Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0048 and Rru_A0049 are separated by 78 nucleotides Rru_A0049 and Rru_A0050 overlap by 4 nucleotides Rru_A0050 and Rru_A0051 are separated by 12 nucleotides Rru_A0051 and Rru_A0052 are separated by 305 nucleotides
Rru_A0048: Rru_A0048 - Lysine exporter protein (LYSE/YGGA) (NCBI), at 50,520 to 51,146
_A0048
Rru_A0049: Rru_A0049 - Biotin/lipoyl attachment (NCBI), at 51,225 to 51,629
_A0049
Rru_A0050: Rru_A0050 - hypothetical protein (NCBI), at 51,626 to 52,033
_A0050
Rru_A0051: Rru_A0051 - Carboxyl transferase (NCBI), at 52,046 to 53,599
_A0051
Rru_A0052: Rru_A0052 - Biotin carboxylase (NCBI), at 53,905 to 55,893
_A0052
Position (kb)
52
53
54 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.078 kb on - strand, within Rru_A0048 at 51.078 kb on - strand, within Rru_A0048 at 51.646 kb on + strand at 51.754 kb on + strand, within Rru_A0050 at 51.755 kb on - strand, within Rru_A0050 at 51.755 kb on - strand, within Rru_A0050 at 51.781 kb on + strand, within Rru_A0050 at 51.781 kb on + strand, within Rru_A0050 at 51.781 kb on + strand, within Rru_A0050 at 51.782 kb on - strand, within Rru_A0050 at 51.786 kb on + strand, within Rru_A0050 at 52.047 kb on + strand at 52.047 kb on + strand at 52.047 kb on + strand at 52.047 kb on + strand at 52.185 kb on + strand at 52.185 kb on + strand at 52.185 kb on + strand at 52.209 kb on + strand, within Rru_A0051 at 52.210 kb on - strand, within Rru_A0051 at 52.272 kb on + strand, within Rru_A0051 at 52.273 kb on - strand, within Rru_A0051 at 52.387 kb on - strand, within Rru_A0051 at 52.387 kb on - strand, within Rru_A0051 at 52.434 kb on + strand, within Rru_A0051 at 52.434 kb on + strand, within Rru_A0051 at 52.629 kb on + strand, within Rru_A0051 at 52.629 kb on + strand, within Rru_A0051 at 52.630 kb on - strand, within Rru_A0051 at 52.630 kb on - strand, within Rru_A0051 at 52.630 kb on - strand, within Rru_A0051 at 52.825 kb on + strand, within Rru_A0051 at 52.826 kb on - strand, within Rru_A0051 at 52.826 kb on - strand, within Rru_A0051 at 52.826 kb on - strand, within Rru_A0051 at 52.921 kb on + strand, within Rru_A0051 at 53.055 kb on + strand, within Rru_A0051 at 53.073 kb on + strand, within Rru_A0051 at 53.163 kb on + strand, within Rru_A0051 at 53.163 kb on + strand, within Rru_A0051 at 53.419 kb on + strand, within Rru_A0051 at 53.420 kb on - strand, within Rru_A0051 at 53.420 kb on - strand, within Rru_A0051 at 53.463 kb on + strand at 53.709 kb on + strand at 53.709 kb on + strand at 53.710 kb on - strand at 53.710 kb on - strand at 53.906 kb on + strand at 53.907 kb on - strand at 53.907 kb on - strand at 