Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2133 and Rru_A2134 are separated by 173 nucleotides Rru_A2134 and Rru_A2135 are separated by 189 nucleotides
Rru_A2133: Rru_A2133 - glycosyl transferase, group 2 family protein (NCBI), at 2,477,446 to 2,479,488
_A2133
Rru_A2134: Rru_A2134 - hypothetical protein (NCBI), at 2,479,662 to 2,480,141
_A2134
Rru_A2135: Rru_A2135 - Twin-arginine translocation pathway signal (NCBI), at 2,480,331 to 2,481,917
_A2135
Position (kb)
2479
2480
2481 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2479.531 kb on - strand at 2479.533 kb on + strand at 2479.534 kb on - strand at 2479.534 kb on - strand at 2479.534 kb on - strand at 2479.583 kb on + strand at 2479.583 kb on + strand at 2479.583 kb on + strand at 2479.583 kb on + strand at 2479.583 kb on + strand at 2479.584 kb on - strand at 2479.584 kb on - strand at 2479.584 kb on - strand at 2479.584 kb on - strand at 2479.584 kb on - strand at 2479.584 kb on - strand at 2479.584 kb on - strand at 2479.593 kb on + strand at 2480.195 kb on - strand at 2480.198 kb on - strand at 2480.199 kb on + strand at 2480.200 kb on - strand at 2480.200 kb on - strand at 2480.200 kb on - strand at 2480.332 kb on + strand at 2480.332 kb on + strand at 2480.333 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 2,479,531 - -3.3 2,479,533 + -0.1 2,479,534 - -0.5 2,479,534 - +2.4 2,479,534 - +0.9 2,479,583 + -2.5 2,479,583 + -2.8 2,479,583 + -1.9 2,479,583 + -0.3 2,479,583 + -2.1 2,479,584 - -0.6 2,479,584 - -0.8 2,479,584 - +0.8 2,479,584 - -0.5 2,479,584 - -1.7 2,479,584 - +0.6 2,479,584 - -1.9 2,479,593 + +0.8 2,480,195 - +1.8 2,480,198 - +0.1 2,480,199 + -1.6 2,480,200 - -0.0 2,480,200 - -0.4 2,480,200 - +0.0 2,480,332 + -0.9 2,480,332 + -1.9 2,480,333 - -1.5
Or see this region's nucleotide sequence