Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1388 and Rru_A1389 are separated by 41 nucleotides Rru_A1389 and Rru_A1390 overlap by 4 nucleotides Rru_A1390 and Rru_A1391 overlap by 4 nucleotides Rru_A1391 and Rru_A1392 are separated by 88 nucleotides
Rru_A1388: Rru_A1388 - Transcriptional Regulator, NifA subfamily, Fis Family (NCBI), at 1,639,371 to 1,640,972
_A1388
Rru_A1389: Rru_A1389 - NAD(+)--dinitrogen-reductase ADP-D-ribosyltransferase (NCBI), at 1,641,014 to 1,641,925
_A1389
Rru_A1390: Rru_A1390 - hypothetical protein (NCBI), at 1,641,922 to 1,642,479
_A1390
Rru_A1391: Rru_A1391 - hypothetical protein (NCBI), at 1,642,476 to 1,643,198
_A1391
Rru_A1392: Rru_A1392 - Nitrogenase (NCBI), at 1,643,287 to 1,644,675
_A1392
Position (kb)
1641
1642
1643 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1640.937 kb on + strand at 1640.938 kb on - strand at 1640.949 kb on + strand at 1640.949 kb on + strand at 1640.950 kb on - strand at 1640.950 kb on - strand at 1640.958 kb on + strand at 1641.058 kb on + strand at 1641.059 kb on - strand at 1641.059 kb on - strand at 1641.290 kb on + strand, within Rru_A1389 at 1641.290 kb on + strand, within Rru_A1389 at 1641.290 kb on + strand, within Rru_A1389 at 1641.291 kb on - strand, within Rru_A1389 at 1641.291 kb on - strand, within Rru_A1389 at 1641.533 kb on + strand, within Rru_A1389 at 1641.534 kb on - strand, within Rru_A1389 at 1641.599 kb on + strand, within Rru_A1389 at 1641.599 kb on + strand, within Rru_A1389 at 1641.600 kb on - strand, within Rru_A1389 at 1641.600 kb on - strand, within Rru_A1389 at 1641.606 kb on - strand, within Rru_A1389 at 1641.662 kb on + strand, within Rru_A1389 at 1641.740 kb on - strand, within Rru_A1389 at 1641.740 kb on - strand, within Rru_A1389 at 1641.900 kb on - strand at 1641.923 kb on + strand at 1641.924 kb on - strand at 1641.924 kb on - strand at 1642.034 kb on + strand, within Rru_A1390 at 1642.035 kb on - strand, within Rru_A1390 at 1642.035 kb on - strand, within Rru_A1390 at 1642.035 kb on - strand, within Rru_A1390 at 1642.035 kb on - strand, within Rru_A1390 at 1642.086 kb on - strand, within Rru_A1390 at 1642.131 kb on - strand, within Rru_A1390 at 1642.191 kb on - strand, within Rru_A1390 at 1642.227 kb on + strand, within Rru_A1390 at 1642.227 kb on + strand, within Rru_A1390 at 1642.227 kb on + strand, within Rru_A1390 at 1642.228 kb on - strand, within Rru_A1390 at 1642.228 kb on - strand, within Rru_A1390 at 1642.260 kb on + strand, within Rru_A1390 at 1642.260 kb on + strand, within Rru_A1390 at 1642.260 kb on + strand, within Rru_A1390 at 1642.261 kb on - strand, within Rru_A1390 at 1642.261 kb on - strand, within Rru_A1390 at 1642.261 kb on - strand, within Rru_A1390 at 1642.262 kb on + strand, within