Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_91 and Pf1N1B4_92 are separated by 132 nucleotides Pf1N1B4_92 and Pf1N1B4_93 are separated by 232 nucleotides
Pf1N1B4_91 - monooxygenase, FAD-binding, at 95,202 to 96,731
_91
Pf1N1B4_92 - Porphobilinogen synthase (EC 4.2.1.24), at 96,864 to 97,838
_92
Pf1N1B4_93 - Phenazine biosynthesis protein PhzF like, at 98,071 to 98,913
_93
Position (kb)
96
97
98 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 95.998 kb on - strand, within Pf1N1B4_91 at 95.998 kb on - strand, within Pf1N1B4_91 at 96.151 kb on + strand, within Pf1N1B4_91 at 96.151 kb on + strand, within Pf1N1B4_91 at 96.186 kb on + strand, within Pf1N1B4_91 at 96.187 kb on - strand, within Pf1N1B4_91 at 96.187 kb on - strand, within Pf1N1B4_91 at 96.382 kb on - strand, within Pf1N1B4_91 at 96.510 kb on + strand, within Pf1N1B4_91 at 96.510 kb on + strand, within Pf1N1B4_91 at 96.511 kb on - strand, within Pf1N1B4_91 at 96.624 kb on + strand at 96.624 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.645 kb on + strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.646 kb on - strand at 96.664 kb on - strand at 96.865 kb on + strand at 96.866 kb on - strand at 96.866 kb on - strand at 96.866 kb on - strand at 96.866 kb on - strand at 96.898 kb on + strand at 96.898 kb on + strand at 96.899 kb on - strand at 97.006 kb on + strand, within Pf1N1B4_92 at 97.025 kb on - strand, within Pf1N1B4_92 at 97.178 kb on - strand, within Pf1N1B4_92 at 97.178 kb on - strand, within Pf1N1B4_92 at 97.462 kb on + strand, within Pf1N1B4_92 at 97.559 kb on + strand, within Pf1N1B4_92 at 97.631 kb on - strand, within Pf1N1B4_92 at 97.890 kb on + strand at 97.891 kb on - strand at 98.084 kb on - strand at 98.094 kb on + strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.095 kb on - strand at 98.192 kb on + strand, within Pf1N1B4_93 at 98.192 kb on + strand, within Pf1N1B4_93 at 98.192 kb on + strand, within Pf1N1B4_93 at 98.193 kb on - strand, within Pf1N1B4_93 at 98.193 kb on - strand, within Pf1N1B4_93 at 98.193 kb on - strand, within Pf1N1B4_93
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 95,998 - Pf1N1B4_91 0.52 -1.5 95,998 - Pf1N1B4_91 0.52 +2.2 96,151 + Pf1N1B4_91 0.62 -0.7 96,151 + Pf1N1B4_91 0.62 -1.5 96,186 + Pf1N1B4_91 0.64 -0.7 96,187 - Pf1N1B4_91 0.64 -0.3 96,187 - Pf1N1B4_91 0.64 -1.9 96,382 - Pf1N1B4_91 0.77 -0.7 96,510 + Pf1N1B4_91 0.85 +0.3 96,510 + Pf1N1B4_91 0.85 +0.8 96,511 - Pf1N1B4_91 0.86 +1.0 96,624 + +0.8 96,624 + -2.2 96,645 + +0.8 96,645 + +2.4 96,645 + -0.7 96,645 + -1.2 96,645 + +3.3 96,645 + +1.8 96,645 + +1.9 96,645 + -2.0 96,645 + -2.2 96,645 + -0.2 96,646 - -0.9 96,646 - +1.6 96,646 - -1.4 96,646 - +0.1 96,646 - -2.1 96,646 - +1.2 96,646 - -1.2 96,664 - +1.1 96,865 + +0.8 96,866 - -0.3 96,866 - -1.0 96,866 - -0.7 96,866 - +0.8 96,898 + +0.0 96,898 + -2.5 96,899 - -1.7 97,006 + Pf1N1B4_92 0.15 +2.7 97,025 - Pf1N1B4_92 0.17 -0.3 97,178 - Pf1N1B4_92 0.32 +0.6 97,178 - Pf1N1B4_92 0.32 -2.3 97,462 + Pf1N1B4_92 0.61 -0.2 97,559 + Pf1N1B4_92 0.71 +0.2 97,631 - Pf1N1B4_92 0.79 -0.6 97,890 + -2.2 97,891 - +1.6 98,084 - -0.7 98,094 + +1.0 98,095 - +0.1 98,095 - +0.4 98,095 - -1.5 98,095 - +0.0 98,095 - -2.0 98,095 - +1.1 98,192 + Pf1N1B4_93 0.14 -0.2 98,192 + Pf1N1B4_93 0.14 +1.4 98,192 + Pf1N1B4_93 0.14 +0.6 98,193 - Pf1N1B4_93 0.14 -1.5 98,193 - Pf1N1B4_93 0.14 +0.5 98,193 - Pf1N1B4_93 0.14 +0.0
Or see this region's nucleotide sequence