Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3497 and Rru_A3498 are separated by 34 nucleotides Rru_A3498 and Rru_A3499 are separated by 18 nucleotides Rru_A3499 and Rru_A3500 are separated by 68 nucleotides Rru_A3500 and Rru_A3501 are separated by 39 nucleotides
Rru_A3497: Rru_A3497 - Disulphide bond formation protein DsbB (NCBI), at 4,026,475 to 4,027,008
_A3497
Rru_A3498: Rru_A3498 - Ubiquinone biosynthesis protein COQ7 (NCBI), at 4,027,043 to 4,027,681
_A3498
Rru_A3499: Rru_A3499 - hypothetical protein (NCBI), at 4,027,700 to 4,028,227
_A3499
Rru_A3500: Rru_A3500 - Cobalamin (vitamin B12) biosynthesis CbiM protein (NCBI), at 4,028,296 to 4,028,931
_A3500
Rru_A3501: Rru_A3501 - lipoprotein, putative (NCBI), at 4,028,971 to 4,029,768
_A3501
Position (kb)
4027
4028
4029 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4027.005 kb on - strand at 4027.006 kb on + strand at 4027.006 kb on + strand at 4027.007 kb on - strand at 4027.007 kb on - strand at 4027.007 kb on - strand at 4027.007 kb on - strand at 4027.007 kb on - strand at 4027.007 kb on - strand at 4027.701 kb on + strand at 4027.756 kb on + strand, within Rru_A3499 at 4027.756 kb on + strand, within Rru_A3499 at 4027.757 kb on - strand, within Rru_A3499 at 4027.757 kb on - strand, within Rru_A3499 at 4027.759 kb on - strand, within Rru_A3499 at 4028.050 kb on + strand, within Rru_A3499 at 4028.184 kb on + strand at 4028.236 kb on + strand at 4028.237 kb on - strand at 4028.237 kb on - strand at 4028.925 kb on + strand at 4028.972 kb on + strand at 4028.972 kb on + strand at 4028.973 kb on - strand at 4028.986 kb on - strand at 4028.995 kb on - strand at 4029.102 kb on + strand, within Rru_A3501 at 4029.103 kb on - strand, within Rru_A3501 at 4029.202 kb on + strand, within Rru_A3501 at 4029.203 kb on - strand, within Rru_A3501 at 4029.203 kb on - strand, within Rru_A3501
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 4,027,005 - +1.5 4,027,006 + +0.7 4,027,006 + -1.0 4,027,007 - +0.6 4,027,007 - +0.5 4,027,007 - +0.9 4,027,007 - -0.7 4,027,007 - -0.2 4,027,007 - -1.9 4,027,701 + +0.3 4,027,756 + Rru_A3499 0.11 -0.0 4,027,756 + Rru_A3499 0.11 -0.4 4,027,757 - Rru_A3499 0.11 +2.2 4,027,757 - Rru_A3499 0.11 -2.9 4,027,759 - Rru_A3499 0.11 +0.7 4,028,050 + Rru_A3499 0.66 -0.3 4,028,184 + +0.1 4,028,236 + +0.6 4,028,237 - +1.7 4,028,237 - -1.1 4,028,925 + -1.5 4,028,972 + +0.5 4,028,972 + -1.8 4,028,973 - +0.0 4,028,986 - -0.4 4,028,995 - -0.2 4,029,102 + Rru_A3501 0.16 +0.3 4,029,103 - Rru_A3501 0.17 +0.6 4,029,202 + Rru_A3501 0.29 -0.2 4,029,203 - Rru_A3501 0.29 -0.1 4,029,203 - Rru_A3501 0.29 -2.7
Or see this region's nucleotide sequence