Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0209 and Rru_A0210 are separated by 217 nucleotides Rru_A0210 and Rru_A0211 are separated by 147 nucleotides
Rru_A0209: Rru_A0209 - Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B (NCBI), at 261,647 to 262,486
_A0209
Rru_A0210: Rru_A0210 - Ribosomal protein L28 (NCBI), at 262,704 to 263,003
_A0210
Rru_A0211: Rru_A0211 - hypothetical protein (NCBI), at 263,151 to 263,912
_A0211
Position (kb)
262
263
264 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 261.792 kb on + strand, within Rru_A0209 at 261.792 kb on + strand, within Rru_A0209 at 261.792 kb on + strand, within Rru_A0209 at 261.792 kb on + strand, within Rru_A0209 at 261.792 kb on + strand, within Rru_A0209 at 261.793 kb on - strand, within Rru_A0209 at 261.793 kb on - strand, within Rru_A0209 at 261.793 kb on - strand, within Rru_A0209 at 261.793 kb on - strand, within Rru_A0209 at 261.974 kb on - strand, within Rru_A0209 at 262.404 kb on + strand at 262.405 kb on - strand at 262.405 kb on - strand at 262.506 kb on + strand at 262.507 kb on - strand at 262.580 kb on - strand at 262.580 kb on - strand at 262.580 kb on - strand at 263.061 kb on - strand at 263.087 kb on - strand at 263.431 kb on + strand, within Rru_A0211 at 263.432 kb on - strand, within Rru_A0211 at 263.432 kb on - strand, within Rru_A0211 at 263.702 kb on + strand, within Rru_A0211 at 263.702 kb on + strand, within Rru_A0211 at 263.702 kb on + strand, within Rru_A0211 at 263.703 kb on - strand, within Rru_A0211 at 263.703 kb on - strand, within Rru_A0211 at 263.973 kb on + strand at 263.980 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 261,792 + Rru_A0209 0.17 -0.7 261,792 + Rru_A0209 0.17 -0.3 261,792 + Rru_A0209 0.17 -3.0 261,792 + Rru_A0209 0.17 +1.5 261,792 + Rru_A0209 0.17 +0.3 261,793 - Rru_A0209 0.17 +0.2 261,793 - Rru_A0209 0.17 -0.8 261,793 - Rru_A0209 0.17 -0.5 261,793 - Rru_A0209 0.17 +0.9 261,974 - Rru_A0209 0.39 -3.7 262,404 + +0.6 262,405 - -0.8 262,405 - -0.9 262,506 + -0.5 262,507 - -3.2 262,580 - +0.4 262,580 - -1.8 262,580 - -0.5 263,061 - -2.8 263,087 - -0.5 263,431 + Rru_A0211 0.37 +0.2 263,432 - Rru_A0211 0.37 -1.7 263,432 - Rru_A0211 0.37 -0.3 263,702 + Rru_A0211 0.72 +0.1 263,702 + Rru_A0211 0.72 +0.1 263,702 + Rru_A0211 0.72 +0.6 263,703 - Rru_A0211 0.72 +0.4 263,703 - Rru_A0211 0.72 -3.1 263,973 + -0.1 263,980 - +0.6
Or see this region's nucleotide sequence