + strand, within Psyr_0094 at 104.131 kb on - strand, within Psyr_0094 at 104.280 kb on + strand, within Psyr_0094 at 104.281 kb on - strand, within Psyr_0094 at 104.281 kb on - strand, within Psyr_0094 at 104.281 kb on - strand, within Psyr_0094 at 104.378 kb on + strand, within Psyr_0094 at 104.378 kb on + strand, within Psyr_0094 at 104.378 kb on + strand, within Psyr_0094 at 104.378 kb on + strand, within Psyr_0094 at 104.379 kb on - strand, within Psyr_0094 at 104.379 kb on - strand, within Psyr_0094 at 104.379 kb on - strand, within Psyr_0094 at 104.401 kb on + strand, within Psyr_0094 at 104.431 kb on + strand, within Psyr_0094 at 104.432 kb on - strand, within Psyr_0094 at 104.439 kb on + strand, within Psyr_0094 at 104.439 kb on + strand, within Psyr_0094 at 104.440 kb on - strand, within Psyr_0094 at 104.473 kb on + strand, within Psyr_0094 at 104.473 kb on + strand, within Psyr_0094 at 104.473 kb on + strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.562 kb on + strand at 104.573 kb on + strand at 104.573 kb on + strand at 104.573 kb on + strand at 104.574 kb on - strand at 104.574 kb on - strand at 104.574 kb on - strand at 104.574 kb on - strand at 104.574 kb on - strand at 104.665 kb on - strand at 104.684 kb on + strand at 104.684 kb on + strand at 104.685 kb on - strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.717 kb on - strand at 104.717 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 101,402 - -0.3 101,402 - -2.2 101,402 - +4.4 101,436 + -1.1 101,557 - +0.2 101,810 + Psyr_0092 0.21 +2.9 101,810 + Psyr_0092 0.21 +4.6 101,810 + Psyr_0092 0.21 -0.2 101,810 + Psyr_0092 0.21 -3.1 101,810 + Psyr_0092 0.21 -2.9 101,810 + Psyr_0092 0.21 -0.4 101,811 - Psyr_0092 0.21 -1.4 101,811 - Psyr_0092 0.21 +2.9 101,811 - Psyr_0092 0.21 -0.9 101,811 - Psyr_0092 0.21 -0.8 101,811 - Psyr_0092 0.21 -1.1 101,811 - Psyr_0092 0.21 +0.8 101,958 + Psyr_0092 0.44 +2.2 101,959 - Psyr_0092 0.44 -1.4 101,959 - Psyr_0092 0.44 +3.4 101,959 - Psyr_0092 0.44 -0.4 101,959 - Psyr_0092 0.44 -1.7 102,301 + -1.5 102,302 - -3.5 102,302 - +2.5 102,554 + Psyr_0093 0.14 +1.9 102,554 + Psyr_0093 0.14 +2.9 102,554 + Psyr_0093 0.14 +2.2 102,554 + Psyr_0093 0.14 -0.2 102,554 + Psyr_0093 0.14 -1.2 102,554 + Psyr_0093 0.14 -1.8 102,554 + Psyr_0093 0.14 -0.4 102,554 + Psyr_0093 0.14 -0.1 102,554 + Psyr_0093 0.14 -2.4 102,554 + Psyr_0093 0.14 +1.5 102,554 + Psyr_0093 0.14 -2.5 102,554 + Psyr_0093 0.14 -3.0 102,554 + Psyr_0093 0.14 +0.9 102,555 - Psyr_0093 0.14 +0.3 102,555 - Psyr_0093 0.14 -0.7 102,555 - Psyr_0093 0.14 +0.3 102,555 - Psyr_0093 0.14 -0.7 102,555 - Psyr_0093 0.14 +0.9 102,555 - Psyr_0093 0.14 +4.8 102,555 - Psyr_0093 0.14 -0.4 102,555 - Psyr_0093 0.14 +1.2 102,555 - Psyr_0093 0.14 -1.8 102,555 - Psyr_0093 0.14 -1.2 102,555 - Psyr_0093 0.14 -0.3 102,585 + Psyr_0093 0.16 -1.1 102,618 - Psyr_0093 0.19 +2.5 102,618 - Psyr_0093 0.19 +3.4 102,618 - Psyr_0093 0.19 -1.3 102,618 - Psyr_0093 0.19 +2.9 102,618 - Psyr_0093 0.19 -2.0 102,618 - Psyr_0093 0.19 -0.2 102,882 + Psyr_0093 0.37 -1.3 102,883 - Psyr_0093 0.37 -0.6 102,883 - Psyr_0093 0.37 +2.9 102,948 + Psyr_0093 0.42 -0.3 102,948 + Psyr_0093 0.42 -0.1 102,949 - Psyr_0093 0.42 +1.5 102,949 - Psyr_0093 0.42 +0.2 102,950 + Psyr_0093 0.42 -3.3 102,950 + Psyr_0093 0.42 +2.9 102,950 + Psyr_0093 0.42 +3.5 102,950 + Psyr_0093 0.42 -1.2 102,950 + Psyr_0093 0.42 -3.1 102,950 + Psyr_0093 0.42 +0.8 102,950 + Psyr_0093 