Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_597 and Pf1N1B4_598 are separated by 228 nucleotides Pf1N1B4_598 and Pf1N1B4_599 overlap by 11 nucleotides Pf1N1B4_599 and Pf1N1B4_600 are separated by 177 nucleotides
Pf1N1B4_597 - D-mannose isomerase (EC 5.3.1.7) (from data) , at 689,093 to 690,352
_597
Pf1N1B4_598 - Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster, at 690,581 to 692,050
_598
Pf1N1B4_599 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 692,040 to 692,771
_599
Pf1N1B4_600 - Glucokinase (EC 2.7.1.2), at 692,949 to 693,908
_600
Position (kb)
690
691
692
693 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 689.637 kb on + strand, within Pf1N1B4_597 at 689.638 kb on - strand, within Pf1N1B4_597 at 689.643 kb on + strand, within Pf1N1B4_597 at 689.643 kb on + strand at 689.644 kb on - strand, within Pf1N1B4_597 at 689.644 kb on - strand, within Pf1N1B4_597 at 689.644 kb on - strand, within Pf1N1B4_597 at 689.691 kb on + strand, within Pf1N1B4_597 at 689.692 kb on - strand, within Pf1N1B4_597 at 689.692 kb on - strand, within Pf1N1B4_597 at 689.692 kb on - strand, within Pf1N1B4_597 at 689.692 kb on - strand, within Pf1N1B4_597 at 689.829 kb on + strand, within Pf1N1B4_597 at 689.857 kb on + strand, within Pf1N1B4_597 at 690.000 kb on + strand, within Pf1N1B4_597 at 690.000 kb on + strand, within Pf1N1B4_597 at 690.163 kb on + strand, within Pf1N1B4_597 at 690.164 kb on - strand, within Pf1N1B4_597 at 690.164 kb on - strand, within Pf1N1B4_597 at 690.373 kb on + strand at 690.373 kb on + strand at 690.374 kb on - strand at 690.374 kb on - strand at 690.374 kb on - strand at 690.390 kb on + strand at 690.391 kb on - strand at 690.432 kb on + strand at 690.432 kb on + strand at 690.432 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.468 kb on + strand at 690.469 kb on - strand at 690.469 kb on - strand at 690.469 kb on - strand at 690.469 kb on - strand at 690.593 kb on - strand at 690.847 kb on - strand, within Pf1N1B4_598 at 690.967 kb on - strand, within Pf1N1B4_598 at 691.455 kb on + strand, within Pf1N1B4_598 at 691.482 kb on + strand, within Pf1N1B4_598 at 691.482 kb on + strand, within Pf1N1B4_598 at 691.482 kb on + strand, within Pf1N1B4_598 at 691.482 kb on + strand, within Pf1N1B4_598 at 691.483 kb on - strand, within Pf1N1B4_598 at 691.483 kb on - strand, within Pf1N1B4_598 at 691.483 kb on - strand, within Pf1N1B4_598 at 691.483 kb on - strand, within Pf1N1B4_598 at 691.483 kb on - strand, within Pf1N1B4_598 at 691.557 kb on + strand, within Pf1N1B4_598 at 691.557 kb on + strand, within Pf1N1B4_598 at 691.791 kb on - strand, within Pf1N1B4_598 at 692.081 kb on - strand at 692.458 kb on + strand, within Pf1N1B4_599 at 