Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0046 and Psyr_0047 are separated by 44 nucleotides Psyr_0047 and Psyr_0048 are separated by 93 nucleotides Psyr_0048 and Psyr_0049 are separated by 211 nucleotides Psyr_0049 and Psyr_0050 are separated by 156 nucleotides Psyr_0050 and Psyr_0051 are separated by 67 nucleotides
Psyr_0046: Psyr_0046 - conserved hypothetical protein, at 47,937 to 48,227
_0046
Psyr_0047: Psyr_0047 - hypothetical protein, at 48,272 to 48,574
_0047
Psyr_0048: Psyr_0048 - conserved hypothetical protein, at 48,668 to 48,898
_0048
Psyr_0049: Psyr_0049 - Cytochrome c, class I:Iron permease FTR1, at 49,110 to 51,008
_0049
Psyr_0050: Psyr_0050 - Lysine exporter protein (LYSE/YGGA), at 51,165 to 51,797
_0050
Psyr_0051: Psyr_0051 - conserved hypothetical protein, at 51,865 to 52,458
_0051
Position (kb) 49
50
51
52 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 48.175 kb on + strand, within Psyr_0046 at 48.175 kb on + strand, within Psyr_0046 at 48.175 kb on + strand, within Psyr_0046 at 48.175 kb on + strand, within Psyr_0046 at 48.176 kb on - strand, within Psyr_0046 at 48.176 kb on - strand, within Psyr_0046 at 48.176 kb on - strand, within Psyr_0046 at 48.176 kb on - strand, within Psyr_0046 at 48.226 kb on - strand at 48.235 kb on + strand at 48.235 kb on + strand at 48.235 kb on + strand at 48.235 kb on + strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.240 kb on - strand at 48.270 kb on + strand at 48.270 kb on + strand at 48.270 kb on + strand at 48.270 kb on + strand at 48.271 kb on - strand at 48.271 kb on - strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.274 kb on - strand at 48.274 kb on - strand at 48.274 kb on - strand at 48.277 kb on + strand at 48.277 kb on + strand at 48.348 kb on + strand, within Psyr_0047 at 48.348 kb on + strand, within Psyr_0047 at 48.349 kb on - strand, within Psyr_0047 at 48.368 kb on + strand, within Psyr_0047 at 48.368 kb on + strand, within Psyr_0047 at 48.368 kb on + strand, within Psyr_0047 at 48.369 kb on - strand, within Psyr_0047 at 48.369 kb on - strand, within Psyr_0047 at 48.447 kb on + strand, within Psyr_0047 at 48.447 kb on + strand, within Psyr_0047 at 48.447 kb on + strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.451 kb on + strand, within Psyr_0047 at 48.464 kb on + strand, within Psyr_0047 at 48.465 kb on - strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.469 kb on - strand, within Psyr_0047 at 48.469 kb on - strand, within Psyr_0047 at 48.469 kb on - strand, within Psyr_0047 at 48.469 kb on - strand, within Psyr_0047 at 48.512 kb on + strand, within Psyr_0047 at 48.523 kb on + strand, within Psyr_0047 at 48.568 kb on + strand at 48.568 kb on + strand at 48.569 kb on - strand at 48.592 kb on - strand at 48.635 kb on + strand at 48.635 kb on + strand at 48.635 kb on + strand at 48.636 kb on - strand at 48.636 kb on - strand at 48.636 kb on - strand at 48.636 kb on - strand at 48.669 kb on + strand at 48.834 kb on - strand, within Psyr_0048 at 48.885 kb on + strand at 48.885 kb on + strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.959 kb on + strand at 48.959 kb on + strand at 48.959 kb on + strand at 48.960 kb on - strand at 48.960 kb on - strand at 48.960 kb on - strand at 49.026 kb on - strand at 49.029 kb on + strand at 49.045 kb on + strand at 49.046 kb on - strand at 49.052 kb on + strand at 49.052 kb on + strand at 49.052 kb on + strand at 49.052 kb on + strand at 49.053 kb on - strand at 49.053 kb on - strand at 49.053 kb on - strand at 49.058 kb on + strand at 49.058 kb on + strand at 49.058 kb on + strand at 49.059 kb on - strand at 49.059 kb on - strand at 49.059 kb on - strand at 49.059 kb on - strand at 49.159 kb on + strand at 49.159 kb on + strand at 49.159 kb on + strand at 49.208 kb on - strand at 49.270 kb on - strand at 49.489 kb on + strand, within Psyr_0049 at 49.490 kb on - strand, within Psyr_0049 at 49.490 kb on - strand, within Psyr_0049 at 49.588 kb on + strand, within Psyr_0049 at 49.588 kb on + strand, within Psyr_0049 at 49.589 kb on - strand, within Psyr_0049 at 49.589 kb on - strand, within Psyr_0049 at 49.589 kb on - strand, within Psyr_0049 at 49.589 kb on - strand, within Psyr_0049 at 49.717 kb on + strand, within Psyr_0049 at 49.717 kb on + strand, within Psyr_0049 at 49.717 kb on + strand, within Psyr_0049 at 49.717 kb on + strand, within Psyr_0049 at 49.717 kb on + strand, within Psyr_0049 at 49.717 kb on + strand, within Psyr_0049 at 49.718 kb on - strand, within Psyr_0049 at 49.718 kb on - strand, within Psyr_0049 at 49.718 kb on - strand, within Psyr_0049 at 49.888 kb on + strand, within Psyr_0049 at 49.888 kb on + strand, within Psyr_0049 at 49.888 kb on + strand, within Psyr_0049 at 49.888 kb on + strand, within Psyr_0049 at 49.888 kb on + strand, within Psyr_0049 at 49.889 kb on - strand, within Psyr_0049 at 49.889 kb on - strand, within Psyr_0049 at 49.889 kb on - strand, within Psyr_0049 at 49.889 kb on - strand, within Psyr_0049 at 49.889 kb on - strand, within Psyr_0049 at 49.889 kb on - strand, within Psyr_0049 at 49.889 kb on - strand, within Psyr_0049 at 50.101 kb on + strand, within Psyr_0049 at 50.101 kb on + strand, within Psyr_0049 at 50.102 kb on - strand, within Psyr_0049 at 50.122 kb on + strand, within Psyr_0049 at 50.122 kb on + strand, within Psyr_0049 at 50.123 kb on - strand, within Psyr_0049 at 50.149 kb on + strand, within Psyr_0049 at 50.150 kb on - strand, within Psyr_0049 at 