Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0386 and Rru_A0387 are separated by 75 nucleotides Rru_A0387 and Rru_A0388 are separated by 74 nucleotides Rru_A0388 and Rru_A0389 overlap by 59 nucleotides Rru_A0389 and Rru_A0390 are separated by 188 nucleotides
Rru_A0386: Rru_A0386 - Transcriptional Regulator, ArsR family (NCBI), at 464,330 to 465,022
_A0386
Rru_A0387: Rru_A0387 - Antibiotic biosynthesis monooxygenase (NCBI), at 465,098 to 465,487
_A0387
Rru_A0388: Rru_A0388 - Protein of unknown function DUF6, transmembrane (NCBI), at 465,562 to 466,524
_A0388
Rru_A0389: Rru_A0389 - Antibiotic biosynthesis monooxygenase (NCBI), at 466,466 to 466,756
_A0389
Rru_A0390: Rru_A0390 - hypothetical protein (NCBI), at 466,945 to 467,925
_A0390
Position (kb) 465
466
467 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 464.622 kb on + strand, within Rru_A0386 at 464.623 kb on - strand, within Rru_A0386 at 464.623 kb on - strand, within Rru_A0386 at 464.682 kb on + strand, within Rru_A0386 at 464.682 kb on + strand, within Rru_A0386 at 464.682 kb on + strand, within Rru_A0386 at 464.682 kb on + strand, within Rru_A0386 at 464.682 kb on + strand, within Rru_A0386 at 464.682 kb on + strand, within Rru_A0386 at 464.682 kb on + strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.683 kb on - strand, within Rru_A0386 at 464.811 kb on + strand, within Rru_A0386 at 464.811 kb on + strand, within Rru_A0386 at 464.811 kb on + strand, within Rru_A0386 at 464.812 kb on - strand, within Rru_A0386 at 464.812 kb on - strand, within Rru_A0386 at 464.812 kb on - strand, within Rru_A0386 at 464.812 kb on - strand, within Rru_A0386 at 464.812 kb on - strand, within Rru_A0386 at 464.867 kb on + strand, within Rru_A0386 at 464.867 kb on + strand, within Rru_A0386 at 464.867 kb on + strand, within Rru_A0386 at 464.868 kb on - strand, within Rru_A0386 at 464.868 kb on - strand, within Rru_A0386 at 464.868 kb on - strand, within Rru_A0386 at 464.868 kb on - strand, within Rru_A0386 at 464.894 kb on + strand, within Rru_A0386 at 464.895 kb on - strand, within Rru_A0386 at 464.959 kb on + strand at 464.960 kb on - strand at 464.960 kb on - strand at 464.960 kb on - strand at 465.022 kb on + strand at 465.034 kb on - strand at 465.064 kb on - strand at 465.149 kb on + strand, within Rru_A0387 at 465.149 kb on + strand, within Rru_A0387 at 465.149 kb on + strand, within Rru_A0387 at 465.150 kb on - strand, within Rru_A0387 at 465.150 kb on - strand, within Rru_A0387 at 465.150 kb on - strand, within Rru_A0387 at 465.260 kb on + strand, within Rru_A0387 at 465.354 kb on - strand, within Rru_A0387 at 465.386 kb on + strand, within Rru_A0387 at 465.386 kb on + strand, within Rru_A0387 at 465.387 kb on - strand, within Rru_A0387 at 465.486 kb on - strand at 465.486 kb on - strand at 465.579 kb on - strand at 465.634 kb on + strand at 465.634 kb on + strand at 465.634 kb on + strand at 465.634 kb on + strand at 465.635 kb on - strand at 465.635 kb on - strand at 465.635 kb on - strand at 465.635 kb on - strand at 465.635 kb on - strand at 465.724 kb on - strand, within Rru_A0388 at 465.724 kb on - strand, within Rru_A0388 at 466.064 kb on - strand, within Rru_A0388 at 466.064 kb on - strand, within Rru_A0388 at 466.064 kb on - strand, within Rru_A0388 at 466.267 kb on + strand, within Rru_A0388 at 466.267 kb on + strand, within Rru_A0388 at 466.268 kb on - strand, within Rru_A0388 at 466.268 kb on - strand, within Rru_A0388 at 466.268 kb on - strand, within Rru_A0388 at 466.318 kb on + strand, within Rru_A0388 at 466.319 kb on - strand, within Rru_A0388 at 466.645 kb on - strand, within Rru_A0389 at 466.645 kb on - strand, within Rru_A0389 at 466.645 kb on - strand, within Rru_A0389 at 466.715 kb on - strand, within Rru_A0389 at 466.715 kb on - strand, within Rru_A0389 at 466.750 kb on + strand at 466.750 kb on + strand at 466.750 kb on + strand at 466.864 kb on + strand at 466.864 kb on + strand at 466.865 kb on - strand at 466.865 kb on - strand at 466.984 kb on + strand at 466.984 kb on + strand at 466.984 kb on + strand at 466.985 kb on - strand at 466.985 kb on - strand at 466.985 kb on - strand at 466.985 kb on - strand at 467.017 kb on + strand at 467.017 kb on + strand at 467.018 kb on - strand at 467.018 kb on - strand at 467.029 kb on + strand at 467.029 kb on + strand at 467.029 kb on + strand at 467.030 kb on - strand at 467.030 kb on - strand at 467.049 kb on + strand, within Rru_A0390 at 467.143 kb on + strand, within Rru_A0390 at 467.143 kb on + strand, within Rru_A0390 at 467.143 kb on + strand, within Rru_A0390 at 467.143 kb on + strand, within Rru_A0390 at 467.144 kb on - strand, within Rru_A0390 at 467.144 kb on - strand, within Rru_A0390 at 467.158 kb on + strand, within Rru_A0390 at 467.159 kb on - strand, within Rru_A0390 at 467.200 kb on - strand, within Rru_A0390 at 467.200 kb on - strand, within Rru_A0390 at 467.200 kb on - strand, within Rru_A0390 at 467.200 kb on - strand, within Rru_A0390 at 467.200 kb on - strand, within Rru_A0390 at 467.200 kb on - strand, within Rru_A0390 at 467.200 kb on - strand, within Rru_A0390 at 467.331 kb on + strand, within Rru_A0390 at 467.331 kb on + strand, within Rru_A0390 at 467.335 kb on + strand, within Rru_A0390 at 467.335 kb on + strand, within Rru_A0390 at 467.407 kb on + strand, within Rru_A0390 at 467.407 kb on + strand, within Rru_A0390 at 467.407 kb on + strand, within Rru_A0390 at 467.407 kb on + strand, within Rru_A0390 at 467.407 kb on + strand, within Rru_A0390 at 467.407 kb on + strand, within Rru_A0390 at 467.407 kb on + strand, within Rru_A0390 at 467.407 kb on + strand, within Rru_A0390 at 467.407 kb on + strand, within Rru_A0390 at 467.408 kb on - strand, within Rru_A0390 at 467.408 kb on - strand, within Rru_A0390 at 467.408 kb on - strand, within Rru_A0390 at 467.449 kb on - strand, within Rru_A0390
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 464,622 + Rru_A0386 0.42 +1.3 464,623 - Rru_A0386 0.42 +0.3 464,623 - Rru_A0386 0.42 +0.1 464,682 + Rru_A0386 0.51 -3.1 464,682 + Rru_A0386 0.51 +0.4 464,682 + Rru_A0386 0.51 +0.9 464,682 + Rru_A0386 0.51 +1.3 464,682 + Rru_A0386 0.51 -1.0 464,682 + Rru_A0386 0.51 -3.0 464,682 + Rru_A0386 0.51 -0.3 464,683 - Rru_A0386 0.51 -1.9 464,683 - Rru_A0386 0.51 -0.2 464,683 - Rru_A0386 0.51 +0.6 464,683 - Rru_A0386 0.51 +2.1 464,683 - Rru_A0386 