Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0045 and Psyr_0046 overlap by 4 nucleotides Psyr_0046 and Psyr_0047 are separated by 44 nucleotides Psyr_0047 and Psyr_0048 are separated by 93 nucleotides Psyr_0048 and Psyr_0049 are separated by 211 nucleotides
Psyr_0045: Psyr_0045 - oligopeptidase A, Metallo peptidase, MEROPS family M03A, at 45,889 to 47,940
_0045
Psyr_0046: Psyr_0046 - conserved hypothetical protein, at 47,937 to 48,227
_0046
Psyr_0047: Psyr_0047 - hypothetical protein, at 48,272 to 48,574
_0047
Psyr_0048: Psyr_0048 - conserved hypothetical protein, at 48,668 to 48,898
_0048
Psyr_0049: Psyr_0049 - Cytochrome c, class I:Iron permease FTR1, at 49,110 to 51,008
_0049
Position (kb) 47
48
49 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 46.960 kb on + strand, within Psyr_0045 at 46.960 kb on + strand, within Psyr_0045 at 46.960 kb on + strand, within Psyr_0045 at 46.961 kb on - strand, within Psyr_0045 at 46.961 kb on - strand, within Psyr_0045 at 46.961 kb on - strand, within Psyr_0045 at 46.961 kb on - strand, within Psyr_0045 at 47.125 kb on + strand, within Psyr_0045 at 47.125 kb on + strand, within Psyr_0045 at 47.622 kb on + strand, within Psyr_0045 at 47.623 kb on - strand, within Psyr_0045 at 47.660 kb on - strand, within Psyr_0045 at 47.660 kb on - strand, within Psyr_0045 at 47.660 kb on - strand, within Psyr_0045 at 47.660 kb on - strand, within Psyr_0045 at 47.719 kb on + strand, within Psyr_0045 at 47.719 kb on + strand, within Psyr_0045 at 47.719 kb on + strand, within Psyr_0045 at 47.720 kb on - strand, within Psyr_0045 at 47.725 kb on + strand, within Psyr_0045 at 47.725 kb on + strand, within Psyr_0045 at 47.725 kb on + strand, within Psyr_0045 at 47.725 kb on + strand, within Psyr_0045 at 47.725 kb on + strand, within Psyr_0045 at 47.725 kb on + strand, within Psyr_0045 at 47.725 kb on + strand, within Psyr_0045 at 47.726 kb on - strand, within Psyr_0045 at 47.726 kb on - strand, within Psyr_0045 at 47.726 kb on - strand, within Psyr_0045 at 47.945 kb on + strand at 47.953 kb on + strand at 47.953 kb on + strand at 48.099 kb on - strand, within Psyr_0046 at 48.175 kb on + strand, within Psyr_0046 at 48.175 kb on + strand, within Psyr_0046 at 48.175 kb on + strand, within Psyr_0046 at 48.175 kb on + strand, within Psyr_0046 at 48.176 kb on - strand, within Psyr_0046 at 48.176 kb on - strand, within Psyr_0046 at 48.176 kb on - strand, within Psyr_0046 at 48.176 kb on - strand, within Psyr_0046 at 48.226 kb on - strand at 48.235 kb on + strand at 48.235 kb on + strand at 48.235 kb on + strand at 48.235 kb on + strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.236 kb on - strand at 48.240 kb on - strand at 48.270 kb on + strand at 48.270 kb on + strand at 48.270 kb on + strand at 48.270 kb on + strand at 48.271 kb on - strand at 