Experiment: PYE with copper (II) chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00475 and CCNA_00476 overlap by 4 nucleotides CCNA_00476 and CCNA_00477 are separated by 380 nucleotides CCNA_00477 and CCNA_00478 are separated by 121 nucleotides
CCNA_00475: CCNA_00475 - bacterial conjugation protein, ATP binding domain trwB, at 491,512 to 493,329
_00475
CCNA_00476: CCNA_00476 - hypothetical protein, at 493,326 to 493,709
_00476
CCNA_00477: CCNA_00477 - excisionase, at 494,090 to 494,284
_00477
CCNA_00478: CCNA_00478 - hypothetical protein, at 494,406 to 495,143
_00478
Position (kb) 494
495 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 493.120 kb on + strand, within CCNA_00475 at 493.138 kb on - strand, within CCNA_00475 at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.159 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.181 kb on - strand at 493.191 kb on + strand at 493.199 kb on - strand at 493.270 kb on + strand at 493.270 kb on + strand at 493.270 kb on + strand at 493.270 kb on + strand at 493.278 kb on - strand at 493.278 kb on - strand at 493.314 kb on + strand at 493.335 kb on - strand at 493.400 kb on - strand, within CCNA_00476 at 493.400 kb on - strand, within CCNA_00476 at 493.427 kb on - strand, within CCNA_00476 at 493.432 kb on - strand, within CCNA_00476 at 493.437 kb on - strand, within CCNA_00476 at 493.439 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.454 kb on - strand, within CCNA_00476 at 493.454 kb on - strand, within CCNA_00476 at 493.517 kb on - strand, within CCNA_00476 at 493.520 kb on + strand, within CCNA_00476 at 493.530 kb on + strand, within CCNA_00476 at 493.630 kb on - strand, within CCNA_00476 at 493.639 kb on + strand, within CCNA_00476 at 493.639 kb on + strand, within CCNA_00476 at 493.647 kb on - strand, within CCNA_00476 at 493.734 kb on - strand at 493.782 kb on + strand at 493.790 kb on + strand at 493.798 kb on - strand at 493.798 kb on - strand at 493.811 kb on + strand at 493.819 kb on - strand at 493.820 kb on + strand at 493.835 kb on + strand at 493.848 kb on + strand at 493.848 kb on + strand at 493.848 kb on + strand at 493.852 kb on + strand at 493.856 kb on - strand at 493.858 kb on + strand at 493.866 kb on - strand at 493.945 kb on - strand at 493.945 kb on - strand at 493.945 kb on - strand at 493.945 kb on - strand at 493.951 kb on + strand at 493.951 kb on + strand at 493.959 kb on - strand at 493.959 kb on - strand at 493.968 kb on - strand at 493.968 kb on - strand at 493.995 kb on + strand at 493.995 kb on + strand at 493.995 kb on + strand at 493.995 kb on + strand at 493.995 kb on + strand at 494.000 kb on + strand at 494.000 kb on + strand at 494.003 kb on - strand at 494.003 kb on - strand at 494.003 kb on - strand at 494.003 kb on - strand at 494.003 kb on - strand at 494.003 kb on - strand at 494.062 kb on - strand at 494.093 kb on + strand at 494.131 kb on - strand, within CCNA_00477 at 494.135 kb on + strand, within CCNA_00477 at 494.154 kb on + strand, within CCNA_00477 at 494.181 kb on + strand, within CCNA_00477 at 494.181 kb on + strand, within CCNA_00477 at 494.181 kb on + strand, within CCNA_00477 at 494.181 kb on + strand, within CCNA_00477 at 494.181 kb on + strand, within CCNA_00477 at 494.181 kb on + strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.199 kb on - strand, within CCNA_00477 at 494.199 kb on - strand, within CCNA_00477 at 494.223 kb on + strand, within CCNA_00477 