Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0002 and Psyr_0003 are separated by 22 nucleotides Psyr_0003 and Psyr_0004 are separated by 4 nucleotides
Psyr_0002: Psyr_0002 - DNA polymerase III, beta subunit, at 1,575 to 2,678
_0002
Psyr_0003: Psyr_0003 - DNA replication and repair protein RecF, at 2,701 to 3,804
_0003
Psyr_0004: Psyr_0004 - DNA gyrase subunit B, at 3,809 to 6,226
_0004
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2.676 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.698 kb on + strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.699 kb on - strand at 2.710 kb on - strand at 2.827 kb on - strand, within Psyr_0003 at 2.887 kb on + strand, within Psyr_0003 at 3.095 kb on + strand, within Psyr_0003 at 3.095 kb on + strand, within Psyr_0003 at 3.207 kb on + strand, within Psyr_0003 at 3.207 kb on + strand, within Psyr_0003 at 3.289 kb on + strand, within Psyr_0003 at 3.289 kb on + strand, within Psyr_0003 at 3.289 kb on + strand, within Psyr_0003 at 3.289 kb on + strand, within Psyr_0003 at 3.289 kb on + strand, within Psyr_0003 at 3.291 kb on + strand, within Psyr_0003 at 3.291 kb on + strand, within Psyr_0003 at 3.291 kb on + strand, within Psyr_0003 at 3.291 kb on + strand, within Psyr_0003 at 3.291 kb on + strand, within Psyr_0003 at 3.291 kb on + strand, within Psyr_0003 at 3.364 kb on + strand, within Psyr_0003 at 3.364 kb on + strand, within Psyr_0003 at 3.364 kb on + strand, within Psyr_0003 at 3.364 kb on + strand, within Psyr_0003 at 3.367 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.395 kb on + strand, within Psyr_0003 at 3.399 kb on + strand, within Psyr_0003 at 3.399 kb on + strand, within Psyr_0003 at 3.399 kb on + strand, within Psyr_0003 at 3.399 kb on + strand, within Psyr_0003 at 3.399 kb on + strand, within Psyr_0003 at 3.480 kb on + strand, within Psyr_0003 at 3.480 kb on + strand, within Psyr_0003 at 3.507 kb on + strand, within Psyr_0003 at 3.507 kb on + strand, within Psyr_0003 at 3.552 kb on + strand, within Psyr_0003 at 3.597 kb on + strand, within Psyr_0003 at 3.597 kb on + strand, within Psyr_0003 at 3.597 kb on + strand, within Psyr_0003 at 3.597 kb on + strand, within Psyr_0003 at 3.601 kb on + strand, within Psyr_0003 at 3.601 kb on + strand, within Psyr_0003 at 3.601 kb on + strand, within Psyr_0003 at 3.601 kb on + strand, within Psyr_0003 at 3.601 kb on + strand, within Psyr_0003 at 3.608 kb on + strand, within Psyr_0003 at 3.608 kb on + strand, within Psyr_0003 at 3.608 kb on + strand, within Psyr_0003 at 3.674 kb on + strand, within Psyr_0003 at 3.690 kb on + strand, within Psyr_0003 at 3.701 kb on + strand at 3.701 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,676 + -2.4 2,698 + -0.5 2,698 + +4.2 2,698 + -2.1 2,698 + +3.4 2,698 + -0.4 2,698 + +0.0 2,698 + -0.8 2,698 + -1.4 2,698 + -0.9 2,698 + -2.3 2,698 + -0.1 2,698 + +3.1 2,698 + +3.1 2,698 + -0.2 2,698 + -0.2 2,698 + -1.8 2,699 - -1.8 2,699 - -1.9 2,699 - -2.6 2,699 - -0.8 2,699 - -3.8 2,699 - -1.5 2,699 - -2.0 2,699 - -4.3 2,699 - -0.1 2,699 - -1.8 2,699 - -2.1 2,699 - -4.0 2,699 - -5.6 2,710 - -0.1 2,827 - Psyr_0003 0.11 -1.5 2,887 + Psyr_0003 0.17 -1.6 3,095 + Psyr_0003 0.36 -2.4 3,095 + Psyr_0003 0.36 -0.1 3,207 + Psyr_0003 0.46 +1.5 3,207 + Psyr_0003 0.46 -2.8 3,289 + Psyr_0003 0.53 -1.5 3,289 + Psyr_0003 0.53 -1.6 3,289 + Psyr_0003 0.53 -0.1 3,289 + Psyr_0003 0.53 -2.2 3,289 + Psyr_0003 0.53 +2.5 3,291 + Psyr_0003 0.53 -0.0 3,291 + Psyr_0003 0.53 -4.7 3,291 + Psyr_0003 0.53 -1.4 3,291 + Psyr_0003 0.53 -1.9 3,291 + Psyr_0003 0.53 -3.2 3,291 + Psyr_0003 0.53 -1.2 3,364 + Psyr_0003 0.60 -0.1 3,364 + Psyr_0003 0.60 -1.9 3,364 + Psyr_0003 0.60 -0.1 3,364 + Psyr_0003 0.60 -2.4 3,367 + Psyr_0003 0.60 -1.6 3,395 + Psyr_0003 0.63 -3.6 3,395 + Psyr_0003 0.63 +3.9 3,395 + Psyr_0003 0.63 -1.0 3,395 + Psyr_0003 0.63 +1.9 3,395 + Psyr_0003 0.63 -3.9 3,395 + Psyr_0003 0.63 -2.7 3,395 + Psyr_0003 0.63 -3.1 3,395 + Psyr_0003 0.63 -1.5 3,395 + Psyr_0003 0.63 +1.9 3,395 + Psyr_0003 0.63 -0.7 3,395 + Psyr_0003 0.63 -0.8 3,395 + Psyr_0003 0.63 -0.1 3,395 + Psyr_0003 0.63 +2.5 3,395 + Psyr_0003 0.63 +0.8 3,395 + Psyr_0003 0.63 -5.4 3,395 + Psyr_0003 0.63 -0.8 3,395 + Psyr_0003 0.63 +0.5 3,399 + Psyr_0003 0.63 -2.6 3,399 + Psyr_0003 0.63 -0.3 3,399 + Psyr_0003 0.63 +2.5 3,399 + Psyr_0003 0.63 +1.5 3,399 + Psyr_0003 0.63 +0.3 3,480 + Psyr_0003 0.71 -0.4 3,480 + Psyr_0003 0.71 -1.2 3,507 + Psyr_0003 0.73 +0.2 3,507 + Psyr_0003 0.73 +0.5 3,552 + Psyr_0003 0.77 -4.9 3,597 + Psyr_0003 0.81 -1.7 3,597 + Psyr_0003 0.81 -1.0 3,597 + Psyr_0003 0.81 -3.0 3,597 + Psyr_0003 0.81 -2.1 3,601 + Psyr_0003 0.82 -0.9 3,601 + Psyr_0003 0.82 -0.9 3,601 + Psyr_0003 0.82 +0.0 3,601 + Psyr_0003 0.82 +1.4 3,601 + Psyr_0003 0.82 -1.8 3,608 + Psyr_0003 0.82 +3.5 3,608 + Psyr_0003 0.82 -1.7 3,608 + Psyr_0003 0.82 -1.3 3,674 + Psyr_0003 0.88 -3.3 3,690 + Psyr_0003 0.90 -2.8 3,701 + -4.2 3,701 + +0.4
Or see this region's nucleotide sequence