Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0030 and Psyr_0031 are separated by 144 nucleotides Psyr_0031 and Psyr_0032 are separated by 266 nucleotides Psyr_0032 and Psyr_0033 are separated by 125 nucleotides
Psyr_0030: Psyr_0030 - transcriptional regulator, LysR family, at 34,350 to 35,300
_0030
Psyr_0031: Psyr_0031 - Alpha/beta hydrolase fold protein, at 35,445 to 36,269
_0031
Psyr_0032: Psyr_0032 - conserved hypothetical protein, at 36,536 to 37,066
_0032
Psyr_0033: Psyr_0033 - tryptophan synthase, alpha chain, at 37,192 to 38,001
_0033
Position (kb)
35
36
37 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 34.524 kb on + strand, within Psyr_0030 at 34.524 kb on + strand, within Psyr_0030 at 34.525 kb on - strand, within Psyr_0030 at 34.532 kb on + strand, within Psyr_0030 at 34.609 kb on + strand, within Psyr_0030 at 34.978 kb on + strand, within Psyr_0030 at 34.978 kb on + strand, within Psyr_0030 at 34.978 kb on + strand, within Psyr_0030 at 34.978 kb on + strand, within Psyr_0030 at 34.978 kb on + strand, within Psyr_0030 at 34.978 kb on + strand, within Psyr_0030 at 34.978 kb on + strand, within Psyr_0030 at 34.979 kb on - strand, within Psyr_0030 at 35.037 kb on + strand, within Psyr_0030 at 35.037 kb on + strand, within Psyr_0030 at 35.037 kb on + strand, within Psyr_0030 at 35.037 kb on + strand, within Psyr_0030 at 35.037 kb on + strand, within Psyr_0030 at 35.037 kb on + strand, within Psyr_0030 at 35.038 kb on - strand, within Psyr_0030 at 35.038 kb on - strand, within Psyr_0030 at 35.038 kb on - strand, within Psyr_0030 at 35.038 kb on - strand, within Psyr_0030 at 35.038 kb on - strand, within Psyr_0030 at 35.169 kb on + strand, within Psyr_0030 at 35.169 kb on + strand, within Psyr_0030 at 35.172 kb on + strand, within Psyr_0030 at 35.172 kb on + strand, within Psyr_0030 at 35.172 kb on + strand, within Psyr_0030 at 35.172 kb on + strand, within Psyr_0030 at 35.172 kb on + strand, within Psyr_0030 at 35.172 kb on + strand, within Psyr_0030 at 35.172 kb on + strand, within Psyr_0030 at 35.172 kb on + strand, within Psyr_0030 at 35.173 kb on - strand, within Psyr_0030 at 35.173 kb on - strand, within Psyr_0030 at 35.173 kb on - strand, within Psyr_0030 at 35.173 kb on - strand, within Psyr_0030 at 35.173 kb on - strand, within Psyr_0030 at 35.173 kb on - strand, within Psyr_0030 at 35.298 kb on + strand at 35.298 kb on + strand at 35.298 kb on + strand at 35.298 kb on + strand at 35.298 kb on + strand at 35.298 kb on + strand at 35.298 kb on + strand at 35.299 kb on - strand at 35.299 kb on - strand at 35.299 kb on - strand at 35.299 kb on - strand at 35.299 kb