Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3046 and Rru_A3047 are separated by 132 nucleotides Rru_A3047 and Rru_A3048 are separated by 47 nucleotides
Rru_A3046: Rru_A3046 - Radical SAM (NCBI), at 3,509,231 to 3,510,112
_A3046
Rru_A3047: Rru_A3047 - hypothetical protein (NCBI), at 3,510,245 to 3,510,682
_A3047
Rru_A3048: Rru_A3048 - Glycine dehydrogenase (decarboxylating) (NCBI), at 3,510,730 to 3,512,244
_A3048
Position (kb)
3510
3511 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3509.521 kb on - strand, within Rru_A3046 at 3509.521 kb on - strand, within Rru_A3046 at 3509.725 kb on + strand, within Rru_A3046 at 3509.726 kb on - strand, within Rru_A3046 at 3509.726 kb on - strand, within Rru_A3046 at 3509.727 kb on + strand, within Rru_A3046 at 3509.727 kb on + strand, within Rru_A3046 at 3509.727 kb on + strand, within Rru_A3046 at 3509.727 kb on + strand, within Rru_A3046 at 3509.728 kb on - strand, within Rru_A3046 at 3509.728 kb on - strand, within Rru_A3046 at 3509.728 kb on - strand, within Rru_A3046 at 3509.728 kb on - strand, within Rru_A3046 at 3509.789 kb on - strand, within Rru_A3046 at 3509.805 kb on + strand, within Rru_A3046 at 3509.806 kb on - strand, within Rru_A3046 at 3509.806 kb on - strand, within Rru_A3046 at 3509.806 kb on - strand, within Rru_A3046 at 3509.985 kb on + strand, within Rru_A3046 at 3509.986 kb on - strand, within Rru_A3046 at 3510.000 kb on + strand, within Rru_A3046 at 3510.000 kb on + strand, within Rru_A3046 at 3510.053 kb on - strand at 3510.094 kb on + strand at 3510.094 kb on + strand at 3510.094 kb on + strand at 3510.095 kb on - strand at 3510.095 kb on - strand at 3510.095 kb on - strand at 3510.099 kb on + strand at 3510.099 kb on + strand at 3510.100 kb on - strand at 3510.100 kb on - strand at 3510.174 kb on + strand at 3510.177 kb on - strand at 3510.177 kb on - strand at 3510.177 kb on - strand at 3510.177 kb on - strand at 3510.255 kb on + strand at 3510.256 kb on - strand at 3510.326 kb on + strand, within Rru_A3047 at 3510.327 kb on - strand, within Rru_A3047 at 3510.327 kb on - strand, within Rru_A3047 at 3510.327 kb on - strand, within Rru_A3047 at 3510.327 kb on - strand, within Rru_A3047 at 3510.327 kb on - strand, within Rru_A3047 at 3510.329 kb on + strand, within Rru_A3047 at 3510.330 kb on - strand, within Rru_A3047 at 3510.425 kb on + strand, within Rru_A3047 at 3510.426 kb on - strand, within Rru_A3047 at 3510.681 kb on - strand at 3510.732 kb on - strand at 3510.971 kb on + strand, within Rru_A3048 at 3510.971 kb on + strand, within Rru_A3048 at 3510.972 kb on - strand, within Rru_A3048 at 3510.972 kb on - strand, within Rru_A3048 at 3510.989 kb on + strand, within Rru_A3048 at 3510.989 kb on + strand, within Rru_A3048 at 3510.989 kb on + strand, within Rru_A3048 at 3511.130 kb on + strand, within Rru_A3048 at 3511.131 kb on - strand, within Rru_A3048 at 3511.197 kb on - strand, within Rru_A3048 