0.42 -0.9 102,951 - Psyr_0093 0.42 +0.9 102,951 - Psyr_0093 0.42 -1.7 102,951 - Psyr_0093 0.42 +2.2 102,951 - Psyr_0093 0.42 +2.7 102,951 - Psyr_0093 0.42 +3.4 102,951 - Psyr_0093 0.42 +0.1 102,951 - Psyr_0093 0.42 -0.9 103,182 - Psyr_0093 0.59 +4.2 103,182 - Psyr_0093 0.59 -2.2 103,208 + Psyr_0093 0.61 -1.7 103,208 + Psyr_0093 0.61 -0.1 103,208 + Psyr_0093 0.61 -1.8 103,209 - Psyr_0093 0.61 +2.7 103,209 - Psyr_0093 0.61 -2.5 103,209 - Psyr_0093 0.61 +2.2 103,209 - Psyr_0093 0.61 +3.8 103,209 - Psyr_0093 0.61 -1.1 103,209 - Psyr_0093 0.61 +5.6 103,209 - Psyr_0093 0.61 +3.1 103,209 - Psyr_0093 0.61 -1.0 103,524 + Psyr_0093 0.83 +4.2 103,524 + Psyr_0093 0.83 +0.3 103,524 + Psyr_0093 0.83 -2.6 103,524 + Psyr_0093 0.83 +2.7 103,525 - Psyr_0093 0.83 +1.5 103,525 - Psyr_0093 0.83 -0.4 103,525 - Psyr_0093 0.83 -3.2 103,569 + Psyr_0093 0.86 +3.8 103,569 + Psyr_0093 0.86 -1.4 103,570 - Psyr_0093 0.86 +0.0 103,570 - Psyr_0093 0.86 +1.2 103,605 + Psyr_0093 0.89 -1.4 103,605 + Psyr_0093 0.89 -1.4 103,756 + -1.7 103,756 + +4.2 103,851 + +3.2 103,851 + -1.1 103,851 + -1.7 103,852 - -0.8 103,852 - +3.4 103,852 - +2.7 103,852 - -1.5 103,852 - -3.9 103,852 - -2.0 103,867 + +1.5 103,868 - -0.3 103,868 - +1.5 103,890 + +1.0 103,890 + +3.1 103,891 - +3.5 103,940 + -3.2 103,940 + -2.0 103,955 + -1.2 103,955 + -0.1 103,955 + +3.2 103,955 + -0.9 103,955 + +2.5 103,955 + +2.2 103,955 + +4.3 103,955 + +2.6 103,955 + -1.1 103,955 + -0.8 103,955 + -0.2 103,955 + -0.7 103,955 + -0.7 103,955 + -0.5 103,956 - +3.4 103,956 - -1.2 103,956 - +1.5 103,956 - +0.0 103,956 - +1.5 103,956 - -0.6 103,956 - -1.9 103,956 - -2.2 103,956 - -0.8 103,956 - +1.9 103,956 - +1.9 103,956 - +3.8 103,956 - -1.1 103,956 - +4.3 103,956 - -3.0 104,019 + Psyr_0094 0.17 -5.0 104,019 + Psyr_0094 0.17 -0.3 104,019 + Psyr_0094 0.17 +3.6 104,020 - Psyr_0094 0.18 +1.5 104,020 - Psyr_0094 0.18 -1.1 104,020 - Psyr_0094 0.18 +2.9 104,072 - Psyr_0094 0.25 +0.8 104,072 - Psyr_0094 0.25 +4.0 104,072 - Psyr_0094 0.25 -0.1 104,075 + Psyr_0094 0.25 -2.9 104,075 + Psyr_0094 0.25 -3.5 104,075 + Psyr_0094 0.25 -0.2 104,075 + Psyr_0094 0.25 +2.2 104,076 - Psyr_0094 0.26 -0.1 104,076 - Psyr_0094 0.26 +2.2 104,076 - Psyr_0094 0.26 -1.5 104,130 + Psyr_0094 0.33 -1.5 104,130 + Psyr_0094 0.33 +1.2 104,131 - Psyr_0094 0.33 -0.6 104,280 + Psyr_0094 0.54 +3.4 104,281 - Psyr_0094 0.55 +4.6 104,281 - Psyr_0094 0.55 -4.4 104,281 - Psyr_0094 0.55 +3.4 104,378 + Psyr_0094 0.68 +1.1 104,378 + Psyr_0094 0.68 -0.4 104,378 + Psyr_0094 0.68 +4.2 104,378 + Psyr_0094 0.68 -0.2 104,379 - Psyr_0094 0.69 +0.1 104,379 - Psyr_0094 0.69 -0.3 104,379 - Psyr_0094 0.69 +2.7 104,401 + Psyr_0094 0.72 -1.1 104,431 + Psyr_0094 0.76 +4.8 104,432 - Psyr_0094 0.76 -2.5 104,439 + Psyr_0094 0.77 -1.7 104,439 + Psyr_0094 0.77 -3.7 104,440 - Psyr_0094 0.77 +0.2 104,473 + Psyr_0094 0.82 -0.6 104,473 + Psyr_0094 0.82 +2.7 104,473 + Psyr_0094 0.82 +1.0 104,474 - Psyr_0094 0.82 +3.8 104,474 - Psyr_0094 0.82 -2.1 104,474 - Psyr_0094 0.82 -1.6 104,474 - Psyr_0094 0.82 +2.5 104,474 - Psyr_0094 0.82 +0.9 104,562 + -1.6 104,573 + +0.3 104,573 + +1.0 104,573 + +4.6 104,574 - -4.2 104,574 - +0.9 104,574 - -3.2 104,574 - -1.5 104,574 - -3.8 104,665 - -1.8 104,684 + -0.3 104,684 + -0.1 104,685 - +3.4 104,716 + -4.7 104,716 + +3.1 104,716 + +0.2 104,716 + -0.8 104,716 + -4.8 104,716 + -0.8 104,716 + -1.3 104,717 - -3.5 104,717 - +0.4
Or see this region's nucleotide sequence