on - strand at 35.299 kb on - strand at 35.299 kb on - strand at 35.299 kb on - strand at 35.299 kb on - strand at 35.301 kb on + strand at 35.302 kb on - strand at 35.302 kb on - strand at 35.302 kb on - strand at 35.302 kb on - strand at 35.302 kb on - strand at 35.302 kb on - strand at 35.365 kb on + strand at 35.365 kb on + strand at 35.365 kb on + strand at 35.366 kb on - strand at 35.366 kb on - strand at 35.366 kb on - strand at 35.368 kb on - strand at 35.429 kb on + strand at 35.429 kb on + strand at 35.429 kb on + strand at 35.430 kb on - strand at 35.430 kb on - strand at 35.455 kb on + strand at 35.455 kb on + strand at 35.455 kb on + strand at 35.456 kb on - strand at 35.456 kb on - strand at 35.456 kb on - strand at 35.456 kb on - strand at 35.456 kb on - strand at 35.481 kb on + strand at 35.481 kb on + strand at 35.482 kb on - strand at 35.482 kb on - strand at 35.509 kb on + strand at 35.509 kb on + strand at 35.509 kb on + strand at 35.526 kb on + strand at 35.526 kb on + strand at 35.526 kb on + strand at 35.526 kb on + strand at 35.526 kb on + strand at 35.526 kb on + strand at 35.526 kb on + strand at 35.527 kb on - strand at 35.527 kb on - strand at 35.527 kb on - strand at 35.527 kb on - strand at 35.564 kb on + strand, within Psyr_0031 at 35.564 kb on + strand, within Psyr_0031 at 35.716 kb on + strand, within Psyr_0031 at 35.716 kb on + strand, within Psyr_0031 at 35.717 kb on - strand, within Psyr_0031 at 35.802 kb on + strand, within Psyr_0031 at 35.802 kb on + strand, within Psyr_0031 at 35.803 kb on - strand, within Psyr_0031 at 35.803 kb on - strand, within Psyr_0031 at 35.803 kb on - strand, within Psyr_0031 at 35.835 kb on + strand, within Psyr_0031 at 35.835 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.838 kb on + strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.839 kb on - strand, within Psyr_0031 at 35.927 kb on + strand, within Psyr_0031 at 35.949 kb on + strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 35.950 kb on - strand, within Psyr_0031 at 36.162 kb on + strand, within Psyr_0031 at 36.162 kb on + strand, within Psyr_0031 at 36.162 kb on + strand, within Psyr_0031 at 36.163 kb on - strand, within Psyr_0031 at 36.163 kb on - strand, within Psyr_0031 at 36.163 kb on - strand, within Psyr_0031 at 36.327 kb on + strand at 36.327 kb on + strand at 36.328 kb on - strand at 36.328 kb on - strand at 36.328 kb on - strand at 36.340 kb on + strand at 36.340 kb on + strand at 36.341 kb on - strand at 36.341 kb on - strand at 36.342 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.456 kb on + strand at 36.564 kb on + strand at 36.564 kb