at 494.226 kb on + strand, within CCNA_00477 at 494.231 kb on - strand, within CCNA_00477 at 494.236 kb on + strand, within CCNA_00477 at 494.243 kb on + strand, within CCNA_00477 at 494.243 kb on + strand, within CCNA_00477 at 494.243 kb on + strand, within CCNA_00477 at 494.243 kb on + strand, within CCNA_00477 at 494.251 kb on - strand, within CCNA_00477 at 494.251 kb on - strand, within CCNA_00477 at 494.253 kb on + strand, within CCNA_00477 at 494.263 kb on + strand, within CCNA_00477 at 494.284 kb on + strand at 494.287 kb on + strand at 494.292 kb on + strand at 494.292 kb on + strand at 494.292 kb on + strand at 494.295 kb on - strand at 494.295 kb on - strand at 494.295 kb on - strand at 494.300 kb on - strand at 494.300 kb on - strand at 494.313 kb on + strand at 494.313 kb on + strand at 494.313 kb on + strand at 494.313 kb on + strand at 494.321 kb on - strand at 494.321 kb on - strand at 494.326 kb on - strand at 494.343 kb on - strand at 494.371 kb on + strand at 494.379 kb on - strand at 494.411 kb on - strand at 494.411 kb on - strand at 494.411 kb on - strand at 494.411 kb on - strand at 494.428 kb on + strand at 494.428 kb on + strand at 494.436 kb on - strand at 494.436 kb on - strand at 494.454 kb on + strand at 494.469 kb on - strand at 494.512 kb on - strand, within CCNA_00478 at 494.567 kb on - strand, within CCNA_00478 at 494.572 kb on + strand, within CCNA_00478 at 494.573 kb on + strand, within CCNA_00478 at 494.594 kb on + strand, within CCNA_00478 at 494.602 kb on - strand, within CCNA_00478 at 494.602 kb on - strand, within CCNA_00478 at 494.603 kb on - strand, within CCNA_00478 at 494.603 kb on - strand, within CCNA_00478 at 494.613 kb on - strand, within CCNA_00478 at 494.631 kb on + strand, within CCNA_00478 at 494.790 kb on - strand, within CCNA_00478 at 494.823 kb on - strand, within CCNA_00478 at 494.862 kb on + strand, within CCNA_00478 at 494.862 kb on + strand, within CCNA_00478 at 494.862 kb on + strand, within CCNA_00478 at 494.870 kb on - strand, within CCNA_00478 at 494.870 kb on - strand, within CCNA_00478 at 494.939 kb on + strand, within CCNA_00478 at 494.982 kb on + strand, within CCNA_00478 at 495.012 kb on + strand, within CCNA_00478 at 495.061 kb on + strand, within CCNA_00478 at 495.215 kb on + strand at 495.245 kb on + strand at 495.245 kb on + strand at 495.253 kb on - strand at 495.253 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with copper (II) chloride 0.25 mM remove 493,120 + CCNA_00475 0.88 -0.1 493,138 - CCNA_00475 0.89 +1.2 493,156 + -1.1 493,156 + -1.3 493,156 + +1.0 493,156 + +0.8 493,156 + -1.1 493,156 + -0.6 493,156 + -0.2 493,156 + -2.1 493,156 + +0.8 493,156 + -2.7 493,156 + -0.5 493,156 + -1.0 493,156 + +1.5 493,156 + -1.4 493,156 + -0.2 493,156 + +0.4 493,156 + -0.2 493,156 + -1.2 493,156 + +1.1 493,156 + +0.6 493,159 - +0.8 493,164 - -1.5 493,164 - -0.2 493,164 - +1.4 493,164 - -2.4 493,164 - -1.5 493,164 - -1.0 493,164 - -0.1 493,164 - -1.3 493,164 - -1.3 493,164 - -0.3 493,164 - +0.5 493,164 - -0.2 493,164 - +0.2 493,164 - -0.2 493,164 - -1.2 493,181 - +0.8 493,191 + -0.2 493,199 - -0.5 493,270 + -1.0 493,270 + -1.5 493,270 + -1.1 493,270 + -0.5 493,278 - +0.3 493,278 - +1.0 493,314 + -0.1 493,335 - -0.3 493,400 - CCNA_00476 0.19 -2.0 493,400 - CCNA_00476 0.19 -1.0 493,427 - CCNA_00476 0.26 -1.2 493,432 - CCNA_00476 0.28 -0.0 493,437 - CCNA_00476 0.29 -0.9 493,439 + CCNA_00476 0.29 -0.3 493,446 + CCNA_00476 