Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5815

Experiment: Rifampicin 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5813 and Pf1N1B4_5814 are separated by 60 nucleotidesPf1N1B4_5814 and Pf1N1B4_5815 are separated by 15 nucleotidesPf1N1B4_5815 and Pf1N1B4_5816 are separated by 150 nucleotidesPf1N1B4_5816 and Pf1N1B4_5817 are separated by 227 nucleotides Pf1N1B4_5813 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 245,881 to 246,756 _5813 Pf1N1B4_5814 - putative membrane protein, at 246,817 to 247,203 _5814 Pf1N1B4_5815 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 247,219 to 248,796 _5815 Pf1N1B4_5816 - FIG00957304: hypothetical protein, at 248,947 to 249,474 _5816 Pf1N1B4_5817 - Decarboxylase family protein, at 249,702 to 251,075 _5817 Position (kb) 247 248 249Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 246.458 kb on + strand, within Pf1N1B4_5813at 246.582 kb on - strand, within Pf1N1B4_5813at 246.755 kb on - strandat 246.797 kb on + strandat 247.057 kb on + strand, within Pf1N1B4_5814at 247.057 kb on + strand, within Pf1N1B4_5814at 247.058 kb on - strand, within Pf1N1B4_5814at 247.058 kb on - strand, within Pf1N1B4_5814at 247.147 kb on + strand, within Pf1N1B4_5814at 247.148 kb on - strand, within Pf1N1B4_5814at 247.148 kb on - strand, within Pf1N1B4_5814at 247.265 kb on + strandat 247.265 kb on + strandat 247.266 kb on - strandat 247.734 kb on + strand, within Pf1N1B4_5815at 247.785 kb on + strand, within Pf1N1B4_5815at 247.788 kb on + strand, within Pf1N1B4_5815at 247.789 kb on - strand, within Pf1N1B4_5815at 248.186 kb on + strand, within Pf1N1B4_5815at 248.186 kb on + strandat 248.187 kb on - strand, within Pf1N1B4_5815at 248.327 kb on + strand, within Pf1N1B4_5815at 248.327 kb on + strandat 248.336 kb on + strand, within Pf1N1B4_5815at 248.815 kb on + strandat 248.883 kb on - strandat 248.900 kb on - strandat 248.949 kb on - strandat 249.376 kb on + strandat 249.377 kb on - strand, within Pf1N1B4_5816at 249.441 kb on - strandat 249.502 kb on - strandat 249.555 kb on + strandat 249.555 kb on + strandat 249.555 kb on + strandat 249.555 kb on + strandat 249.555 kb on + strandat 249.555 kb on + strandat 249.556 kb on - strandat 249.556 kb on - strandat 249.560 kb on - strandat 249.560 kb on - strandat 249.560 kb on - strandat 249.611 kb on - strandat 249.642 kb on + strandat 249.649 kb on + strandat 249.718 kb on + strandat 249.718 kb on + strandat 249.718 kb on + strandat 249.719 kb on - strandat 249.719 kb on - strandat 249.719 kb on - strandat 249.719 kb on - strandat 249.719 kb on - strandat 249.741 kb on - strandat 249.776 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml
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246,458 + Pf1N1B4_5813 0.66 -0.1
246,582 - Pf1N1B4_5813 0.80 +3.1
246,755 - -1.3
246,797 + -0.1
247,057 + Pf1N1B4_5814 0.62 -0.3
247,057 + Pf1N1B4_5814 0.62 +0.2
247,058 - Pf1N1B4_5814 0.62 -1.8
247,058 - Pf1N1B4_5814 0.62 +1.5
247,147 + Pf1N1B4_5814 0.85 -2.3
247,148 - Pf1N1B4_5814 0.86 -1.8
247,148 - Pf1N1B4_5814 0.86 +0.3
247,265 + -1.0
247,265 + -0.3
247,266 - -0.6
247,734 + Pf1N1B4_5815 0.33 -0.8
247,785 + Pf1N1B4_5815 0.36 +1.7
247,788 + Pf1N1B4_5815 0.36 -1.6
247,789 - Pf1N1B4_5815 0.36 -1.3
248,186 + Pf1N1B4_5815 0.61 -1.5
248,186 + +2.5
248,187 - Pf1N1B4_5815 0.61 +0.0
248,327 + Pf1N1B4_5815 0.70 -0.5
248,327 + +1.3
248,336 + Pf1N1B4_5815 0.71 -3.2
248,815 + -0.3
248,883 - +0.2
248,900 - -2.6
248,949 - -1.1
249,376 + +2.1
249,377 - Pf1N1B4_5816 0.81 +0.2
249,441 - -0.1
249,502 - +1.0
249,555 + +0.2
249,555 + +0.7
249,555 + -0.5
249,555 + +0.7
249,555 + +1.2
249,555 + -0.1
249,556 - +0.2
249,556 - -1.8
249,560 - +2.0
249,560 - -0.0
249,560 - -1.7
249,611 - -0.3
249,642 + -1.8
249,649 + +2.1
249,718 + -1.6
249,718 + -0.8
249,718 + -1.2
249,719 - -0.8
249,719 - -0.3
249,719 - +1.6
249,719 - +1.1
249,719 - +0.7
249,741 - +2.6
249,776 - +1.2

Or see this region's nucleotide sequence