Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0128 and Rru_A0129 are separated by 100 nucleotides Rru_A0129 and Rru_AR0003 are separated by 225 nucleotides Rru_AR0003 and Rru_A0130 are separated by 136 nucleotides
Rru_A0128: Rru_A0128 - Na+/H+ antiporter NhaA (NCBI), at 148,621 to 149,820
_A0128
Rru_A0129: Rru_A0129 - hypothetical protein (NCBI), at 149,921 to 151,015
_A0129
Rru_AR0003: Rru_AR0003 - tRNA-Arg (NCBI), at 151,241 to 151,317
_AR0003
Rru_A0130: Rru_A0130 - chemotaxis sensory transducer (NCBI), at 151,454 to 152,929
_A0130
Position (kb) 149
150
151
152 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 149.022 kb on - strand, within Rru_A0128 at 149.022 kb on - strand, within Rru_A0128 at 149.227 kb on - strand, within Rru_A0128 at 149.227 kb on - strand, within Rru_A0128 at 149.295 kb on - strand, within Rru_A0128 at 149.484 kb on - strand, within Rru_A0128 at 149.484 kb on - strand, within Rru_A0128 at 149.484 kb on - strand, within Rru_A0128 at 149.843 kb on + strand at 149.873 kb on - strand at 149.873 kb on - strand at 149.873 kb on - strand at 149.884 kb on - strand at 149.884 kb on - strand at 149.942 kb on + strand at 149.943 kb on - strand at 150.336 kb on - strand, within Rru_A0129 at 150.363 kb on + strand, within Rru_A0129 at 150.363 kb on + strand, within Rru_A0129 at 150.843 kb on + strand, within Rru_A0129 at 151.175 kb on + strand at 151.220 kb on + strand at 151.456 kb on - strand at 151.456 kb on - strand at 151.460 kb on + strand at 151.460 kb on + strand at 151.549 kb on + strand at 151.549 kb on + strand at 151.550 kb on - strand at 151.550 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 149,022 - Rru_A0128 0.33 -3.1 149,022 - Rru_A0128 0.33 +0.4 149,227 - Rru_A0128 0.51 -2.2 149,227 - Rru_A0128 0.51 -1.5 149,295 - Rru_A0128 0.56 -1.7 149,484 - Rru_A0128 0.72 -2.3 149,484 - Rru_A0128 0.72 -1.0 149,484 - Rru_A0128 0.72 +1.9 149,843 + +2.0 149,873 - -0.3 149,873 - -1.0 149,873 - -2.2 149,884 - -2.2 149,884 - +1.0 149,942 + -1.7 149,943 - -1.9 150,336 - Rru_A0129 0.38 -1.2 150,363 + Rru_A0129 0.40 -4.5 150,363 + Rru_A0129 0.40 +0.3 150,843 + Rru_A0129 0.84 -0.6 151,175 + +1.1 151,220 + -0.9 151,456 - +0.3 151,456 - -0.1 151,460 + +2.1 151,460 + -0.9 151,549 + +0.6 151,549 + +0.6 151,550 - +0.4 151,550 - -2.7
Or see this region's nucleotide sequence