Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0172 and Rru_A0173 are separated by 2 nucleotides Rru_A0173 and Rru_A0174 overlap by 4 nucleotides Rru_A0174 and Rru_A0175 are separated by 288 nucleotides
Rru_A0172: Rru_A0172 - CRISPR-associated protein, CT1975 (NCBI), at 202,682 to 203,761
_A0172
Rru_A0173: Rru_A0173 - hypothetical protein (NCBI), at 203,764 to 204,366
_A0173
Rru_A0174: Rru_A0174 - hypothetical protein (NCBI), at 204,363 to 206,030
_A0174
Rru_A0175: Rru_A0175 - CRISPR-associated helicase Cas3, core (NCBI), at 206,319 to 209,081
_A0175
Position (kb)
204
205
206
207 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 203.777 kb on - strand at 203.855 kb on + strand, within Rru_A0173 at 203.855 kb on + strand, within Rru_A0173 at 203.971 kb on + strand, within Rru_A0173 at 203.971 kb on + strand, within Rru_A0173 at 203.971 kb on + strand, within Rru_A0173 at 203.972 kb on - strand, within Rru_A0173 at 204.525 kb on - strand at 204.568 kb on - strand, within Rru_A0174 at 204.568 kb on - strand, within Rru_A0174 at 204.774 kb on + strand, within Rru_A0174 at 204.850 kb on + strand, within Rru_A0174 at 204.850 kb on + strand, within Rru_A0174 at 205.010 kb on - strand, within Rru_A0174 at 205.010 kb on - strand, within Rru_A0174 at 205.036 kb on + strand, within Rru_A0174 at 205.036 kb on + strand, within Rru_A0174 at 205.036 kb on + strand, within Rru_A0174 at 205.037 kb on - strand, within Rru_A0174 at 205.037 kb on - strand, within Rru_A0174 at 205.037 kb on - strand, within Rru_A0174 at 205.126 kb on + strand, within Rru_A0174 at 205.138 kb on + strand, within Rru_A0174 at 205.138 kb on + strand, within Rru_A0174 at 205.138 kb on + strand, within Rru_A0174 at 205.139 kb on - strand, within Rru_A0174 at 205.139 kb on - strand, within Rru_A0174 at 205.139 kb on - strand, within Rru_A0174 at 205.139 kb on - strand, within Rru_A0174 at 205.543 kb on + strand, within Rru_A0174 at 205.543 kb on + strand, within Rru_A0174 at 205.544 kb on - strand, within Rru_A0174 at 205.544 kb on - strand, within Rru_A0174 at 205.576 kb on + strand, within Rru_A0174 at 205.665 kb on + strand, within Rru_A0174 at 205.665 kb on + strand, within Rru_A0174 at 205.666 kb on - strand, within Rru_A0174 at 205.666 kb on - strand, within Rru_A0174 at 205.666 kb on - strand, within Rru_A0174 at 205.666 kb on - strand, within Rru_A0174 at 205.709 kb on + strand, within Rru_A0174 at 205.709 kb on + strand, within Rru_A0174 at 205.710 kb on - strand, within Rru_A0174 at 205.852 kb on + strand, within Rru_A0174 at 205.853 kb on - strand, within Rru_A0174 at 205.866 kb on + strand at 205.947 kb on - strand at 206.021 kb on + strand at 206.021 kb on + strand at 206.022 kb on - strand at 206.022 kb on - strand at 206.022 kb on - strand at 206.022 kb on - strand at 206.057 kb on - strand at 206.057 kb on - strand at 206.177 kb on + strand at 206.178 kb on - strand at 206.180 kb on + strand at 206.180 kb on + strand at 206.180 kb on + strand at 206.181 kb on - strand at 