Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0111 and Rru_A0112 overlap by 4 nucleotides Rru_A0112 and Rru_A0113 are separated by 85 nucleotides Rru_A0113 and Rru_A0114 overlap by 1 nucleotides
Rru_A0111: Rru_A0111 - hypothetical protein (NCBI), at 130,112 to 130,702
_A0111
Rru_A0112: Rru_A0112 - hypothetical protein (NCBI), at 130,699 to 131,421
_A0112
Rru_A0113: Rru_A0113 - type II secretion system protein E (NCBI), at 131,507 to 133,573
_A0113
Rru_A0114: Rru_A0114 - type II secretion system protein (NCBI), at 133,573 to 134,808
_A0114
Position (kb) 131
132
133
134 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 131.211 kb on + strand, within Rru_A0112 at 131.211 kb on + strand, within Rru_A0112 at 131.212 kb on - strand, within Rru_A0112 at 131.276 kb on - strand, within Rru_A0112 at 131.302 kb on + strand, within Rru_A0112 at 131.302 kb on + strand, within Rru_A0112 at 131.303 kb on - strand, within Rru_A0112 at 131.303 kb on - strand, within Rru_A0112 at 131.303 kb on - strand, within Rru_A0112 at 131.304 kb on + strand, within Rru_A0112 at 131.305 kb on - strand, within Rru_A0112 at 131.305 kb on - strand, within Rru_A0112 at 131.305 kb on - strand, within Rru_A0112 at 131.372 kb on - strand at 132.240 kb on - strand, within Rru_A0113 at 132.311 kb on + strand, within Rru_A0113 at 132.311 kb on + strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.312 kb on - strand, within Rru_A0113 at 132.398 kb on - strand, within Rru_A0113 at 132.398 kb on - strand, within Rru_A0113 at 132.550 kb on + strand, within Rru_A0113 at 132.557 kb on + strand, within Rru_A0113 at 132.869 kb on + strand, within Rru_A0113 at 132.870 kb on - strand, within Rru_A0113 at 132.886 kb on + strand, within Rru_A0113 at 132.886 kb on + strand, within Rru_A0113 at 132.887 kb on - strand, within Rru_A0113 at 132.935 kb on + strand, within Rru_A0113 at 133.109 kb on + strand, within Rru_A0113 at 133.110 kb on - strand, within Rru_A0113 at 133.359 kb on - strand, within Rru_A0113 at 133.396 kb on + strand at 133.396 kb on + strand at 133.583 kb on - strand at 133.588 kb on + strand at 134.162 kb on + strand, within Rru_A0114
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 131,211 + Rru_A0112 0.71 -2.4 131,211 + Rru_A0112 0.71 +0.0 131,212 - Rru_A0112 0.71 -1.4 131,276 - Rru_A0112 0.80 -3.0 131,302 + Rru_A0112 0.83 +1.2 131,302 + Rru_A0112 0.83 -3.4 131,303 - Rru_A0112 0.84 -1.5 131,303 - Rru_A0112 0.84 -0.3 131,303 - Rru_A0112 0.84 -1.2 131,304 + Rru_A0112 0.84 +0.2 131,305 - Rru_A0112 0.84 +0.7 131,305 - Rru_A0112 0.84 +0.4 131,305 - Rru_A0112 0.84 +0.1 131,372 - +0.1 132,240 - Rru_A0113 0.35 +0.1 132,311 + Rru_A0113 0.39 +0.9 132,311 + Rru_A0113 0.39 +1.3 132,312 - Rru_A0113 0.39 -1.1 132,312 - Rru_A0113 0.39 +0.9 132,312 - Rru_A0113 0.39 +0.3 132,312 - Rru_A0113 0.39 -0.3 132,312 - Rru_A0113 0.39 +0.5 132,312 - Rru_A0113 0.39 +0.1 132,398 - Rru_A0113 0.43 +1.5 132,398 - Rru_A0113 0.43 -0.1 132,550 + Rru_A0113 0.50 +1.1 132,557 + Rru_A0113 0.51 -0.4 132,869 + Rru_A0113 0.66 -2.4 132,870 - Rru_A0113 0.66 +0.5 132,886 + Rru_A0113 0.67 -2.8 132,886 + Rru_A0113 0.67 +1.0 132,887 - Rru_A0113 0.67 +0.5 132,935 + Rru_A0113 0.69 -1.8 133,109 + Rru_A0113 0.78 -0.3 133,110 - Rru_A0113 0.78 -0.4 133,359 - Rru_A0113 0.90 +1.5 133,396 + -2.7 133,396 + -0.6 133,583 - -0.2 133,588 + -3.8 134,162 + Rru_A0114 0.48 -2.1
Or see this region's nucleotide sequence