692.701 kb on + strand at 692.701 kb on + strand at 692.701 kb on + strand at 692.701 kb on + strand at 692.701 kb on + strand at 692.702 kb on - strand at 692.702 kb on - strand at 692.702 kb on - strand at 692.702 kb on - strand at 692.702 kb on - strand at 692.722 kb on - strand at 692.722 kb on - strand at 692.807 kb on + strand at 692.807 kb on + strand at 692.807 kb on + strand at 692.807 kb on + strand at 692.807 kb on + strand at 692.808 kb on - strand at 692.808 kb on - strand at 692.814 kb on + strand at 692.814 kb on + strand at 692.828 kb on - strand at 692.828 kb on - strand at 692.844 kb on + strand at 692.844 kb on + strand at 692.845 kb on - strand at 692.845 kb on - strand at 693.040 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 689,637 + Pf1N1B4_597 0.43 -0.1 689,638 - Pf1N1B4_597 0.43 -0.1 689,643 + Pf1N1B4_597 0.44 +0.7 689,643 + -0.1 689,644 - Pf1N1B4_597 0.44 -0.2 689,644 - Pf1N1B4_597 0.44 -1.0 689,644 - Pf1N1B4_597 0.44 -1.1 689,691 + Pf1N1B4_597 0.47 -2.5 689,692 - Pf1N1B4_597 0.48 +1.2 689,692 - Pf1N1B4_597 0.48 -0.4 689,692 - Pf1N1B4_597 0.48 -1.4 689,692 - Pf1N1B4_597 0.48 -1.6 689,829 + Pf1N1B4_597 0.58 -0.6 689,857 + Pf1N1B4_597 0.61 -1.1 690,000 + Pf1N1B4_597 0.72 +0.9 690,000 + Pf1N1B4_597 0.72 -0.1 690,163 + Pf1N1B4_597 0.85 -1.1 690,164 - Pf1N1B4_597 0.85 +0.5 690,164 - Pf1N1B4_597 0.85 +0.3 690,373 + -0.8 690,373 + +0.7 690,374 - -0.5 690,374 - +0.0 690,374 - -1.0 690,390 + -0.3 690,391 - +0.9 690,432 + -0.7 690,432 + +2.5 690,432 + -0.6 690,468 + -0.3 690,468 + -0.5 690,468 + +0.9 690,468 + -0.3 690,468 + -1.1 690,468 + -0.9 690,468 + -1.2 690,468 + +0.5 690,468 + +0.2 690,468 + -0.9 690,469 - -0.9 690,469 - +0.9 690,469 - -1.9 690,469 - -0.1 690,593 - -1.1 690,847 - Pf1N1B4_598 0.18 -0.1 690,967 - Pf1N1B4_598 0.26 -0.2 691,455 + Pf1N1B4_598 0.59 -0.8 691,482 + Pf1N1B4_598 0.61 +0.4 691,482 + Pf1N1B4_598 0.61 -2.1 691,482 + Pf1N1B4_598 0.61 -0.9 691,482 + Pf1N1B4_598 0.61 -2.4 691,483 - Pf1N1B4_598 0.61 +1.9 691,483 - Pf1N1B4_598 0.61 -2.1 691,483 - Pf1N1B4_598 0.61 +0.1 691,483 - Pf1N1B4_598 0.61 -1.4 691,483 - Pf1N1B4_598 0.61 -0.3 691,557 + Pf1N1B4_598 0.66 -0.9 691,557 + Pf1N1B4_598 0.66 -2.0 691,791 - Pf1N1B4_598 0.82 -0.5 692,081 - -1.4 692,458 + Pf1N1B4_599 0.57 +0.9 692,701 + -0.2 692,701 + -0.5 692,701 + +0.5 692,701 + -0.3 692,701 + -0.1 692,702 - -0.6 692,702 - +0.5 692,702 - +0.5 692,702 - +0.1 692,702 - -0.6 692,722 - -0.4 692,722 - -0.2 692,807 + +0.5 692,807 + +1.3 692,807 + +0.5 692,807 + +0.0 692,807 + -1.4 692,808 - +0.5 692,808 - +0.5 692,814 + -1.3 692,814 + -0.1 692,828 - -0.1 692,828 - +0.5 692,844 + +1.3 692,844 + -0.6 692,845 - +0.1 692,845 - +1.4 693,040 + -0.1
Or see this region's nucleotide sequence