Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0982 and Rru_A0983 are separated by 8 nucleotides Rru_A0983 and Rru_A0984 are separated by 14 nucleotides
Rru_A0982: Rru_A0982 - ABC transporter component (NCBI), at 1,167,861 to 1,169,486
_A0982
Rru_A0983: Rru_A0983 - Amidase (NCBI), at 1,169,495 to 1,170,952
_A0983
Rru_A0984: Rru_A0984 - Peptidase M20D, amidohydrolase (NCBI), at 1,170,967 to 1,172,160
_A0984
Position (kb)
1169
1170
1171 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1168.626 kb on + strand, within Rru_A0982 at 1168.626 kb on + strand, within Rru_A0982 at 1168.627 kb on - strand, within Rru_A0982 at 1168.628 kb on + strand, within Rru_A0982 at 1168.628 kb on + strand, within Rru_A0982 at 1168.628 kb on + strand, within Rru_A0982 at 1168.629 kb on - strand, within Rru_A0982 at 1168.696 kb on - strand, within Rru_A0982 at 1168.898 kb on + strand, within Rru_A0982 at 1168.898 kb on + strand, within Rru_A0982 at 1168.898 kb on + strand, within Rru_A0982 at 1168.899 kb on - strand, within Rru_A0982 at 1169.367 kb on + strand at 1169.367 kb on + strand at 1169.367 kb on + strand at 1169.367 kb on + strand at 1169.367 kb on + strand at 1169.368 kb on - strand at 1169.368 kb on - strand at 1169.431 kb on - strand at 1169.433 kb on - strand at 1169.433 kb on - strand at 1169.515 kb on + strand at 1169.912 kb on + strand, within Rru_A0983 at 1169.912 kb on + strand, within Rru_A0983 at 1169.912 kb on + strand, within Rru_A0983 at 1169.912 kb on + strand, within Rru_A0983 at 1169.912 kb on + strand, within Rru_A0983 at 1169.913 kb on - strand, within Rru_A0983 at 1169.913 kb on - strand, within Rru_A0983 at 1169.913 kb on - strand, within Rru_A0983 at 1169.913 kb on - strand, within Rru_A0983 at 1169.913 kb on - strand, within Rru_A0983 at 1169.913 kb on - strand, within Rru_A0983 at 1169.914 kb on + strand, within Rru_A0983 at 1169.915 kb on - strand, within Rru_A0983 at 1169.945 kb on + strand, within Rru_A0983 at 1169.946 kb on - strand, within Rru_A0983 at 1170.149 kb on + strand, within Rru_A0983 at 1170.149 kb on + strand, within Rru_A0983 at 1170.150 kb on - strand, within Rru_A0983 at 1170.150 kb on - strand, within Rru_A0983 at 1170.150 kb on - strand, within Rru_A0983 at 1170.494 kb on + strand, within Rru_A0983 at 1170.495 kb on - strand, within Rru_A0983 at 1170.611 kb on + strand, within Rru_A0983 at 1170.734 kb on + strand, within Rru_A0983 at 1170.734 kb on + strand, within Rru_A0983 at 1170.734 kb on + strand, within Rru_A0983 at 1170.735 kb on - strand, within Rru_A0983 at 1170.735 kb on - strand, within Rru_A0983 at 1170.735 kb on - strand, within Rru_A0983 at 1170.890 kb on + strand at 1170.890 kb on + strand at 1170.890 kb on + strand at 1170.891 kb on - strand at 1170.891 kb on - strand at 1170.891 kb on - strand at 1171.027 kb on - strand at 1171.249 kb on + strand, within Rru_A0984 at 1171.249 kb on + strand, within Rru_A0984 at 