Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2351 and Rru_AR0041 are separated by 449 nucleotides Rru_AR0041 and Rru_AR0042 are separated by 95 nucleotides Rru_AR0042 and Rru_AR0043 are separated by 119 nucleotides
Rru_A2351: Rru_A2351 - Macrocin-O-methyltransferase (NCBI), at 2,729,598 to 2,731,271
_A2351
Rru_AR0041: Rru_AR0041 - tRNA-Met (NCBI), at 2,731,721 to 2,731,797
_AR0041
Rru_AR0042: Rru_AR0042 - 5S ribosomal RNA (NCBI), at 2,731,893 to 2,732,007
_AR0042
Rru_AR0043: Rru_AR0043 - 23S ribosomal RNA (NCBI), at 2,732,127 to 2,734,884
_AR0043
Position (kb)
2731
2732
2733 Strain fitness (log2 ratio)
-2
-1
0
1 at 2730.945 kb on - strand, within Rru_A2351 at 2731.047 kb on + strand, within Rru_A2351 at 2731.047 kb on + strand, within Rru_A2351 at 2731.047 kb on + strand, within Rru_A2351 at 2731.513 kb on + strand at 2731.514 kb on - strand at 2731.544 kb on - strand at 2731.551 kb on + strand at 2731.551 kb on + strand at 2731.552 kb on - strand at 2731.799 kb on + strand at 2731.908 kb on - strand, within Rru_AR0042 at 2731.908 kb on - strand, within Rru_AR0042 at 2731.908 kb on - strand, within Rru_AR0042 at 2731.908 kb on - strand, within Rru_AR0042
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 2,730,945 - Rru_A2351 0.80 +0.4 2,731,047 + Rru_A2351 0.87 +1.2 2,731,047 + Rru_A2351 0.87 -0.5 2,731,047 + Rru_A2351 0.87 +0.1 2,731,513 + +0.9 2,731,514 - +0.3 2,731,544 - +0.6 2,731,551 + +1.0 2,731,551 + +0.7 2,731,552 - +0.2 2,731,799 + -0.4 2,731,908 - Rru_AR0042 0.13 -2.3 2,731,908 - Rru_AR0042 0.13 -0.8 2,731,908 - Rru_AR0042 0.13 +0.2 2,731,908 - Rru_AR0042 0.13 +0.3
Or see this region's nucleotide sequence