Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0104 and Rru_A0105 are separated by 8 nucleotides Rru_A0105 and Rru_A0106 are separated by 94 nucleotides Rru_A0106 and Rru_A0107 overlap by 4 nucleotides
Rru_A0104: Rru_A0104 - Alpha-2-macroglobulin-like protein (NCBI), at 117,743 to 123,022
_A0104
Rru_A0105: Rru_A0105 - Penicillin-binding protein 1C (NCBI), at 123,031 to 125,151
_A0105
Rru_A0106: Rru_A0106 - TPR repeat, Tetratricopeptide TPR_4 (NCBI), at 125,246 to 126,043
_A0106
Rru_A0107: Rru_A0107 - MSHA biogenesis protein MshM (NCBI), at 126,040 to 126,951
_A0107
Position (kb)
123
124
125
126 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 122.051 kb on - strand, within Rru_A0104 at 122.054 kb on + strand, within Rru_A0104 at 122.054 kb on + strand, within Rru_A0104 at 122.054 kb on + strand, within Rru_A0104 at 122.054 kb on + strand, within Rru_A0104 at 122.055 kb on - strand, within Rru_A0104 at 122.055 kb on - strand, within Rru_A0104 at 122.055 kb on - strand, within Rru_A0104 at 122.055 kb on - strand, within Rru_A0104 at 122.055 kb on - strand, within Rru_A0104 at 122.202 kb on - strand, within Rru_A0104 at 122.202 kb on - strand, within Rru_A0104 at 122.849 kb on + strand at 122.849 kb on + strand at 122.849 kb on + strand at 122.849 kb on + strand at 122.850 kb on - strand at 122.850 kb on - strand at 122.900 kb on + strand at 122.901 kb on - strand at 122.901 kb on - strand at 122.901 kb on - strand at 122.959 kb on + strand at 122.960 kb on - strand at 123.262 kb on + strand, within Rru_A0105 at 123.262 kb on + strand, within Rru_A0105 at 123.262 kb on + strand, within Rru_A0105 at 123.263 kb on - strand, within Rru_A0105 at 123.517 kb on + strand, within Rru_A0105 at 123.518 kb on - strand, within Rru_A0105 at 124.007 kb on - strand, within Rru_A0105 at 124.087 kb on + strand, within Rru_A0105 at 124.111 kb on - strand, within Rru_A0105 at 124.162 kb on + strand, within Rru_A0105 at 124.162 kb on + strand, within Rru_A0105 at 124.600 kb on + strand, within Rru_A0105 at 124.601 kb on - strand, within Rru_A0105 at 125.161 kb on - strand at 125.177 kb on - strand at 125.625 kb on - strand, within Rru_A0106 at 125.788 kb on - strand, within Rru_A0106 at 125.788 kb on - strand, within Rru_A0106 at 125.788 kb on - strand, within Rru_A0106
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 122,051 - Rru_A0104 0.82 -1.1 122,054 + Rru_A0104 0.82 +0.1 122,054 + Rru_A0104 0.82 +0.6 122,054 + Rru_A0104 0.82 +0.3 122,054 + Rru_A0104 0.82 +0.3 122,055 - Rru_A0104 0.82 -0.4 122,055 - Rru_A0104 0.82 +0.2 122,055 - Rru_A0104 0.82 -3.0 122,055 - Rru_A0104 0.82 +0.5 122,055 - Rru_A0104 0.82 +1.2 122,202 - Rru_A0104 0.84 -0.5 122,202 - Rru_A0104 0.84 +0.2 122,849 + -1.3 122,849 + +0.1 122,849 + -1.6 122,849 + -0.7 122,850 - -0.3 122,850 - +0.5 122,900 + +0.3 122,901 - +0.9 122,901 - -3.4 122,901 - -0.7 122,959 + -0.3 122,960 - +0.7 123,262 + Rru_A0105 0.11 -0.1 123,262 + Rru_A0105 0.11 +0.4 123,262 + Rru_A0105 0.11 -4.2 123,263 - Rru_A0105 0.11 +0.2 123,517 + Rru_A0105 0.23 -0.2 123,518 - Rru_A0105 0.23 +0.3 124,007 - Rru_A0105 0.46 +0.5 124,087 + Rru_A0105 0.50 -2.1 124,111 - Rru_A0105 0.51 -2.3 124,162 + Rru_A0105 0.53 -0.4 124,162 + Rru_A0105 0.53 -0.2 124,600 + Rru_A0105 0.74 -3.1 124,601 - Rru_A0105 0.74 -0.5 125,161 - -1.5 125,177 - -3.9 125,625 - Rru_A0106 0.47 -3.5 125,788 - Rru_A0106 0.68 -0.5 125,788 - Rru_A0106 0.68 +1.7 125,788 - Rru_A0106 0.68 +0.9
Or see this region's nucleotide sequence