at 3511.197 kb on - strand, within Rru_A3048 at 3511.328 kb on + strand, within Rru_A3048 at 3511.328 kb on + strand, within Rru_A3048 at 3511.329 kb on - strand, within Rru_A3048 at 3511.431 kb on + strand, within Rru_A3048 at 3511.431 kb on + strand, within Rru_A3048 at 3511.431 kb on + strand, within Rru_A3048 at 3511.432 kb on - strand, within Rru_A3048 at 3511.432 kb on - strand, within Rru_A3048 at 3511.432 kb on - strand, within Rru_A3048 at 3511.432 kb on - strand, within Rru_A3048 at 3511.503 kb on + strand, within Rru_A3048 at 3511.505 kb on + strand, within Rru_A3048 at 3511.505 kb on + strand, within Rru_A3048 at 3511.506 kb on - strand, within Rru_A3048
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 3,509,521 - Rru_A3046 0.33 +0.4 3,509,521 - Rru_A3046 0.33 -3.4 3,509,725 + Rru_A3046 0.56 +0.7 3,509,726 - Rru_A3046 0.56 -2.0 3,509,726 - Rru_A3046 0.56 -0.7 3,509,727 + Rru_A3046 0.56 +1.0 3,509,727 + Rru_A3046 0.56 +0.5 3,509,727 + Rru_A3046 0.56 +1.5 3,509,727 + Rru_A3046 0.56 +0.2 3,509,728 - Rru_A3046 0.56 -1.2 3,509,728 - Rru_A3046 0.56 -4.3 3,509,728 - Rru_A3046 0.56 +1.1 3,509,728 - Rru_A3046 0.56 -0.7 3,509,789 - Rru_A3046 0.63 -1.9 3,509,805 + Rru_A3046 0.65 +0.2 3,509,806 - Rru_A3046 0.65 -1.6 3,509,806 - Rru_A3046 0.65 +0.1 3,509,806 - Rru_A3046 0.65 +0.2 3,509,985 + Rru_A3046 0.85 -1.0 3,509,986 - Rru_A3046 0.86 -0.6 3,510,000 + Rru_A3046 0.87 -5.4 3,510,000 + Rru_A3046 0.87 -1.3 3,510,053 - +0.6 3,510,094 + -2.1 3,510,094 + +0.1 3,510,094 + -3.4 3,510,095 - -0.7 3,510,095 - -1.8 3,510,095 - -1.0 3,510,099 + -0.4 3,510,099 + +1.6 3,510,100 - -1.5 3,510,100 - +1.1 3,510,174 + -1.7 3,510,177 - -0.5 3,510,177 - -1.8 3,510,177 - +0.0 3,510,177 - -1.4 3,510,255 + +0.2 3,510,256 - +0.7 3,510,326 + Rru_A3047 0.18 -0.7 3,510,327 - Rru_A3047 0.19 -1.1 3,510,327 - Rru_A3047 0.19 +1.3 3,510,327 - Rru_A3047 0.19 +0.8 3,510,327 - Rru_A3047 0.19 +1.1 3,510,327 - Rru_A3047 0.19 +0.5 3,510,329 + Rru_A3047 0.19 -2.4 3,510,330 - Rru_A3047 0.19 +0.1 3,510,425 + Rru_A3047 0.41 +1.3 3,510,426 - Rru_A3047 0.41 +1.0 3,510,681 - -1.1 3,510,732 - -2.0 3,510,971 + Rru_A3048 0.16 +0.1 3,510,971 + Rru_A3048 0.16 -1.2 3,510,972 - Rru_A3048 0.16 -2.2 3,510,972 - Rru_A3048 0.16 -0.3 3,510,989 + Rru_A3048 0.17 -1.7 3,510,989 + Rru_A3048 0.17 +0.5 3,510,989 + Rru_A3048 0.17 -0.9 3,511,130 + Rru_A3048 0.26 -2.5 3,511,131 - Rru_A3048 0.26 +0.1 3,511,197 - Rru_A3048 0.31 -0.1 3,511,197 - Rru_A3048 0.31 +1.8 3,511,328 + Rru_A3048 0.39 -0.2 3,511,328 + Rru_A3048 0.39 +0.2 3,511,329 - Rru_A3048 0.40 +2.3 3,511,431 + Rru_A3048 0.46 +0.5 3,511,431 + Rru_A3048 0.46 -1.2 3,511,431 + Rru_A3048 0.46 +0.6 3,511,432 - Rru_A3048 0.46 -0.5 3,511,432 - Rru_A3048 0.46 +0.1 3,511,432 - Rru_A3048 0.46 -0.7 3,511,432 - Rru_A3048 0.46 -0.7 3,511,503 + Rru_A3048 0.51 +2.1 3,511,505 + Rru_A3048 0.51 -0.9 3,511,505 + Rru_A3048 0.51 +0.1 3,511,506 - Rru_A3048 0.51 -0.4
Or see this region's nucleotide sequence