Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3589 and Rru_A3590 are separated by 56 nucleotides Rru_A3590 and Rru_A3591 are separated by 10 nucleotides Rru_A3591 and Rru_A3592 are separated by 107 nucleotides Rru_A3592 and Rru_A3593 are separated by 24 nucleotides Rru_A3593 and Rru_A3594 are separated by 73 nucleotides
Rru_A3589: Rru_A3589 - Mg chelatase-related protein (NCBI), at 4,132,544 to 4,134,064
_A3589
Rru_A3590: Rru_A3590 - hypothetical protein (NCBI), at 4,134,121 to 4,134,765
_A3590
Rru_A3591: Rru_A3591 - hypothetical protein (NCBI), at 4,134,776 to 4,135,495
_A3591
Rru_A3592: Rru_A3592 - Histidine triad (HIT) protein (NCBI), at 4,135,603 to 4,135,965
_A3592
Rru_A3593: Rru_A3593 - Phosphoribosyl-ATP pyrophosphohydrolase (NCBI), at 4,135,990 to 4,136,343
_A3593
Rru_A3594: Rru_A3594 - Histidine biosynthesis protein HisF (NCBI), at 4,136,417 to 4,137,175
_A3594
Position (kb)
4134
4135
4136 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4133.965 kb on + strand at 4133.965 kb on + strand at 4133.965 kb on + strand at 4134.062 kb on + strand at 4134.062 kb on + strand at 4134.063 kb on - strand at 4134.360 kb on + strand, within Rru_A3590 at 4134.427 kb on + strand, within Rru_A3590 at 4134.428 kb on - strand, within Rru_A3590 at 4134.448 kb on + strand, within Rru_A3590 at 4134.607 kb on + strand, within Rru_A3590 at 4134.607 kb on + strand, within Rru_A3590 at 4134.608 kb on - strand, within Rru_A3590 at 4134.608 kb on - strand, within Rru_A3590 at 4134.608 kb on - strand, within Rru_A3590 at 4134.608 kb on - strand, within Rru_A3590 at 4134.608 kb on - strand, within Rru_A3590 at 4134.763 kb on + strand at 4134.777 kb on + strand at 4134.784 kb on - strand at 4134.784 kb on - strand at 4134.784 kb on - strand at 4134.798 kb on + strand at 4134.799 kb on - strand at 4134.799 kb on - strand at 4135.010 kb on - strand, within Rru_A3591 at 4135.044 kb on + strand, within Rru_A3591 at 4135.044 kb on + strand, within Rru_A3591 at 4135.044 kb on + strand, within Rru_A3591 at 4135.044 kb on + strand, within Rru_A3591 at 4135.044 kb on + strand, within Rru_A3591 at 4135.045 kb on - strand, within Rru_A3591 at 4135.205 kb on - strand, within Rru_A3591 at 4135.434 kb on + strand at 4135.434 kb on + strand at 4135.944 kb on + strand at 4135.944 kb on + strand at 4135.944 kb on + strand at 4135.944 kb on + strand at 4135.944 kb on + strand at 4135.945 kb on - strand at 4135.945 kb on - strand at 4135.945 kb on - strand at 4135.945 kb on - strand at 4135.958 kb on + strand at 4135.959 kb on - strand at 4136.105 kb on + strand, within Rru_A3593 at 4136.238 kb on - strand, within Rru_A3593 at 4136.419 kb on - strand at 4136.449 kb on + strand at 4136.473 kb on + strand at 4136.474 kb on - strand at 4136.475 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 4,133,965 + +1.0 4,133,965 + +1.0 4,133,965 + -0.3 4,134,062 + -0.2 4,134,062 + -0.1 4,134,063 - -1.1 4,134,360 + Rru_A3590 0.37 -2.9 4,134,427 + Rru_A3590 0.47 -0.7 4,134,428 - Rru_A3590 0.48 +1.7 4,134,448 + Rru_A3590 0.51 +2.1 4,134,607 + Rru_A3590 0.75 +0.6 4,134,607 + Rru_A3590 0.75 +2.5 4,134,608 - Rru_A3590 0.76 +1.3 4,134,608 - Rru_A3590 0.76 -2.8 4,134,608 - Rru_A3590 0.76 +1.2 4,134,608 - Rru_A3590 0.76 -1.7 4,134,608 - Rru_A3590 0.76 -1.2 4,134,763 + +0.6 4,134,777 + -0.5 4,134,784 - -1.6 4,134,784 - -2.5 4,134,784 - +0.7 4,134,798 + +0.7 4,134,799 - +0.9 4,134,799 - +0.1 4,135,010 - Rru_A3591 0.33 +1.5 4,135,044 + Rru_A3591 0.37 -0.2 4,135,044 + Rru_A3591 0.37 +2.8 4,135,044 + Rru_A3591 0.37 +0.2 4,135,044 + Rru_A3591 0.37 +0.6 4,135,044 + Rru_A3591 0.37 +0.3 4,135,045 - Rru_A3591 0.37 +0.8 4,135,205 - Rru_A3591 0.60 -1.4 4,135,434 + +1.4 4,135,434 + +0.5 4,135,944 + -1.7 4,135,944 + +0.7 4,135,944 + +2.0 4,135,944 + +1.0 4,135,944 + -2.8 4,135,945 - -1.9 4,135,945 - -1.8 4,135,945 - +0.9 4,135,945 - -3.3 4,135,958 + +0.4 4,135,959 - -0.1 4,136,105 + Rru_A3593 0.32 -1.9 4,136,238 - Rru_A3593 0.70 -1.6 4,136,419 - +1.3 4,136,449 + -3.9 4,136,473 + -1.1 4,136,474 - -3.2 4,136,475 + -1.2
Or see this region's nucleotide sequence