Experiment: L-Phenylalanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01370 and RR42_RS01375 are separated by 286 nucleotides RR42_RS01375 and RR42_RS01380 are separated by 129 nucleotides RR42_RS01380 and RR42_RS01385 are separated by 62 nucleotides
RR42_RS01370: RR42_RS01370 - phosphomethylpyrimidine kinase, at 310,365 to 311,195
_RS01370
RR42_RS01375: RR42_RS01375 - S-adenosyl-L-homocysteine hydrolase, at 311,482 to 312,915
_RS01375
RR42_RS01380: RR42_RS01380 - membrane protein, at 313,045 to 313,398
_RS01380
RR42_RS01385: RR42_RS01385 - 5,10-methylenetetrahydrofolate reductase, at 313,461 to 314,291
_RS01385
Position (kb) 311
312
313 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 311.259 kb on + strand at 311.259 kb on + strand at 311.358 kb on + strand at 311.358 kb on + strand at 311.358 kb on + strand at 313.173 kb on + strand, within RR42_RS01380 at 313.173 kb on + strand, within RR42_RS01380 at 313.174 kb on - strand, within RR42_RS01380 at 313.174 kb on - strand, within RR42_RS01380 at 313.174 kb on - strand, within RR42_RS01380 at 313.340 kb on - strand, within RR42_RS01380 at 313.717 kb on - strand, within RR42_RS01385 at 313.875 kb on + strand, within RR42_RS01385
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (N) remove 311,259 + +0.1 311,259 + +0.8 311,358 + +0.5 311,358 + +0.0 311,358 + +0.1 313,173 + RR42_RS01380 0.36 -0.1 313,173 + RR42_RS01380 0.36 -0.2 313,174 - RR42_RS01380 0.36 -3.5 313,174 - RR42_RS01380 0.36 -3.4 313,174 - RR42_RS01380 0.36 -5.0 313,340 - RR42_RS01380 0.83 -2.5 313,717 - RR42_RS01385 0.31 -4.2 313,875 + RR42_RS01385 0.50 -2.2
Or see this region's nucleotide sequence