Rru_A1390 at 1642.263 kb on - strand, within Rru_A1390 at 1642.263 kb on - strand, within Rru_A1390 at 1642.263 kb on - strand, within Rru_A1390 at 1642.263 kb on - strand, within Rru_A1390 at 1642.263 kb on - strand, within Rru_A1390 at 1642.436 kb on + strand at 1642.437 kb on - strand at 1642.437 kb on - strand at 1642.445 kb on + strand at 1642.445 kb on + strand at 1642.966 kb on + strand, within Rru_A1391 at 1642.966 kb on + strand, within Rru_A1391 at 1642.966 kb on + strand, within Rru_A1391 at 1643.324 kb on + strand at 1643.325 kb on - strand at 1643.325 kb on - strand at 1643.347 kb on - strand at 1643.423 kb on + strand at 1643.423 kb on + strand at 1643.423 kb on + strand at 1643.424 kb on - strand at 1643.424 kb on - strand at 1643.424 kb on - strand at 1643.424 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 1,640,937 + -3.6 1,640,938 - -1.2 1,640,949 + -0.2 1,640,949 + -0.1 1,640,950 - -1.2 1,640,950 - +0.8 1,640,958 + -3.8 1,641,058 + +0.4 1,641,059 - -1.7 1,641,059 - -0.6 1,641,290 + Rru_A1389 0.30 -0.3 1,641,290 + Rru_A1389 0.30 -3.7 1,641,290 + Rru_A1389 0.30 +0.4 1,641,291 - Rru_A1389 0.30 -1.3 1,641,291 - Rru_A1389 0.30 +0.4 1,641,533 + Rru_A1389 0.57 -1.8 1,641,534 - Rru_A1389 0.57 +0.1 1,641,599 + Rru_A1389 0.64 +0.1 1,641,599 + Rru_A1389 0.64 +2.9 1,641,600 - Rru_A1389 0.64 -2.9 1,641,600 - Rru_A1389 0.64 +0.4 1,641,606 - Rru_A1389 0.65 -0.1 1,641,662 + Rru_A1389 0.71 +0.3 1,641,740 - Rru_A1389 0.80 +0.4 1,641,740 - Rru_A1389 0.80 -3.5 1,641,900 - -0.1 1,641,923 + -3.8 1,641,924 - +0.3 1,641,924 - -5.4 1,642,034 + Rru_A1390 0.20 -2.5 1,642,035 - Rru_A1390 0.20 +0.4 1,642,035 - Rru_A1390 0.20 +0.1 1,642,035 - Rru_A1390 0.20 +1.1 1,642,035 - Rru_A1390 0.20 -1.3 1,642,086 - Rru_A1390 0.29 -0.3 1,642,131 - Rru_A1390 0.37 -3.7 1,642,191 - Rru_A1390 0.48 -3.8 1,642,227 + Rru_A1390 0.55 +1.3 1,642,227 + Rru_A1390 0.55 -1.4 1,642,227 + Rru_A1390 0.55 -0.5 1,642,228 - Rru_A1390 0.55 +0.3 1,642,228 - Rru_A1390 0.55 -0.1 1,642,260 + Rru_A1390 0.61 -0.5 1,642,260 + Rru_A1390 0.61 -0.1 1,642,260 + Rru_A1390 0.61 +0.4 1,642,261 - Rru_A1390 0.61 +0.4 1,642,261 - Rru_A1390 0.61 +1.1 1,642,261 - Rru_A1390 0.61 +1.0 1,642,262 + Rru_A1390 0.61 +0.2 1,642,263 - Rru_A1390 0.61 -1.9 1,642,263 - Rru_A1390 0.61 +1.0 1,642,263 - Rru_A1390 0.61 -2.7 1,642,263 - Rru_A1390 0.61 -3.0 1,642,263 - Rru_A1390 0.61 -2.5 1,642,436 + +0.8 1,642,437 - -2.5 1,642,437 - -2.3 1,642,445 + -1.9 1,642,445 + -0.6 1,642,966 + Rru_A1391 0.68 -2.2 1,642,966 + Rru_A1391 0.68 +0.4 1,642,966 + Rru_A1391 0.68 -1.3 1,643,324 + -0.2 1,643,325 - -3.5 1,643,325 - -1.7 1,643,347 - +0.1 1,643,423 + +0.2 1,643,423 + +0.6 1,643,423 + -0.1 1,643,424 - +1.9 1,643,424 - -3.4 1,643,424 - +0.5 1,643,424 - -2.8
Or see this region's nucleotide sequence