206.196 kb on - strand at 206.196 kb on - strand at 206.237 kb on + strand at 206.238 kb on - strand at 206.240 kb on + strand at 206.241 kb on - strand at 206.246 kb on + strand at 206.294 kb on + strand at 206.309 kb on + strand at 206.310 kb on - strand at 206.310 kb on - strand at 206.362 kb on + strand at 206.363 kb on - strand at 206.363 kb on - strand at 206.547 kb on + strand at 206.711 kb on - strand, within Rru_A0175 at 206.740 kb on + strand, within Rru_A0175 at 206.740 kb on + strand, within Rru_A0175 at 206.741 kb on - strand, within Rru_A0175 at 206.741 kb on - strand, within Rru_A0175 at 206.844 kb on + strand, within Rru_A0175 at 206.845 kb on - strand, within Rru_A0175 at 206.845 kb on - strand, within Rru_A0175 at 206.966 kb on - strand, within Rru_A0175 at 206.966 kb on - strand, within Rru_A0175
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 203,777 - +0.4 203,855 + Rru_A0173 0.15 -0.9 203,855 + Rru_A0173 0.15 +0.5 203,971 + Rru_A0173 0.34 +1.1 203,971 + Rru_A0173 0.34 +1.0 203,971 + Rru_A0173 0.34 -1.9 203,972 - Rru_A0173 0.34 -0.9 204,525 - -0.9 204,568 - Rru_A0174 0.12 +2.6 204,568 - Rru_A0174 0.12 -2.0 204,774 + Rru_A0174 0.25 +0.9 204,850 + Rru_A0174 0.29 -3.9 204,850 + Rru_A0174 0.29 -0.0 205,010 - Rru_A0174 0.39 +0.0 205,010 - Rru_A0174 0.39 +0.2 205,036 + Rru_A0174 0.40 -2.5 205,036 + Rru_A0174 0.40 -0.5 205,036 + Rru_A0174 0.40 -3.5 205,037 - Rru_A0174 0.40 +0.7 205,037 - Rru_A0174 0.40 -1.4 205,037 - Rru_A0174 0.40 -1.4 205,126 + Rru_A0174 0.46 -0.5 205,138 + Rru_A0174 0.46 -1.1 205,138 + Rru_A0174 0.46 +1.0 205,138 + Rru_A0174 0.46 -0.5 205,139 - Rru_A0174 0.47 -2.6 205,139 - Rru_A0174 0.47 -0.9 205,139 - Rru_A0174 0.47 -0.2 205,139 - Rru_A0174 0.47 +1.1 205,543 + Rru_A0174 0.71 -1.0 205,543 + Rru_A0174 0.71 +0.6 205,544 - Rru_A0174 0.71 +0.9 205,544 - Rru_A0174 0.71 +0.8 205,576 + Rru_A0174 0.73 +2.4 205,665 + Rru_A0174 0.78 +1.8 205,665 + Rru_A0174 0.78 +1.4 205,666 - Rru_A0174 0.78 +0.6 205,666 - Rru_A0174 0.78 -0.1 205,666 - Rru_A0174 0.78 +1.2 205,666 - Rru_A0174 0.78 +0.3 205,709 + Rru_A0174 0.81 -1.0 205,709 + Rru_A0174 0.81 +1.4 205,710 - Rru_A0174 0.81 -1.4 205,852 + Rru_A0174 0.89 -0.5 205,853 - Rru_A0174 0.89 -2.2 205,866 + -1.0 205,947 - -0.2 206,021 + -0.9 206,021 + +1.5 206,022 - +0.6 206,022 - +1.9 206,022 - -0.1 206,022 - -0.3 206,057 - -1.3 206,057 - -0.0 206,177 + +1.5 206,178 - -0.2 206,180 + +0.4 206,180 + -2.0 206,180 + -3.2 206,181 - -1.3 206,196 - +0.1 206,196 - +2.3 206,237 + -3.3 206,238 - -2.5 206,240 + -2.9 206,241 - -1.4 206,246 + +1.1 206,294 + +0.3 206,309 + -2.1 206,310 - +0.8 206,310 - +2.7 206,362 + +0.4 206,363 - -1.5 206,363 - -2.0 206,547 + +0.5 206,711 - Rru_A0175 0.14 +1.0 206,740 + Rru_A0175 0.15 +0.4 206,740 + Rru_A0175 0.15 -0.4 206,741 - Rru_A0175 0.15 -0.1 206,741 - Rru_A0175 0.15 -1.1 206,844 + Rru_A0175 0.19 -2.9 206,845 - Rru_A0175 0.19 -1.6 206,845 - Rru_A0175 0.19 +1.4 206,966 - Rru_A0175 0.23 -3.9 206,966 - Rru_A0175 0.23 -0.7
Or see this region's nucleotide sequence