48.271 kb on - strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.273 kb on + strand at 48.274 kb on - strand at 48.274 kb on - strand at 48.274 kb on - strand at 48.277 kb on + strand at 48.277 kb on + strand at 48.348 kb on + strand, within Psyr_0047 at 48.348 kb on + strand, within Psyr_0047 at 48.349 kb on - strand, within Psyr_0047 at 48.368 kb on + strand, within Psyr_0047 at 48.368 kb on + strand, within Psyr_0047 at 48.368 kb on + strand, within Psyr_0047 at 48.369 kb on - strand, within Psyr_0047 at 48.369 kb on - strand, within Psyr_0047 at 48.447 kb on + strand, within Psyr_0047 at 48.447 kb on + strand, within Psyr_0047 at 48.447 kb on + strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.448 kb on - strand, within Psyr_0047 at 48.451 kb on + strand, within Psyr_0047 at 48.464 kb on + strand, within Psyr_0047 at 48.465 kb on - strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.468 kb on + strand, within Psyr_0047 at 48.469 kb on - strand, within Psyr_0047 at 48.469 kb on - strand, within Psyr_0047 at 48.469 kb on - strand, within Psyr_0047 at 48.469 kb on - strand, within Psyr_0047 at 48.512 kb on + strand, within Psyr_0047 at 48.523 kb on + strand, within Psyr_0047 at 48.568 kb on + strand at 48.568 kb on + strand at 48.569 kb on - strand at 48.592 kb on - strand at 48.635 kb on + strand at 48.635 kb on + strand at 48.635 kb on + strand at 48.636 kb on - strand at 48.636 kb on - strand at 48.636 kb on - strand at 48.636 kb on - strand at 48.669 kb on + strand at 48.834 kb on - strand, within Psyr_0048 at 48.885 kb on + strand at 48.885 kb on + strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.886 kb on - strand at 48.959 kb on + strand at 48.959 kb on + strand at 48.959 kb on + strand at 48.960 kb on - strand at 48.960 kb on - strand at 48.960 kb on - strand at 49.026 kb on - strand at 49.029 kb on + strand at 49.045 kb on + strand at 49.046 kb on - strand at 49.052 kb on + strand at 49.052 kb on + strand at 49.052 kb on + strand at 49.052 kb on + strand at 49.053 kb on - strand at 49.053 kb on - strand at 49.053 kb on - strand at 49.058 kb on + strand at 49.058 kb on + strand at 49.058 kb on + strand at 49.059 kb on - strand at 49.059 kb on - strand at 49.059 kb on - strand at 49.059 kb on - strand at 49.159 kb on + strand at 49.159 kb on + strand at 49.159 kb on + strand at 49.208 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 46,960 + Psyr_0045 0.52 +0.4 46,960 + Psyr_0045 0.52 -0.1 46,960 + Psyr_0045 0.52 +0.4 46,961 - Psyr_0045 0.52 -2.7 46,961 - Psyr_0045 0.52 +4.3 46,961 - Psyr_0045 0.52 -2.4 46,961 - Psyr_0045 0.52 +2.2 47,125 + Psyr_0045 0.60 +2.7 47,125 + Psyr_0045 0.60 +2.2 47,622 + Psyr_0045 0.84 +1.1 47,623 - Psyr_0045 0.85 +2.9 47,660 - Psyr_0045 0.86 -1.0 47,660 - Psyr_0045 0.86 -1.5 47,660 - Psyr_0045 0.86 +4.2 