Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0367 and Rru_A0368 are separated by 32 nucleotides Rru_A0368 and Rru_A0369 are separated by 27 nucleotides Rru_A0369 and Rru_A0370 are separated by 5 nucleotides Rru_A0370 and Rru_A0371 overlap by 4 nucleotides
Rru_A0367: Rru_A0367 - NUDIX hydrolase (NCBI), at 441,764 to 442,495
_A0367
Rru_A0368: Rru_A0368 - Protein of unknown function DUF1285 (NCBI), at 442,528 to 443,127
_A0368
Rru_A0369: Rru_A0369 - ATPase associated with various cellular activities, AAA_3 (NCBI), at 443,155 to 444,165
_A0369
Rru_A0370: Rru_A0370 - Protein of unknown function DUF58 (NCBI), at 444,171 to 445,109
_A0370
Rru_A0371: Rru_A0371 - Double-transmembrane region-like (NCBI), at 445,106 to 447,889
_A0371
Position (kb)
443
444
445 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 442.388 kb on + strand, within Rru_A0367 at 442.389 kb on - strand, within Rru_A0367 at 442.529 kb on + strand at 442.529 kb on + strand at 443.193 kb on - strand at 443.193 kb on - strand at 443.426 kb on + strand, within Rru_A0369 at 443.427 kb on - strand, within Rru_A0369 at 443.618 kb on - strand, within Rru_A0369 at 443.648 kb on + strand, within Rru_A0369 at 443.648 kb on + strand, within Rru_A0369 at 443.649 kb on - strand, within Rru_A0369 at 443.683 kb on + strand, within Rru_A0369 at 443.683 kb on + strand, within Rru_A0369 at 443.683 kb on + strand, within Rru_A0369 at 443.683 kb on + strand, within Rru_A0369 at 443.683 kb on + strand, within Rru_A0369 at 443.684 kb on - strand, within Rru_A0369 at 443.684 kb on - strand, within Rru_A0369 at 443.684 kb on - strand, within Rru_A0369 at 444.435 kb on + strand, within Rru_A0370 at 444.495 kb on + strand, within Rru_A0370 at 444.496 kb on - strand, within Rru_A0370 at 444.496 kb on - strand, within Rru_A0370 at 444.496 kb on - strand, within Rru_A0370 at 445.123 kb on + strand at 445.123 kb on + strand at 445.139 kb on + strand at 445.139 kb on + strand at 445.139 kb on + strand at 445.140 kb on - strand at 445.140 kb on - strand at 445.140 kb on - strand at 445.140 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 442,388 + Rru_A0367 0.85 -1.2 442,389 - Rru_A0367 0.85 -1.6 442,529 + +1.2 442,529 + +0.6 443,193 - +1.9 443,193 - -2.0 443,426 + Rru_A0369 0.27 -0.9 443,427 - Rru_A0369 0.27 -3.6 443,618 - Rru_A0369 0.46 -2.3 443,648 + Rru_A0369 0.49 -0.6 443,648 + Rru_A0369 0.49 -3.7 443,649 - Rru_A0369 0.49 -1.8 443,683 + Rru_A0369 0.52 +0.1 443,683 + Rru_A0369 0.52 -2.4 443,683 + Rru_A0369 0.52 -2.2 443,683 + Rru_A0369 0.52 -3.9 443,683 + Rru_A0369 0.52 -1.1 443,684 - Rru_A0369 0.52 -3.5 443,684 - Rru_A0369 0.52 +1.5 443,684 - Rru_A0369 0.52 -2.7 444,435 + Rru_A0370 0.28 -0.5 444,495 + Rru_A0370 0.35 -2.5 444,496 - Rru_A0370 0.35 -0.3 444,496 - Rru_A0370 0.35 +0.8 444,496 - Rru_A0370 0.35 -1.3 445,123 + -2.2 445,123 + +1.3 445,139 + -2.3 445,139 + -2.9 445,139 + +0.9 445,140 - +1.7 445,140 - -3.2 445,140 - +1.7 445,140 - +0.7
Or see this region's nucleotide sequence