0.51 -2.8 464,683 - Rru_A0386 0.51 +0.7 464,683 - Rru_A0386 0.51 +1.1 464,683 - Rru_A0386 0.51 +0.1 464,683 - Rru_A0386 0.51 -0.8 464,683 - Rru_A0386 0.51 -0.9 464,811 + Rru_A0386 0.69 +1.4 464,811 + Rru_A0386 0.69 +0.4 464,811 + Rru_A0386 0.69 +2.6 464,812 - Rru_A0386 0.70 -0.9 464,812 - Rru_A0386 0.70 -4.1 464,812 - Rru_A0386 0.70 +2.2 464,812 - Rru_A0386 0.70 -2.9 464,812 - Rru_A0386 0.70 +0.6 464,867 + Rru_A0386 0.77 +0.7 464,867 + Rru_A0386 0.77 -0.5 464,867 + Rru_A0386 0.77 +0.7 464,868 - Rru_A0386 0.78 -2.9 464,868 - Rru_A0386 0.78 -1.2 464,868 - Rru_A0386 0.78 +1.1 464,868 - Rru_A0386 0.78 +1.1 464,894 + Rru_A0386 0.81 -2.3 464,895 - Rru_A0386 0.82 -0.7 464,959 + +0.9 464,960 - -2.2 464,960 - +0.9 464,960 - -1.0 465,022 + +0.7 465,034 - +0.7 465,064 - -2.9 465,149 + Rru_A0387 0.13 +0.9 465,149 + Rru_A0387 0.13 -1.5 465,149 + Rru_A0387 0.13 -3.7 465,150 - Rru_A0387 0.13 +2.1 465,150 - Rru_A0387 0.13 -3.7 465,150 - Rru_A0387 0.13 -3.1 465,260 + Rru_A0387 0.42 -3.3 465,354 - Rru_A0387 0.66 +1.5 465,386 + Rru_A0387 0.74 -0.1 465,386 + Rru_A0387 0.74 -3.5 465,387 - Rru_A0387 0.74 +0.1 465,486 - +0.9 465,486 - -0.5 465,579 - +0.6 465,634 + -0.4 465,634 + +0.5 465,634 + +0.5 465,634 + -2.3 465,635 - +1.3 465,635 - -1.3 465,635 - -4.5 465,635 - +0.4 465,635 - -0.2 465,724 - Rru_A0388 0.17 -2.0 465,724 - Rru_A0388 0.17 -0.6 466,064 - Rru_A0388 0.52 +2.7 466,064 - Rru_A0388 0.52 +0.5 466,064 - Rru_A0388 0.52 +2.3 466,267 + Rru_A0388 0.73 +0.4 466,267 + Rru_A0388 0.73 +1.1 466,268 - Rru_A0388 0.73 -0.3 466,268 - Rru_A0388 0.73 -1.7 466,268 - Rru_A0388 0.73 -0.4 466,318 + Rru_A0388 0.79 +0.2 466,319 - Rru_A0388 0.79 +0.5 466,645 - Rru_A0389 0.62 +1.5 466,645 - Rru_A0389 0.62 -2.5 466,645 - Rru_A0389 0.62 +0.2 466,715 - Rru_A0389 0.86 -3.7 466,715 - Rru_A0389 0.86 +0.3 466,750 + +1.1 466,750 + -1.5 466,750 + -1.4 466,864 + +0.6 466,864 + +0.4 466,865 - -0.2 466,865 - +0.0 466,984 + +0.9 466,984 + +1.8 466,984 + -3.8 466,985 - -1.7 466,985 - -0.1 466,985 - +1.5 466,985 - -0.2 467,017 + +0.8 467,017 + -0.2 467,018 - +1.2 467,018 - -0.5 467,029 + +0.7 467,029 + +0.7 467,029 + +1.1 467,030 - -2.0 467,030 - -0.7 467,049 + Rru_A0390 0.11 +0.5 467,143 + Rru_A0390 0.20 +1.1 467,143 + Rru_A0390 0.20 -0.3 467,143 + Rru_A0390 0.20 -0.6 467,143 + Rru_A0390 0.20 +0.8 467,144 - Rru_A0390 0.20 +1.4 467,144 - Rru_A0390 0.20 +0.3 467,158 + Rru_A0390 0.22 -0.7 467,159 - Rru_A0390 0.22 +0.6 467,200 - Rru_A0390 0.26 -3.2 467,200 - Rru_A0390 0.26 +0.8 467,200 - Rru_A0390 0.26 +0.8 467,200 - Rru_A0390 0.26 +1.3 467,200 - Rru_A0390 0.26 +0.7 467,200 - Rru_A0390 0.26 -1.7 467,200 - Rru_A0390 0.26 -1.3 467,331 + Rru_A0390 0.39 -0.1 467,331 + Rru_A0390 0.39 +2.3 467,335 + Rru_A0390 0.40 +1.7 467,335 + Rru_A0390 0.40 -1.0 467,407 + Rru_A0390 0.47 +1.1 467,407 + Rru_A0390 0.47 +1.2 467,407 + Rru_A0390 0.47 +0.2 467,407 + Rru_A0390 0.47 -4.0 467,407 + Rru_A0390 0.47 +1.3 467,407 + Rru_A0390 0.47 -0.7 467,407 + Rru_A0390 0.47 -0.2 467,407 + Rru_A0390 0.47 +1.5 467,407 + Rru_A0390 0.47 -1.0 467,408 - Rru_A0390 0.47 +0.8 467,408 - Rru_A0390 0.47 -0.8 467,408 - Rru_A0390 0.47 +1.1 467,449 - Rru_A0390 0.51 +1.1
Or see this region's nucleotide sequence