on + strand at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.645 kb on + strand, within Psyr_0032 at 36.646 kb on - strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.714 kb on - strand, within Psyr_0032 at 36.714 kb on - strand, within Psyr_0032 at 36.902 kb on - strand, within Psyr_0032 at 37.045 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.176 kb on - strand at 37.207 kb on - strand at 37.207 kb on - strand at 37.210 kb on + strand at 37.210 kb on + strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 34,524 + Psyr_0030 0.18 +1.5 34,524 + Psyr_0030 0.18 -1.6 34,525 - Psyr_0030 0.18 -2.4 34,532 + Psyr_0030 0.19 +1.1 34,609 + Psyr_0030 0.27 -1.8 34,978 + Psyr_0030 0.66 -0.1 34,978 + Psyr_0030 0.66 -0.1 34,978 + Psyr_0030 0.66 +2.2 34,978 + Psyr_0030 0.66 +0.6 34,978 + Psyr_0030 0.66 +4.3 34,978 + Psyr_0030 0.66 -0.8 34,978 + Psyr_0030 0.66 +3.4 34,979 - Psyr_0030 0.66 -1.5 35,037 + Psyr_0030 0.72 -1.8 35,037 + Psyr_0030 0.72 +3.4 35,037 + Psyr_0030 0.72 +0.5 35,037 + Psyr_0030 0.72 -0.8 35,037 + Psyr_0030 0.72 +3.2 35,037 + Psyr_0030 0.72 -1.0 35,038 - Psyr_0030 0.72 +2.7 35,038 - Psyr_0030 0.72 +0.3 35,038 - Psyr_0030 0.72 -0.1 35,038 - Psyr_0030 0.72 -5.2 35,038 - Psyr_0030 0.72 -2.9 35,169 + Psyr_0030 0.86 -1.1 35,169 + Psyr_0030 0.86 +0.6 35,172 + Psyr_0030 0.86 -1.2 35,172 + Psyr_0030 0.86 -0.4 35,172 + Psyr_0030 0.86 -2.5 35,172 + Psyr_0030 0.86 +4.0 35,172 + Psyr_0030 0.86 -1.8 35,172 + Psyr_0030 0.86 -0.1 35,172 + Psyr_0030 0.86 +0.1 35,172 + Psyr_0030 0.86 -0.3 35,173 - Psyr_0030 0.87 +0.9 35,173 - Psyr_0030 0.87 +0.5 35,173 - Psyr_0030 0.87 +0.0 35,173 - Psyr_0030 0.87 +4.0 35,173 - Psyr_0030 0.87 +4.4 35,173 - Psyr_0030 0.87 -1.1 35,298 + -0.8 35,298 + +2.2 35,298 + +3.2 35,298 + +3.4 35,298 + -0.3 35,298 + -0.8 35,298 + -0.4 35,299 - -0.1 35,299 - +4.1 35,299 - +2.2 35,299 - -1.6 35,299 - -0.4 35,299 - -1.5 35,299 - +3.1 35,299 - +0.2 35,299 - -0.1 35,301 + +0.6 35,302 - +4.6 35,302 - -0.7 35,302 - +2.5 35,302 - -0.7 35,302 - -1.8 35,302 - -1.1 35,365 + +3.5 35,365 + -3.0 35,365 + -2.5 35,366 - -0.8 35,366 - -1.9 35,366 - -0.8 35,368 - -1.7 35,429 + -1.1 35,429 + +0.9 35,429 + +3.6 35,430 - -3.3 35,430 - +1.5 35,455 + -3.2 35,455 + -2.2 35,455 + -1.0 35,456 - -1.4 35,456 - -0.4 35,456 - +0.7 35,456 - -1.5 35,456 - -2.8 35,481 + -0.8 35,481 + -1.8 35,482 - -3.7 35,482 - -1.1 35,509 + +4.1 35,509 + -1.8 35,509 + -1.2 35,526 + -1.0 35,526 + -0.7 35,526 + -0.1 35,526 + +1.9 35,526 + -0.8 35,526 + -2.5 35,526 + -1.1 35,527 - -1.8 35,527 - +3.4 35,527 - +3.9 35,527 - -0.1 35,564 + Psyr_0031 0.14 -1.0 35,564 + Psyr_0031 0.14 -2.2 35,716 + Psyr_0031 0.33 -0.8 