50.176 kb on - strand, within Psyr_0049 at 50.185 kb on + strand, within Psyr_0049 at 50.186 kb on - strand, within Psyr_0049 at 50.186 kb on - strand, within Psyr_0049 at 50.186 kb on - strand, within Psyr_0049 at 50.186 kb on - strand, within Psyr_0049 at 50.214 kb on + strand, within Psyr_0049 at 50.214 kb on + strand, within Psyr_0049 at 50.214 kb on + strand, within Psyr_0049 at 50.214 kb on + strand, within Psyr_0049 at 50.215 kb on - strand, within Psyr_0049 at 50.215 kb on - strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.368 kb on + strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.369 kb on - strand, within Psyr_0049 at 50.377 kb on - strand, within Psyr_0049 at 50.377 kb on - strand, within Psyr_0049 at 50.458 kb on + strand, within Psyr_0049 at 50.458 kb on + strand, within Psyr_0049 at 50.459 kb on - strand, within Psyr_0049 at 50.459 kb on - strand, within Psyr_0049 at 50.459 kb on - strand, within Psyr_0049 at 50.467 kb on + strand, within Psyr_0049 at 50.468 kb on - strand at 50.591 kb on - strand, within Psyr_0049 at 50.591 kb on - strand, within Psyr_0049 at 50.830 kb on + strand at 50.831 kb on - strand at 50.890 kb on + strand at 50.977 kb on - strand at 51.010 kb on - strand at 51.010 kb on - strand at 51.063 kb on + strand at 51.063 kb on + strand at 51.063 kb on + strand at 51.063 kb on + strand at 51.064 kb on - strand at 51.064 kb on - strand at 51.100 kb on + strand at 51.101 kb on - strand at 51.299 kb on - strand, within Psyr_0050 at 51.299 kb on - strand, within Psyr_0050 at 51.434 kb on + strand, within Psyr_0050 at 51.434 kb on + strand, within Psyr_0050 at 51.456 kb on + strand, within Psyr_0050 at 51.456 kb on + strand, within Psyr_0050 at 51.457 kb on - strand, within Psyr_0050 at 51.544 kb on + strand, within Psyr_0050 at 51.544 kb on + strand, within Psyr_0050 at 51.544 kb on + strand, within Psyr_0050 at 51.553 kb on + strand, within Psyr_0050 at 51.553 kb on + strand, within Psyr_0050 at 51.554 kb on - strand, within Psyr_0050 at 51.752 kb on + strand at 51.753 kb on - strand at 51.833 kb on + strand at 51.833 kb on + strand at 51.866 kb on + strand at 51.866 kb on + strand at 51.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 48,175 + Psyr_0046 0.82 +0.1 48,175 + Psyr_0046 0.82 -2.8 48,175 + Psyr_0046 0.82 -0.8 48,175 + Psyr_0046 0.82 +3.7 48,176 - Psyr_0046 0.82 -3.8 48,176 - Psyr_0046 0.82 +1.3 48,176 - Psyr_0046 0.82 -0.1 48,176 - Psyr_0046 0.82 -1.6 48,226 - +2.2 48,235 + -1.6 48,235 + -0.2 48,235 + +2.5 48,235 + -2.0 48,236 - -3.7 48,236 - -3.3 48,236 - -0.1 48,236 - -1.1 48,236 - -1.4 48,236 - +1.4 48,240 - +2.5 48,270 + -2.3 48,270 + -2.1 48,270 + -1.9 48,270 + -0.8 48,271 - +2.0 48,271 - -0.1 48,273 + -4.0 48,273 + -2.8 