Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0059 and Psyr_0060 overlap by 4 nucleotides Psyr_0060 and Psyr_0061 overlap by 1 nucleotides Psyr_0061 and Psyr_0062 overlap by 4 nucleotides
Psyr_0059: Psyr_0059 - HemY, N-terminal:HemY, N-terminal:HemY, N-terminal, at 58,285 to 59,529
_0059
Psyr_0060: Psyr_0060 - Protein of unknown function DUF513, at 59,526 to 60,701
_0060
Psyr_0061: Psyr_0061 - uroporphyrinogen-III synthase, at 60,701 to 61,474
_0061
Psyr_0062: Psyr_0062 - hydroxymethylbilane synthase, at 61,471 to 62,412
_0062
Position (kb)
59
60
61 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 58.601 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.625 kb on + strand, within Psyr_0059 at 58.700 kb on + strand, within Psyr_0059 at 58.938 kb on - strand, within Psyr_0059 at 58.938 kb on - strand, within Psyr_0059 at 59.165 kb on + strand, within Psyr_0059 at 59.165 kb on + strand, within Psyr_0059 at 59.165 kb on + strand, within Psyr_0059 at 59.165 kb on + strand, within Psyr_0059 at 59.165 kb on + strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.166 kb on - strand, within Psyr_0059 at 59.169 kb on - strand, within Psyr_0059 at 59.169 kb on - strand, within Psyr_0059 at 59.169 kb on - strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.269 kb on + strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.270 kb on - strand, within Psyr_0059 at 59.448 kb on - strand at 59.506 kb on + strand at 59.506 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.516 kb on + strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.517 kb on - strand at 59.587 kb on + strand at 59.588 kb on - strand at 59.887 kb on + strand, within Psyr_0060 at 59.888 kb on - strand, within Psyr_0060 at 59.888 kb on - strand, within Psyr_0060 at 59.888 kb on - strand, within Psyr_0060 at 60.003 kb on + strand, within Psyr_0060 at 60.003 kb on + strand, within Psyr_0060 at 60.003 kb on + strand, within Psyr_0060 at 60.003 kb on + strand, within Psyr_0060 at 60.004 kb on - strand, within Psyr_0060 at 60.004 kb on - strand, within Psyr_0060 at 60.004 kb on - strand, within Psyr_0060 at 60.372 kb on - strand, within Psyr_0060 at 60.372 kb on - strand, within Psyr_0060 at 60.627 kb on - strand at 60.650 kb on - strand at 60.650 kb on - strand at 60.673 kb on + strand at 60.677 kb on - strand at 60.677 kb on - strand at 60.682 kb on - strand at 60.682 kb on - strand at 60.702 kb on + strand at 61.494 kb on - strand at 61.494 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 58,601 + Psyr_0059 0.25 -1.1 58,625 + Psyr_0059 0.27 -1.5 58,625 + Psyr_0059 0.27 -2.0 58,625 + Psyr_0059 0.27 +0.8 58,625 + Psyr_0059 0.27 -4.1 58,625 + Psyr_0059 0.27 +3.7 58,700 + Psyr_0059 0.33 +0.9 58,938 - Psyr_0059 0.52 +4.4 58,938 - Psyr_0059 0.52 -0.9 59,165 + Psyr_0059 0.71 -0.5 59,165 + Psyr_0059 0.71 -3.4 59,165 + Psyr_0059 0.71 -1.8 59,165 + Psyr_0059 0.71 -0.1 59,165 + Psyr_0059 0.71 -0.2 59,166 - Psyr_0059 0.71 +2.5 59,166 - Psyr_0059 0.71 -2.4 59,166 - Psyr_0059 0.71 -3.1 59,166 - Psyr_0059 0.71 -0.1 59,166 - Psyr_0059 0.71 +0.9 59,166 - Psyr_0059 0.71 -0.6 59,169 - Psyr_0059 0.71 -0.3 59,169 - Psyr_0059 0.71 +0.2 59,169 - Psyr_0059 0.71 -0.5 59,269 + Psyr_0059 0.79 -2.1 59,269 + Psyr_0059 0.79 -0.7 59,269 + Psyr_0059 0.79 -1.7 59,269 + Psyr_0059 0.79 +0.4 59,269 + Psyr_0059 0.79 -0.1 59,269 + Psyr_0059 0.79 -0.6 59,269 + Psyr_0059 0.79 -3.2 59,269 + Psyr_0059 0.79 +3.8 59,270 - Psyr_0059 0.79 +1.9 59,270 - Psyr_0059 0.79 -2.4 59,270 - Psyr_0059 0.79 -1.2 59,270 - Psyr_0059 0.79 -0.1 59,270 - Psyr_0059 0.79 -1.9 59,270 - Psyr_0059 0.79 +3.6 59,270 - Psyr_0059 0.79 +2.7 59,270 - Psyr_0059 0.79 +3.7 59,270 - Psyr_0059 0.79 +0.7 59,270 - Psyr_0059 0.79 +1.9 59,270 - Psyr_0059 0.79 -1.7 59,448 - -0.6 59,506 + +1.5 59,506 + +3.1 59,516 + +0.6 59,516 + -1.6 59,516 + -1.2 59,516 + -0.5 59,516 + -0.1 59,516 + +2.2 59,516 + +0.5 59,516 + +4.3 59,516 + +0.0 59,516 + -4.9 59,516 + -1.1 59,516 + +1.7 59,516 + +1.9 59,516 + -0.8 59,516 + -0.8 59,517 - -2.2 59,517 - +2.5 59,517 - -2.8 59,517 - -5.4 59,517 - -2.0 59,517 - -1.3 59,517 - -5.4 59,517 - +1.4 59,517 - +2.9 59,517 - -1.4 59,517 - -1.1 59,517 - +0.9 59,517 - -0.8 59,517 - -0.1 59,587 + +2.9 59,588 - -1.5 59,887 + Psyr_0060 0.31 +2.5 59,888 - Psyr_0060 0.31 -1.3 59,888 - Psyr_0060 0.31 +0.2 59,888 - Psyr_0060 0.31 +2.2 60,003 + Psyr_0060 0.41 -3.4 60,003 + Psyr_0060 0.41 -2.0 60,003 + Psyr_0060 0.41 -0.8 60,003 + Psyr_0060 0.41 +2.5 60,004 - Psyr_0060 0.41 +1.5 60,004 - Psyr_0060 0.41 +5.0 60,004 - Psyr_0060 0.41 -0.2 60,372 - Psyr_0060 0.72 -1.6 60,372 - Psyr_0060 0.72 -2.5 60,627 - -0.8 60,650 - +0.1 60,650 - -1.9 60,673 + +2.9 60,677 - +3.6 60,677 - +3.4 60,682 - -2.3 60,682 - -0.5 60,702 + -1.6 61,494 - -0.5 61,494 - +0.8
Or see this region's nucleotide sequence