35,716 + Psyr_0031 0.33 -0.6 35,717 - Psyr_0031 0.33 -3.4 35,802 + Psyr_0031 0.43 +4.0 35,802 + Psyr_0031 0.43 -5.2 35,803 - Psyr_0031 0.43 -0.2 35,803 - Psyr_0031 0.43 +3.5 35,803 - Psyr_0031 0.43 -5.5 35,835 + Psyr_0031 0.47 +1.5 35,835 + Psyr_0031 0.47 +0.4 35,838 + Psyr_0031 0.48 +2.0 35,838 + Psyr_0031 0.48 -0.4 35,838 + Psyr_0031 0.48 -0.1 35,838 + Psyr_0031 0.48 +0.6 35,838 + Psyr_0031 0.48 -0.6 35,838 + Psyr_0031 0.48 +1.2 35,838 + Psyr_0031 0.48 -0.1 35,838 + Psyr_0031 0.48 -1.9 35,839 - Psyr_0031 0.48 -2.4 35,839 - Psyr_0031 0.48 -0.1 35,839 - Psyr_0031 0.48 -0.9 35,839 - Psyr_0031 0.48 +1.9 35,839 - Psyr_0031 0.48 -2.2 35,839 - Psyr_0031 0.48 +0.4 35,927 + Psyr_0031 0.58 +0.2 35,949 + Psyr_0031 0.61 +0.7 35,950 - Psyr_0031 0.61 -3.4 35,950 - Psyr_0031 0.61 -0.3 35,950 - Psyr_0031 0.61 -0.1 35,950 - Psyr_0031 0.61 -0.1 35,950 - Psyr_0031 0.61 +3.1 36,162 + Psyr_0031 0.87 -0.1 36,162 + Psyr_0031 0.87 -1.8 36,162 + Psyr_0031 0.87 -0.7 36,163 - Psyr_0031 0.87 +3.4 36,163 - Psyr_0031 0.87 -0.8 36,163 - Psyr_0031 0.87 -0.6 36,327 + +2.9 36,327 + -0.5 36,328 - -2.3 36,328 - +1.6 36,328 - +3.4 36,340 + -2.5 36,340 + -0.2 36,341 - -1.3 36,341 - -3.5 36,342 + +2.7 36,454 + -1.2 36,454 + -0.1 36,454 + -2.8 36,454 + +2.7 36,454 + -1.4 36,454 + -1.0 36,454 + -1.1 36,454 + -0.6 36,454 + +0.3 36,454 + -0.2 36,454 + -3.0 36,455 - +3.6 36,455 - -3.2 36,455 - +0.1 36,455 - -0.2 36,456 + -0.2 36,564 + +0.6 36,564 + -1.7 36,633 + Psyr_0032 0.18 +1.9 36,633 + Psyr_0032 0.18 +2.7 36,633 + Psyr_0032 0.18 -0.6 36,633 + Psyr_0032 0.18 +1.9 36,634 - Psyr_0032 0.18 +1.6 36,634 - Psyr_0032 0.18 +3.7 36,634 - Psyr_0032 0.18 +1.0 36,634 - Psyr_0032 0.18 +0.6 36,634 - Psyr_0032 0.18 -0.5 36,645 + Psyr_0032 0.21 +0.7 36,646 - Psyr_0032 0.21 -0.1 36,713 + Psyr_0032 0.33 +4.6 36,713 + Psyr_0032 0.33 -0.4 36,713 + Psyr_0032 0.33 -2.2 36,714 - Psyr_0032 0.34 -0.1 36,714 - Psyr_0032 0.34 +3.6 36,902 - Psyr_0032 0.69 -2.2 37,045 + +0.7 37,140 + -3.5 37,140 + -1.8 37,140 + +4.5 37,140 + -1.7 37,140 + +0.3 37,140 + -1.9 37,140 + -2.1 37,140 + +3.4 37,140 + -0.5 37,140 + -3.8 37,140 + -0.1 37,141 - -1.9 37,141 - +0.5 37,141 - +2.7 37,141 - +1.0 37,141 - +4.7 37,141 - -0.9 37,141 - -1.7 37,141 - -0.7 37,141 - +1.8 37,141 - +1.6 37,144 + -0.3 37,144 + -0.1 37,144 + -0.1 37,144 + -2.5 37,144 + +4.6 37,144 + -0.8 37,144 + -0.9 37,145 - +0.6 37,145 - -1.1 37,145 - -0.1 37,145 - +1.3 37,145 - -0.5 37,145 - -0.6 37,145 - +1.5 37,145 - -0.5 37,145 - -0.7 37,176 - +3.9 37,207 - -0.2 37,207 - +0.5 37,210 + +1.5 37,210 + -1.1 37,211 - -0.1 37,211 - +5.0 37,211 - +0.6 37,211 - +5.4 37,211 - -0.1
Or see this region's nucleotide sequence