48,273 + -0.1 48,273 + +1.5 48,273 + -4.1 48,273 + -3.7 48,273 + -5.6 48,274 - +0.4 48,274 - +0.9 48,274 - +2.2 48,277 + -1.4 48,277 + -0.3 48,348 + Psyr_0047 0.25 -1.1 48,348 + Psyr_0047 0.25 +2.2 48,349 - Psyr_0047 0.25 -0.5 48,368 + Psyr_0047 0.32 +1.4 48,368 + Psyr_0047 0.32 +2.9 48,368 + Psyr_0047 0.32 -0.1 48,369 - Psyr_0047 0.32 +1.9 48,369 - Psyr_0047 0.32 +0.4 48,447 + Psyr_0047 0.58 +1.5 48,447 + Psyr_0047 0.58 +2.2 48,447 + Psyr_0047 0.58 -2.9 48,448 - Psyr_0047 0.58 -2.4 48,448 - Psyr_0047 0.58 +0.7 48,448 - Psyr_0047 0.58 +0.9 48,448 - Psyr_0047 0.58 -1.4 48,448 - Psyr_0047 0.58 +2.2 48,448 - Psyr_0047 0.58 -1.1 48,448 - Psyr_0047 0.58 +1.3 48,451 + Psyr_0047 0.59 -3.1 48,464 + Psyr_0047 0.63 +2.3 48,465 - Psyr_0047 0.64 +0.6 48,468 + Psyr_0047 0.65 -1.3 48,468 + Psyr_0047 0.65 -1.4 48,468 + Psyr_0047 0.65 -2.1 48,468 + Psyr_0047 0.65 -2.5 48,468 + Psyr_0047 0.65 -0.1 48,469 - Psyr_0047 0.65 -1.9 48,469 - Psyr_0047 0.65 +1.1 48,469 - Psyr_0047 0.65 -1.1 48,469 - Psyr_0047 0.65 -2.5 48,512 + Psyr_0047 0.79 +0.9 48,523 + Psyr_0047 0.83 -3.1 48,568 + +2.5 48,568 + -3.1 48,569 - +2.2 48,592 - -0.8 48,635 + +0.9 48,635 + -1.2 48,635 + -3.1 48,636 - +0.6 48,636 - -0.9 48,636 - +2.9 48,636 - +1.0 48,669 + +0.9 48,834 - Psyr_0048 0.72 +3.6 48,885 + -0.9 48,885 + -1.4 48,886 - -2.4 48,886 - -0.5 48,886 - +0.6 48,886 - +2.2 48,886 - -0.8 48,886 - -0.3 48,959 + +1.4 48,959 + +0.9 48,959 + +4.2 48,960 - -3.1 48,960 - +0.7 48,960 - -0.3 49,026 - -1.2 49,029 + +3.8 49,045 + -0.7 49,046 - -0.1 49,052 + +0.4 49,052 + -0.6 49,052 + +4.8 49,052 + +0.8 49,053 - -2.5 49,053 - -2.6 49,053 - +1.5 49,058 + +0.0 49,058 + -0.6 49,058 + +4.6 49,059 - -0.9 49,059 - -0.8 49,059 - -0.1 49,059 - +1.5 49,159 + -0.8 49,159 + +1.6 49,159 + -5.3 49,208 - -0.2 49,270 - -1.9 49,489 + Psyr_0049 0.20 +0.9 49,490 - Psyr_0049 0.20 +1.2 49,490 - Psyr_0049 0.20 -2.1 49,588 + Psyr_0049 0.25 +3.7 49,588 + Psyr_0049 0.25 +2.5 49,589 - Psyr_0049 0.25 -0.1 49,589 - Psyr_0049 0.25 +1.8 49,589 - Psyr_0049 0.25 +1.9 49,589 - Psyr_0049 0.25 -1.7 49,717 + Psyr_0049 0.32 +0.7 49,717 + Psyr_0049 0.32 -3.4 49,717 + Psyr_0049 0.32 -1.4 49,717 + Psyr_0049 0.32 -2.4 49,717 + Psyr_0049 0.32 -0.1 49,717 + Psyr_0049 0.32 +3.4 49,718 - Psyr_0049 0.32 -1.2 49,718 - Psyr_0049 0.32 +2.8 49,718 - Psyr_0049 0.32 +2.9 49,888 + Psyr_0049 0.41 -1.4 49,888 + Psyr_0049 0.41 -2.4 49,888 + Psyr_0049 0.41 -0.5 49,888 + Psyr_0049 0.41 -0.0 49,888 + Psyr_0049 0.41 +0.6 49,889 - Psyr_0049 0.41 -0.8 49,889 - Psyr_0049 0.41 +2.6 49,889 - Psyr_0049 0.41 -0.6 49,889 - Psyr_0049 0.41 +0.4 49,889 - Psyr_0049 0.41 -0.1 49,889 - Psyr_0049 0.41 -1.8 49,889 - Psyr_0049 0.41 -0.1 50,101 + Psyr_0049 0.52 +0.6 50,101 + Psyr_0049 0.52 -1.4 50,102 - Psyr_0049 