Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0243 and Rru_A0244 are separated by 191 nucleotides Rru_A0244 and Rru_A0245 are separated by 166 nucleotides Rru_A0245 and Rru_A0246 overlap by 4 nucleotides
Rru_A0243: Rru_A0243 - tRNA/rRNA cytosine-C5-methylase (NCBI), at 301,475 to 302,839
_A0243
Rru_A0244: Rru_A0244 - IMP dehydrogenase (NCBI), at 303,031 to 304,494
_A0244
Rru_A0245: Rru_A0245 - Ribosomal RNA methyltransferase RrmJ/FtsJ (NCBI), at 304,661 to 305,452
_A0245
Rru_A0246: Rru_A0246 - Ppx/GppA phosphatase (NCBI), at 305,449 to 306,648
_A0246
Position (kb) 303
304
305 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 302.033 kb on - strand, within Rru_A0243 at 302.471 kb on + strand, within Rru_A0243 at 302.471 kb on + strand, within Rru_A0243 at 303.021 kb on + strand at 303.027 kb on + strand at 303.027 kb on + strand at 303.027 kb on + strand at 303.028 kb on - strand at 303.028 kb on - strand at 303.028 kb on - strand at 303.028 kb on - strand at 303.028 kb on - strand at 303.032 kb on + strand at 303.032 kb on + strand at 303.032 kb on + strand at 303.033 kb on - strand at 303.033 kb on - strand at 303.033 kb on - strand at 304.512 kb on - strand at 304.662 kb on + strand at 304.663 kb on - strand at 304.663 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 302,033 - Rru_A0243 0.41 +0.6 302,471 + Rru_A0243 0.73 -2.9 302,471 + Rru_A0243 0.73 -1.4 303,021 + +0.7 303,027 + +1.3 303,027 + +1.6 303,027 + -0.4 303,028 - +0.1 303,028 - +0.1 303,028 - -1.1 303,028 - -1.7 303,028 - +0.5 303,032 + +0.5 303,032 + +0.6 303,032 + +0.3 303,033 - -0.8 303,033 - +0.1 303,033 - -1.1 304,512 - +1.6 304,662 + -0.0 304,663 - -1.0 304,663 - +1.0
Or see this region's nucleotide sequence