Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0305 and Rru_A0306 overlap by 4 nucleotides Rru_A0306 and Rru_A0307 overlap by 4 nucleotides Rru_A0307 and Rru_A0308 are separated by 0 nucleotides
Rru_A0305: Rru_A0305 - Hydrogenase expression/formation protein HypE (NCBI), at 372,015 to 373,040
_A0305
Rru_A0306: Rru_A0306 - Hydrogenase formation HypD protein (NCBI), at 373,037 to 374,197
_A0306
Rru_A0307: Rru_A0307 - Hydrogenase expression/formation protein (HUPF/HYPC) (NCBI), at 374,194 to 374,424
_A0307
Rru_A0308: Rru_A0308 - Hydrogenase maturation protein HypF (NCBI), at 374,425 to 376,731
_A0308
Position (kb)
373
374
375 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 372.522 kb on + strand, within Rru_A0305 at 372.523 kb on - strand, within Rru_A0305 at 372.523 kb on - strand, within Rru_A0305 at 372.523 kb on - strand, within Rru_A0305 at 372.570 kb on + strand, within Rru_A0305 at 372.741 kb on - strand, within Rru_A0305 at 372.741 kb on - strand, within Rru_A0305 at 372.757 kb on + strand, within Rru_A0305 at 372.757 kb on + strand, within Rru_A0305 at 372.757 kb on + strand, within Rru_A0305 at 372.758 kb on - strand, within Rru_A0305 at 372.758 kb on - strand, within Rru_A0305 at 373.084 kb on - strand at 373.084 kb on - strand at 373.089 kb on + strand at 373.090 kb on - strand at 373.090 kb on - strand at 373.145 kb on - strand at 373.284 kb on + strand, within Rru_A0306 at 373.285 kb on - strand, within Rru_A0306 at 373.370 kb on - strand, within Rru_A0306 at 373.418 kb on - strand, within Rru_A0306 at 373.917 kb on + strand, within Rru_A0306 at 373.917 kb on + strand, within Rru_A0306 at 373.917 kb on + strand, within Rru_A0306 at 373.918 kb on - strand, within Rru_A0306 at 373.918 kb on - strand, within Rru_A0306 at 373.918 kb on - strand, within Rru_A0306 at 373.918 kb on - strand, within Rru_A0306 at 374.326 kb on + strand, within Rru_A0307 at 374.326 kb on + strand, within Rru_A0307 at 374.326 kb on + strand, within Rru_A0307 at 374.327 kb on - strand, within Rru_A0307 at 374.327 kb on - strand, within Rru_A0307 at 374.426 kb on + strand at 374.426 kb on + strand at 374.426 kb on + strand at 374.426 kb on + strand at 374.427 kb on - strand at 374.427 kb on - strand at 374.427 kb on - strand at 374.427 kb on - strand at 374.456 kb on - strand at 374.468 kb on + strand at 374.469 kb on - strand at 374.556 kb on + strand at 374.557 kb on - strand at 374.829 kb on - strand, within Rru_A0308 at 374.924 kb on - strand, within Rru_A0308 at 375.186 kb on + strand, within Rru_A0308
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 372,522 + Rru_A0305 0.49 +2.3 372,523 - Rru_A0305 0.50 -0.1 372,523 - Rru_A0305 0.50 -1.6 372,523 - Rru_A0305 0.50 -1.1 372,570 + Rru_A0305 0.54 +0.7 372,741 - Rru_A0305 0.71 -2.3 372,741 - Rru_A0305 0.71 +0.9 372,757 + Rru_A0305 0.72 +1.1 372,757 + Rru_A0305 0.72 -2.8 372,757 + Rru_A0305 0.72 -3.5 372,758 - Rru_A0305 0.72 -3.5 372,758 - Rru_A0305 0.72 -2.4 373,084 - -3.7 373,084 - -2.7 373,089 + +0.6 373,090 - +0.8 373,090 - +1.9 373,145 - +1.0 373,284 + Rru_A0306 0.21 +0.4 373,285 - Rru_A0306 0.21 +1.4 373,370 - Rru_A0306 0.29 +1.4 373,418 - Rru_A0306 0.33 +1.3 373,917 + Rru_A0306 0.76 +1.8 373,917 + Rru_A0306 0.76 -0.4 373,917 + Rru_A0306 0.76 +0.2 373,918 - Rru_A0306 0.76 -1.7 373,918 - Rru_A0306 0.76 +0.7 373,918 - Rru_A0306 0.76 +0.6 373,918 - Rru_A0306 0.76 +0.7 374,326 + Rru_A0307 0.57 -1.1 374,326 + Rru_A0307 0.57 +0.3 374,326 + Rru_A0307 0.57 +1.4 374,327 - Rru_A0307 0.58 +1.6 374,327 - Rru_A0307 0.58 -0.2 374,426 + -1.0 374,426 + +1.1 374,426 + +0.6 374,426 + +0.4 374,427 - +0.7 374,427 - +2.4 374,427 - -2.8 374,427 - -1.6 374,456 - +0.2 374,468 + +0.2 374,469 - +0.7 374,556 + +0.7 374,557 - -1.9 374,829 - Rru_A0308 0.18 +0.8 374,924 - Rru_A0308 0.22 -0.3 375,186 + Rru_A0308 0.33 -0.5
Or see this region's nucleotide sequence