47,660 - Psyr_0045 0.86 +1.9 47,719 + Psyr_0045 0.89 -0.5 47,719 + Psyr_0045 0.89 -0.9 47,719 + Psyr_0045 0.89 +1.0 47,720 - Psyr_0045 0.89 -0.1 47,725 + Psyr_0045 0.89 -0.3 47,725 + Psyr_0045 0.89 -0.1 47,725 + Psyr_0045 0.89 -1.8 47,725 + Psyr_0045 0.89 +0.0 47,725 + Psyr_0045 0.89 +0.9 47,725 + Psyr_0045 0.89 -1.8 47,725 + Psyr_0045 0.89 -0.7 47,726 - Psyr_0045 0.90 -1.2 47,726 - Psyr_0045 0.90 +3.2 47,726 - Psyr_0045 0.90 -1.3 47,945 + -0.1 47,953 + +3.2 47,953 + +4.1 48,099 - Psyr_0046 0.56 -3.2 48,175 + Psyr_0046 0.82 +0.1 48,175 + Psyr_0046 0.82 -2.8 48,175 + Psyr_0046 0.82 -0.8 48,175 + Psyr_0046 0.82 +3.7 48,176 - Psyr_0046 0.82 -3.8 48,176 - Psyr_0046 0.82 +1.3 48,176 - Psyr_0046 0.82 -0.1 48,176 - Psyr_0046 0.82 -1.6 48,226 - +2.2 48,235 + -1.6 48,235 + -0.2 48,235 + +2.5 48,235 + -2.0 48,236 - -3.7 48,236 - -3.3 48,236 - -0.1 48,236 - -1.1 48,236 - -1.4 48,236 - +1.4 48,240 - +2.5 48,270 + -2.3 48,270 + -2.1 48,270 + -1.9 48,270 + -0.8 48,271 - +2.0 48,271 - -0.1 48,273 + -4.0 48,273 + -2.8 48,273 + -0.1 48,273 + +1.5 48,273 + -4.1 48,273 + -3.7 48,273 + -5.6 48,274 - +0.4 48,274 - +0.9 48,274 - +2.2 48,277 + -1.4 48,277 + -0.3 48,348 + Psyr_0047 0.25 -1.1 48,348 + Psyr_0047 0.25 +2.2 48,349 - Psyr_0047 0.25 -0.5 48,368 + Psyr_0047 0.32 +1.4 48,368 + Psyr_0047 0.32 +2.9 48,368 + Psyr_0047 0.32 -0.1 48,369 - Psyr_0047 0.32 +1.9 48,369 - Psyr_0047 0.32 +0.4 48,447 + Psyr_0047 0.58 +1.5 48,447 + Psyr_0047 0.58 +2.2 48,447 + Psyr_0047 0.58 -2.9 48,448 - Psyr_0047 0.58 -2.4 48,448 - Psyr_0047 0.58 +0.7 48,448 - Psyr_0047 0.58 +0.9 48,448 - Psyr_0047 0.58 -1.4 48,448 - Psyr_0047 0.58 +2.2 48,448 - Psyr_0047 0.58 -1.1 48,448 - Psyr_0047 0.58 +1.3 48,451 + Psyr_0047 0.59 -3.1 48,464 + Psyr_0047 0.63 +2.3 48,465 - Psyr_0047 0.64 +0.6 48,468 + Psyr_0047 0.65 -1.3 48,468 + Psyr_0047 0.65 -1.4 48,468 + Psyr_0047 0.65 -2.1 48,468 + Psyr_0047 0.65 -2.5 48,468 + Psyr_0047 0.65 -0.1 48,469 - Psyr_0047 0.65 -1.9 48,469 - Psyr_0047 0.65 +1.1 48,469 - Psyr_0047 0.65 -1.1 48,469 - Psyr_0047 0.65 -2.5 48,512 + Psyr_0047 0.79 +0.9 48,523 + Psyr_0047 0.83 -3.1 48,568 + +2.5 48,568 + -3.1 48,569 - +2.2 48,592 - -0.8 48,635 + +0.9 48,635 + -1.2 48,635 + -3.1 48,636 - +0.6 48,636 - -0.9 48,636 - +2.9 48,636 - +1.0 48,669 + +0.9 48,834 - Psyr_0048 0.72 +3.6 48,885 + -0.9 48,885 + -1.4 48,886 - -2.4 48,886 - -0.5 48,886 - +0.6 48,886 - +2.2 48,886 - -0.8 48,886 - -0.3 48,959 + +1.4 48,959 + +0.9 48,959 + +4.2 48,960 - -3.1 48,960 - +0.7 48,960 - -0.3 49,026 - -1.2 49,029 + +3.8 49,045 + -0.7 49,046 - -0.1 49,052 + +0.4 49,052 + -0.6 49,052 + +4.8 49,052 + +0.8 49,053 - -2.5 49,053 - -2.6 49,053 - +1.5 49,058 + +0.0 49,058 + -0.6 49,058 + +4.6 49,059 - -0.9 49,059 - -0.8 49,059 - -0.1 49,059 - +1.5 49,159 + -0.8 49,159 + +1.6 49,159 + -5.3 49,208 - -0.2
Or see this region's nucleotide sequence