0.52 -1.5 50,122 + Psyr_0049 0.53 +3.2 50,122 + Psyr_0049 0.53 +4.2 50,123 - Psyr_0049 0.53 -0.1 50,149 + Psyr_0049 0.55 +2.2 50,150 - Psyr_0049 0.55 +1.1 50,176 - Psyr_0049 0.56 -0.9 50,185 + Psyr_0049 0.57 -2.1 50,186 - Psyr_0049 0.57 -0.4 50,186 - Psyr_0049 0.57 -3.1 50,186 - Psyr_0049 0.57 -1.8 50,186 - Psyr_0049 0.57 -0.1 50,214 + Psyr_0049 0.58 +0.1 50,214 + Psyr_0049 0.58 +0.6 50,214 + Psyr_0049 0.58 -0.7 50,214 + Psyr_0049 0.58 +0.1 50,215 - Psyr_0049 0.58 -0.7 50,215 - Psyr_0049 0.58 +0.7 50,368 + Psyr_0049 0.66 +1.9 50,368 + Psyr_0049 0.66 +1.9 50,368 + Psyr_0049 0.66 -2.1 50,368 + Psyr_0049 0.66 +3.7 50,368 + Psyr_0049 0.66 +1.9 50,368 + Psyr_0049 0.66 -1.3 50,368 + Psyr_0049 0.66 -2.7 50,368 + Psyr_0049 0.66 -0.1 50,368 + Psyr_0049 0.66 -0.5 50,368 + Psyr_0049 0.66 +1.5 50,368 + Psyr_0049 0.66 -1.1 50,368 + Psyr_0049 0.66 -2.7 50,368 + Psyr_0049 0.66 +4.2 50,368 + Psyr_0049 0.66 -2.4 50,368 + Psyr_0049 0.66 -1.0 50,368 + Psyr_0049 0.66 -0.1 50,368 + Psyr_0049 0.66 -1.7 50,368 + Psyr_0049 0.66 -3.1 50,368 + Psyr_0049 0.66 +0.2 50,368 + Psyr_0049 0.66 -2.7 50,368 + Psyr_0049 0.66 -0.1 50,368 + Psyr_0049 0.66 +0.4 50,368 + Psyr_0049 0.66 +0.1 50,369 - Psyr_0049 0.66 -0.1 50,369 - Psyr_0049 0.66 -1.1 50,369 - Psyr_0049 0.66 +0.9 50,369 - Psyr_0049 0.66 -0.1 50,369 - Psyr_0049 0.66 -0.9 50,369 - Psyr_0049 0.66 -2.6 50,369 - Psyr_0049 0.66 +2.5 50,369 - Psyr_0049 0.66 +2.7 50,369 - Psyr_0049 0.66 +0.3 50,369 - Psyr_0049 0.66 +0.3 50,369 - Psyr_0049 0.66 -2.2 50,369 - Psyr_0049 0.66 +0.6 50,369 - Psyr_0049 0.66 -2.5 50,369 - Psyr_0049 0.66 +1.5 50,369 - Psyr_0049 0.66 -1.3 50,369 - Psyr_0049 0.66 +2.9 50,369 - Psyr_0049 0.66 +1.0 50,369 - Psyr_0049 0.66 +0.6 50,369 - Psyr_0049 0.66 -1.6 50,377 - Psyr_0049 0.67 -1.1 50,377 - Psyr_0049 0.67 +2.5 50,458 + Psyr_0049 0.71 -1.9 50,458 + Psyr_0049 0.71 +2.5 50,459 - Psyr_0049 0.71 -0.1 50,459 - Psyr_0049 0.71 -1.3 50,459 - Psyr_0049 0.71 -1.1 50,467 + Psyr_0049 0.71 +3.7 50,468 - -0.1 50,591 - Psyr_0049 0.78 -1.1 50,591 - Psyr_0049 0.78 +0.9 50,830 + -1.1 50,831 - +0.3 50,890 + -0.4 50,977 - -2.2 51,010 - -0.2 51,010 - +3.1 51,063 + -0.4 51,063 + -2.7 51,063 + +3.2 51,063 + -0.8 51,064 - -2.6 51,064 - +2.2 51,100 + +0.4 51,101 - +1.1 51,299 - Psyr_0050 0.21 +3.4 51,299 - Psyr_0050 0.21 -0.7 51,434 + Psyr_0050 0.42 -0.6 51,434 + Psyr_0050 0.42 -1.3 51,456 + Psyr_0050 0.46 -2.1 51,456 + Psyr_0050 0.46 -2.4 51,457 - Psyr_0050 0.46 -1.2 51,544 + Psyr_0050 0.60 +0.4 51,544 + Psyr_0050 0.60 -0.9 51,544 + Psyr_0050 0.60 -0.6 51,553 + Psyr_0050 0.61 -3.4 51,553 + Psyr_0050 0.61 -2.0 51,554 - Psyr_0050 0.61 -0.1 51,752 + -0.1 51,753 - +0.3 51,833 + -0.8 51,833 + +3.1 51,866 + -1.7 51,866 + -3.0 51,867 - -0.3
Or see this region's nucleotide sequence