1171.300 kb on - strand, within Rru_A0984 at 1171.300 kb on - strand, within Rru_A0984 at 1171.431 kb on + strand, within Rru_A0984 at 1171.456 kb on + strand, within Rru_A0984 at 1171.456 kb on + strand, within Rru_A0984 at 1171.457 kb on - strand, within Rru_A0984 at 1171.457 kb on - strand, within Rru_A0984 at 1171.457 kb on - strand, within Rru_A0984 at 1171.491 kb on + strand, within Rru_A0984 at 1171.491 kb on + strand, within Rru_A0984 at 1171.711 kb on + strand, within Rru_A0984 at 1171.711 kb on + strand, within Rru_A0984 at 1171.712 kb on - strand, within Rru_A0984 at 1171.712 kb on - strand, within Rru_A0984 at 1171.714 kb on - strand, within Rru_A0984
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 1,168,626 + Rru_A0982 0.47 -3.0 1,168,626 + Rru_A0982 0.47 -0.8 1,168,627 - Rru_A0982 0.47 -2.8 1,168,628 + Rru_A0982 0.47 +2.0 1,168,628 + Rru_A0982 0.47 -1.2 1,168,628 + Rru_A0982 0.47 -3.1 1,168,629 - Rru_A0982 0.47 +1.0 1,168,696 - Rru_A0982 0.51 +0.9 1,168,898 + Rru_A0982 0.64 +0.8 1,168,898 + Rru_A0982 0.64 +1.7 1,168,898 + Rru_A0982 0.64 -0.7 1,168,899 - Rru_A0982 0.64 +1.4 1,169,367 + +1.3 1,169,367 + -0.5 1,169,367 + -3.6 1,169,367 + -3.4 1,169,367 + -0.6 1,169,368 - +2.1 1,169,368 - -0.3 1,169,431 - -4.1 1,169,433 - +0.3 1,169,433 - -2.5 1,169,515 + +2.0 1,169,912 + Rru_A0983 0.29 +0.5 1,169,912 + Rru_A0983 0.29 +1.1 1,169,912 + Rru_A0983 0.29 -4.1 1,169,912 + Rru_A0983 0.29 -3.5 1,169,912 + Rru_A0983 0.29 +1.0 1,169,913 - Rru_A0983 0.29 -2.0 1,169,913 - Rru_A0983 0.29 +1.3 1,169,913 - Rru_A0983 0.29 -3.1 1,169,913 - Rru_A0983 0.29 -2.1 1,169,913 - Rru_A0983 0.29 +0.3 1,169,913 - Rru_A0983 0.29 -1.5 1,169,914 + Rru_A0983 0.29 -1.6 1,169,915 - Rru_A0983 0.29 -0.1 1,169,945 + Rru_A0983 0.31 +0.7 1,169,946 - Rru_A0983 0.31 -3.9 1,170,149 + Rru_A0983 0.45 -2.2 1,170,149 + Rru_A0983 0.45 -3.0 1,170,150 - Rru_A0983 0.45 +0.4 1,170,150 - Rru_A0983 0.45 -0.4 1,170,150 - Rru_A0983 0.45 -0.1 1,170,494 + Rru_A0983 0.69 +1.4 1,170,495 - Rru_A0983 0.69 +0.2 1,170,611 + Rru_A0983 0.77 -0.9 1,170,734 + Rru_A0983 0.85 +1.7 1,170,734 + Rru_A0983 0.85 +1.4 1,170,734 + Rru_A0983 0.85 -2.0 1,170,735 - Rru_A0983 0.85 -0.5 1,170,735 - Rru_A0983 0.85 -0.5 1,170,735 - Rru_A0983 0.85 -2.2 1,170,890 + -0.1 1,170,890 + -1.5 1,170,890 + +1.3 1,170,891 - +1.2 1,170,891 - -0.8 1,170,891 - -0.9 1,171,027 - -0.5 1,171,249 + Rru_A0984 0.24 -1.1 1,171,249 + Rru_A0984 0.24 -1.7 1,171,300 - Rru_A0984 0.28 -0.9 1,171,300 - Rru_A0984 0.28 +0.1 1,171,431 + Rru_A0984 0.39 +0.3 1,171,456 + Rru_A0984 0.41 -0.4 1,171,456 + Rru_A0984 0.41 +2.2 1,171,457 - Rru_A0984 0.41 +0.6 1,171,457 - Rru_A0984 0.41 -1.1 1,171,457 - Rru_A0984 0.41 -1.5 1,171,491 + Rru_A0984 0.44 +1.1 1,171,491 + Rru_A0984 0.44 -1.3 1,171,711 + Rru_A0984 0.62 +0.0 1,171,711 + Rru_A0984 0.62 -0.8 1,171,712 - Rru_A0984 0.62 -0.3 1,171,712 - Rru_A0984 0.62 +3.1 1,171,714 - Rru_A0984 0.63 +0.0
Or see this region's nucleotide sequence