0.31 -0.9 493,446 + CCNA_00476 0.31 +1.5 493,446 + CCNA_00476 0.31 -0.8 493,446 + CCNA_00476 0.31 -1.3 493,446 + CCNA_00476 0.31 +1.4 493,446 + CCNA_00476 0.31 +1.1 493,454 - CCNA_00476 0.33 +1.4 493,454 - CCNA_00476 0.33 +0.8 493,517 - CCNA_00476 0.50 -1.6 493,520 + CCNA_00476 0.51 -1.2 493,530 + CCNA_00476 0.53 +0.0 493,630 - CCNA_00476 0.79 -1.7 493,639 + CCNA_00476 0.82 -1.9 493,639 + CCNA_00476 0.82 -0.1 493,647 - CCNA_00476 0.84 -0.9 493,734 - -1.1 493,782 + +0.5 493,790 + -0.6 493,798 - +0.4 493,798 - +0.8 493,811 + -1.1 493,819 - -2.9 493,820 + +0.5 493,835 + -0.5 493,848 + +1.8 493,848 + -0.7 493,848 + -1.1 493,852 + -0.6 493,856 - -0.9 493,858 + -0.3 493,866 - +0.8 493,945 - +1.3 493,945 - +0.8 493,945 - +1.8 493,945 - +1.4 493,951 + -2.7 493,951 + +2.8 493,959 - -1.8 493,959 - -0.6 493,968 - +0.5 493,968 - -1.0 493,995 + +0.1 493,995 + -1.3 493,995 + +0.0 493,995 + -0.3 493,995 + +0.3 494,000 + -0.6 494,000 + -1.0 494,003 - -1.8 494,003 - +0.0 494,003 - +0.9 494,003 - -1.3 494,003 - -0.7 494,003 - -0.3 494,062 - -1.4 494,093 + -1.6 494,131 - CCNA_00477 0.21 -2.7 494,135 + CCNA_00477 0.23 +0.1 494,154 + CCNA_00477 0.33 -0.9 494,181 + CCNA_00477 0.47 -0.8 494,181 + CCNA_00477 0.47 -2.0 494,181 + CCNA_00477 0.47 -1.5 494,181 + CCNA_00477 0.47 -1.4 494,181 + CCNA_00477 0.47 -1.7 494,181 + CCNA_00477 0.47 -1.7 494,189 - CCNA_00477 0.51 -1.1 494,189 - CCNA_00477 0.51 -0.6 494,189 - CCNA_00477 0.51 -0.2 494,189 - CCNA_00477 0.51 +0.7 494,189 - CCNA_00477 0.51 -0.7 494,189 - CCNA_00477 0.51 -1.0 494,189 - CCNA_00477 0.51 -0.9 494,189 - CCNA_00477 0.51 -1.6 494,189 - CCNA_00477 0.51 -0.3 494,189 - CCNA_00477 0.51 -0.5 494,189 - CCNA_00477 0.51 -0.0 494,189 - CCNA_00477 0.51 +0.4 494,189 - CCNA_00477 0.51 +0.3 494,189 - CCNA_00477 0.51 +0.0 494,199 - CCNA_00477 0.56 -0.9 494,199 - CCNA_00477 0.56 +0.2 494,223 + CCNA_00477 0.68 -1.8 494,226 + CCNA_00477 0.70 +0.7 494,231 - CCNA_00477 0.72 -1.9 494,236 + CCNA_00477 0.75 -1.2 494,243 + CCNA_00477 0.78 -1.9 494,243 + CCNA_00477 0.78 -1.8 494,243 + CCNA_00477 0.78 +0.2 494,243 + CCNA_00477 0.78 +0.0 494,251 - CCNA_00477 0.83 -0.6 494,251 - CCNA_00477 0.83 -0.5 494,253 + CCNA_00477 0.84 -1.6 494,263 + CCNA_00477 0.89 -0.1 494,284 + -0.5 494,287 + -1.5 494,292 + -2.6 494,292 + +1.4 494,292 + +1.8 494,295 - -0.6 494,295 - +0.0 494,295 - +0.1 494,300 - -0.5 494,300 - -0.3 494,313 + -0.1 494,313 + -1.7 494,313 + -1.5 494,313 + +1.0 494,321 - +0.1 494,321 - -0.5 494,326 - +1.0 494,343 - -0.1 494,371 + -0.9 494,379 - -0.3 494,411 - -1.5 494,411 - -1.6 494,411 - -1.2 494,411 - -0.1 494,428 + +1.8 494,428 + -1.5 494,436 - -1.1 494,436 - +0.5 494,454 + +1.4 494,469 - -0.4 494,512 - CCNA_00478 0.14 -1.2 494,567 - CCNA_00478 0.22 +0.4 494,572 + CCNA_00478 0.22 +0.0 494,573 + CCNA_00478 0.23 -0.5 494,594 + CCNA_00478 0.25 -0.2 494,602 - CCNA_00478 0.27 +0.8 494,602 - CCNA_00478 0.27 -1.8 494,603 - CCNA_00478 0.27 -0.8 494,603 - CCNA_00478 0.27 -0.2 494,613 - CCNA_00478 0.28 -1.1 494,631 + CCNA_00478 0.30 -1.6 494,790 - CCNA_00478 0.52 -2.5 494,823 - CCNA_00478 0.57 -1.4 494,862 + CCNA_00478 0.62 +0.0 494,862 + CCNA_00478 0.62 +0.4 494,862 + CCNA_00478 0.62 -0.1 494,870 - CCNA_00478 0.63 +0.8 494,870 - CCNA_00478 0.63 -0.6 494,939 + CCNA_00478 0.72 -2.1 494,982 + CCNA_00478 0.78 -2.1 495,012 + CCNA_00478 0.82 -0.2 495,061 + CCNA_00478 0.89 -2.2 495,215 + -0.1 495,245 + -1.7 495,245 + -2.7 495,253 - -0.4 495,253 - +0.4
Or see this region's nucleotide sequence