Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3744 and Rru_A3745 are separated by 80 nucleotides Rru_A3745 and Rru_A3746 are separated by 146 nucleotides Rru_A3746 and Rru_A3747 are separated by 27 nucleotides Rru_A3747 and Rru_A3748 are separated by 3 nucleotides
Rru_A3744: Rru_A3744 - Predicted signal-transduction protein containing CBS domains (NCBI), at 4,293,572 to 4,294,018
_A3744
Rru_A3745: Rru_A3745 - hypothetical protein (NCBI), at 4,294,099 to 4,294,671
_A3745
Rru_A3746: Rru_A3746 - Ribosomal protein L25 (NCBI), at 4,294,818 to 4,295,450
_A3746
Rru_A3747: Rru_A3747 - Aminoacyl-tRNA hydrolase (NCBI), at 4,295,478 to 4,296,209
_A3747
Rru_A3748: Rru_A3748 - hypothetical protein (NCBI), at 4,296,213 to 4,297,316
_A3748
Position (kb) 4294
4295
4296 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4293.872 kb on + strand, within Rru_A3744 at 4293.935 kb on + strand, within Rru_A3744 at 4293.993 kb on - strand at 4294.004 kb on + strand at 4294.004 kb on + strand at 4294.005 kb on - strand at 4294.219 kb on + strand, within Rru_A3745 at 4294.219 kb on + strand, within Rru_A3745 at 4294.219 kb on + strand, within Rru_A3745 at 4294.219 kb on + strand, within Rru_A3745 at 4296.401 kb on + strand, within Rru_A3748 at 4296.402 kb on - strand, within Rru_A3748 at 4296.402 kb on - strand, within Rru_A3748 at 4296.402 kb on - strand, within Rru_A3748
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 4,293,872 + Rru_A3744 0.67 -2.8 4,293,935 + Rru_A3744 0.81 -1.9 4,293,993 - -1.6 4,294,004 + -1.1 4,294,004 + +0.9 4,294,005 - -0.8 4,294,219 + Rru_A3745 0.21 -2.0 4,294,219 + Rru_A3745 0.21 +1.1 4,294,219 + Rru_A3745 0.21 -1.3 4,294,219 + Rru_A3745 0.21 -0.5 4,296,401 + Rru_A3748 0.17 -2.3 4,296,402 - Rru_A3748 0.17 +0.1 4,296,402 - Rru_A3748 0.17 +0.8 4,296,402 - Rru_A3748 0.17 -2.4
Or see this region's nucleotide sequence