54.114 kb on - strand, within Rru_A0052 at 54.124 kb on - strand, within Rru_A0052 at 54.124 kb on - strand, within Rru_A0052 at 54.158 kb on + strand, within Rru_A0052 at 54.158 kb on + strand, within Rru_A0052 at 54.158 kb on + strand, within Rru_A0052 at 54.158 kb on + strand, within Rru_A0052 at 54.158 kb on + strand, within Rru_A0052 at 54.312 kb on - strand, within Rru_A0052 at 54.453 kb on + strand, within Rru_A0052 at 54.453 kb on + strand, within Rru_A0052 at 54.453 kb on + strand, within Rru_A0052 at 54.453 kb on + strand, within Rru_A0052 at 54.454 kb on - strand, within Rru_A0052 at 54.557 kb on + strand, within Rru_A0052 at 54.558 kb on - strand, within Rru_A0052
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 51,077 + Rru_A0048 0.89 -0.2 51,077 + Rru_A0048 0.89 +1.2 51,077 + Rru_A0048 0.89 +0.4 51,077 + Rru_A0048 0.89 +1.1 51,077 + Rru_A0048 0.89 +0.5 51,077 + Rru_A0048 0.89 +0.1 51,078 - Rru_A0048 0.89 -2.8 51,078 - Rru_A0048 0.89 +0.8 51,646 + +1.6 51,754 + Rru_A0050 0.31 +2.4 51,755 - Rru_A0050 0.32 -1.4 51,755 - Rru_A0050 0.32 -2.0 51,781 + Rru_A0050 0.38 -2.3 51,781 + Rru_A0050 0.38 +0.7 51,781 + Rru_A0050 0.38 -0.4 51,782 - Rru_A0050 0.38 +0.5 51,786 + Rru_A0050 0.39 +1.6 52,047 + +0.4 52,047 + +1.3 52,047 + -3.6 52,047 + -4.3 52,185 + +0.3 52,185 + +1.2 52,185 + -0.2 52,209 + Rru_A0051 0.10 +0.7 52,210 - Rru_A0051 0.11 -1.3 52,272 + Rru_A0051 0.15 +1.2 52,273 - Rru_A0051 0.15 -0.7 52,387 - Rru_A0051 0.22 +0.6 52,387 - Rru_A0051 0.22 +1.2 52,434 + Rru_A0051 0.25 -0.6 52,434 + Rru_A0051 0.25 +1.3 52,629 + Rru_A0051 0.38 +0.8 52,629 + Rru_A0051 0.38 +0.2 52,630 - Rru_A0051 0.38 +0.3 52,630 - Rru_A0051 0.38 +1.3 52,630 - Rru_A0051 0.38 +0.4 52,825 + Rru_A0051 0.50 +1.5 52,826 - Rru_A0051 0.50 +0.3 52,826 - Rru_A0051 0.50 -0.3 52,826 - Rru_A0051 0.50 +0.7 52,921 + Rru_A0051 0.56 +0.4 53,055 + Rru_A0051 0.65 +3.0 53,073 + Rru_A0051 0.66 +0.8 53,163 + Rru_A0051 0.72 +0.4 53,163 + Rru_A0051 0.72 +0.7 53,419 + Rru_A0051 0.88 +0.8 53,420 - Rru_A0051 0.88 -0.2 53,420 - Rru_A0051 0.88 -1.9 53,463 + -2.5 53,709 + -2.6 53,709 + +0.9 53,710 - +0.4 53,710 - +0.3 53,906 + -0.4 53,907 - -1.0 53,907 - -0.2 54,114 - Rru_A0052 0.11 -4.1 54,124 - Rru_A0052 0.11 -4.9 54,124 - Rru_A0052 0.11 -2.1 54,158 + Rru_A0052 0.13 -2.6 54,158 + Rru_A0052 0.13 -1.8 54,158 + Rru_A0052 0.13 -3.6 54,158 + Rru_A0052 0.13 -0.7 54,158 + Rru_A0052 0.13 -2.5 54,312 - Rru_A0052 0.20 -0.6 54,453 + Rru_A0052 0.28 -2.1 54,453 + Rru_A0052 0.28 -3.1 54,453 + Rru_A0052 0.28 -2.5 54,453 + Rru_A0052 0.28 -0.9 54,454 - Rru_A0052 0.28 -4.1 54,557 + Rru_A0052 0.33 -2.5 54,558 - Rru_A0052 0.33 -5.4
Or see this region's nucleotide sequence