Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_716 and Pf1N1B4_717 are separated by 27 nucleotides Pf1N1B4_717 and Pf1N1B4_718 are separated by 14 nucleotides
Pf1N1B4_716 - Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites, at 816,248 to 817,675
_716
Pf1N1B4_717 - Iron-regulated protein A precursor, at 817,703 to 818,767
_717
Pf1N1B4_718 - outer membrane component of uptake system, probably for ferrous iron (from data) , at 818,782 to 819,882
_718
Position (kb)
817
818
819 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 816.740 kb on - strand, within Pf1N1B4_716 at 817.093 kb on + strand, within Pf1N1B4_716 at 817.229 kb on + strand, within Pf1N1B4_716 at 817.229 kb on + strand, within Pf1N1B4_716 at 817.229 kb on + strand, within Pf1N1B4_716 at 817.231 kb on + strand, within Pf1N1B4_716 at 817.398 kb on - strand, within Pf1N1B4_716 at 817.673 kb on + strand at 817.676 kb on + strand at 817.677 kb on - strand at 817.823 kb on + strand, within Pf1N1B4_717 at 817.824 kb on - strand, within Pf1N1B4_717 at 818.101 kb on - strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.129 kb on + strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.130 kb on - strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.183 kb on + strand, within Pf1N1B4_717 at 818.184 kb on - strand, within Pf1N1B4_717 at 818.184 kb on - strand, within Pf1N1B4_717 at 818.184 kb on - strand, within Pf1N1B4_717 at 818.580 kb on - strand, within Pf1N1B4_717 at 818.672 kb on + strand at 818.672 kb on + strand at 818.672 kb on + strand at 818.672 kb on + strand at 818.918 kb on - strand, within Pf1N1B4_718 at 819.011 kb on + strand, within Pf1N1B4_718 at 819.050 kb on - strand, within Pf1N1B4_718 at 819.115 kb on + strand, within Pf1N1B4_718 at 819.115 kb on + strand, within Pf1N1B4_718 at 819.154 kb on + strand, within Pf1N1B4_718 at 819.155 kb on - strand, within Pf1N1B4_718 at 819.205 kb on + strand, within Pf1N1B4_718 at 819.205 kb on + strand, within Pf1N1B4_718 at 819.206 kb on - strand, within Pf1N1B4_718 at 819.206 kb on - strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.207 kb on + strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.208 kb on - strand, within Pf1N1B4_718 at 819.386 kb on + strand, within Pf1N1B4_718 at 819.604 kb on + strand, within Pf1N1B4_718 at 819.604 kb on + strand, within Pf1N1B4_718 at 819.605 kb on - strand, within Pf1N1B4_718 at 819.678 kb on + strand, within Pf1N1B4_718 at 819.679 kb on - strand, within Pf1N1B4_718 at 819.679 kb on - strand, within Pf1N1B4_718 at 819.704 kb on + strand, within Pf1N1B4_718 at 819.705 kb on - strand, within Pf1N1B4_718
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 816,740 - Pf1N1B4_716 0.34 -0.4 817,093 + Pf1N1B4_716 0.59 -0.8 817,229 + Pf1N1B4_716 0.69 -2.2 817,229 + Pf1N1B4_716 0.69 +0.4 817,229 + Pf1N1B4_716 0.69 -0.1 817,231 + Pf1N1B4_716 0.69 -1.3 817,398 - Pf1N1B4_716 0.81 +1.0 817,673 + -0.0 817,676 + +0.4 817,677 - -1.3 817,823 + Pf1N1B4_717 0.11 -1.2 817,824 - Pf1N1B4_717 0.11 -1.2 818,101 - Pf1N1B4_717 0.37 -2.2 818,129 + Pf1N1B4_717 0.40 -0.3 818,129 + Pf1N1B4_717 0.40 -2.3 818,129 + -0.6 818,129 + Pf1N1B4_717 0.40 -1.3 818,129 + Pf1N1B4_717 0.40 -1.3 818,129 + +1.0 818,129 + Pf1N1B4_717 0.40 -0.6 818,129 + Pf1N1B4_717 0.40 -2.3 818,129 + Pf1N1B4_717 0.40 -0.0 818,129 + Pf1N1B4_717 0.40 -2.6 818,130 - Pf1N1B4_717 0.40 -0.0 818,130 - Pf1N1B4_717 0.40 -0.3 818,130 - Pf1N1B4_717 0.40 -1.6 818,130 - Pf1N1B4_717 0.40 -2.2 818,130 - Pf1N1B4_717 0.40 +0.2 818,130 - Pf1N1B4_717 0.40 -0.4 818,130 - Pf1N1B4_717 0.40 -1.6 818,130 - Pf1N1B4_717 0.40 -1.1 818,183 + Pf1N1B4_717 0.45 -0.7 818,183 + Pf1N1B4_717 0.45 -0.8 818,183 + Pf1N1B4_717 0.45 -2.0 818,183 + Pf1N1B4_717 0.45 -2.9 818,184 - Pf1N1B4_717 0.45 -1.8 818,184 - Pf1N1B4_717 0.45 -0.6 818,184 - Pf1N1B4_717 0.45 +1.0 818,580 - Pf1N1B4_717 0.82 -0.9 818,672 + -0.8 818,672 + -1.0 818,672 + +0.4 818,672 + -1.0 818,918 - Pf1N1B4_718 0.12 -2.0 819,011 + Pf1N1B4_718 0.21 -0.0 819,050 - Pf1N1B4_718 0.24 -2.3 819,115 + Pf1N1B4_718 0.30 -1.7 819,115 + Pf1N1B4_718 0.30 +0.5 819,154 + Pf1N1B4_718 0.34 -2.2 819,155 - Pf1N1B4_718 0.34 -0.3 819,205 + Pf1N1B4_718 0.38 -3.0 819,205 + Pf1N1B4_718 0.38 -0.7 819,206 - Pf1N1B4_718 0.39 -2.2 819,206 - Pf1N1B4_718 0.39 -2.2 819,207 + Pf1N1B4_718 0.39 -2.0 819,207 + Pf1N1B4_718 0.39 -2.2 819,207 + Pf1N1B4_718 0.39 -1.7 819,207 + Pf1N1B4_718 0.39 -0.3 819,207 + Pf1N1B4_718 0.39 +0.4 819,207 + Pf1N1B4_718 0.39 -0.8 819,207 + Pf1N1B4_718 0.39 -2.2 819,208 - Pf1N1B4_718 0.39 -2.7 819,208 - Pf1N1B4_718 0.39 -1.3 819,208 - Pf1N1B4_718 0.39 +0.2 819,208 - Pf1N1B4_718 0.39 -2.2 819,386 + Pf1N1B4_718 0.55 -0.6 819,604 + Pf1N1B4_718 0.75 -0.2 819,604 + Pf1N1B4_718 0.75 -0.6 819,605 - Pf1N1B4_718 0.75 -2.0 819,678 + Pf1N1B4_718 0.81 -1.8 819,679 - Pf1N1B4_718 0.81 -1.7 819,679 - Pf1N1B4_718 0.81 -2.7 819,704 + Pf1N1B4_718 0.84 -1.6 819,705 - Pf1N1B4_718 0.84 -0.0
Or see this region's nucleotide sequence