Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5782 and Pf1N1B4_5783 are separated by 76 nucleotides Pf1N1B4_5783 and Pf1N1B4_5784 are separated by 22 nucleotides Pf1N1B4_5784 and Pf1N1B4_5785 overlap by 28 nucleotides
Pf1N1B4_5782 - Nitrous oxide reductase maturation protein NosR, at 219,839 to 222,052
_5782
Pf1N1B4_5783 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 222,129 to 223,517
_5783
Pf1N1B4_5784 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 223,540 to 224,085
_5784
Pf1N1B4_5785 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 224,058 to 224,423
_5785
Position (kb) 222
223
224 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 221.244 kb on + strand, within Pf1N1B4_5782 at 221.245 kb on - strand at 221.684 kb on + strand, within Pf1N1B4_5782 at 221.684 kb on + strand at 221.684 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.688 kb on + strand, within Pf1N1B4_5782 at 221.689 kb on - strand, within Pf1N1B4_5782 at 222.056 kb on - strand at 222.493 kb on + strand, within Pf1N1B4_5783 at 222.494 kb on - strand, within Pf1N1B4_5783 at 222.494 kb on - strand, within Pf1N1B4_5783 at 222.649 kb on + strand, within Pf1N1B4_5783 at 222.649 kb on + strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.650 kb on - strand, within Pf1N1B4_5783 at 222.655 kb on + strand, within Pf1N1B4_5783 at 222.776 kb on - strand, within Pf1N1B4_5783 at 222.781 kb on + strand at 223.156 kb on + strand at 223.307 kb on - strand, within Pf1N1B4_5783 at 223.307 kb on - strand, within Pf1N1B4_5783 at 223.309 kb on + strand at 223.309 kb on + strand at 223.342 kb on + strand, within Pf1N1B4_5783 at 223.342 kb on + strand at 223.342 kb on + strand, within Pf1N1B4_5783 at 223.451 kb on + strand at 223.451 kb on + strand at 223.452 kb on - strand at 223.452 kb on - strand at 223.453 kb on + strand at 223.475 kb on - strand at 223.883 kb on + strand, within Pf1N1B4_5784 at 223.883 kb on + strand, within Pf1N1B4_5784 at 223.883 kb on + strand, within Pf1N1B4_5784 at 224.039 kb on + strand at 224.039 kb on + strand at 224.039 kb on + strand at 224.039 kb on + strand at 224.040 kb on - strand at 224.165 kb on - strand, within Pf1N1B4_5785 at 224.165 kb on - strand at 224.335 kb on + strand, within Pf1N1B4_5785 at 224.464 kb on + strand at 224.464 kb on + strand at 224.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 221,244 + Pf1N1B4_5782 0.63 +1.7 221,245 - -0.3 221,684 + Pf1N1B4_5782 0.83 -0.3 221,684 + -0.3 221,684 + Pf1N1B4_5782 0.83 +2.3 221,688 + Pf1N1B4_5782 0.84 +0.7 221,688 + Pf1N1B4_5782 0.84 -0.9 221,688 + Pf1N1B4_5782 0.84 -0.3 221,688 + Pf1N1B4_5782 0.84 +1.5 221,688 + Pf1N1B4_5782 0.84 +1.2 221,688 + Pf1N1B4_5782 0.84 +0.1 221,688 + Pf1N1B4_5782 0.84 +2.2 221,688 + Pf1N1B4_5782 0.84 +1.1 221,689 - Pf1N1B4_5782 0.84 +1.2 222,056 - +0.5 222,493 + Pf1N1B4_5783 0.26 +0.3 222,494 - Pf1N1B4_5783 0.26 -0.1 222,494 - Pf1N1B4_5783 0.26 -0.4 222,649 + Pf1N1B4_5783 0.37 +0.7 222,649 + Pf1N1B4_5783 0.37 +0.7 222,650 - Pf1N1B4_5783 0.38 +1.1 222,650 - Pf1N1B4_5783 0.38 -0.5 222,650 - Pf1N1B4_5783 0.38 -0.9 222,655 + Pf1N1B4_5783 0.38 -0.5 222,776 - Pf1N1B4_5783 0.47 -0.4 222,781 + -0.3 223,156 + -1.9 223,307 - Pf1N1B4_5783 0.85 +0.1 223,307 - Pf1N1B4_5783 0.85 -0.6 223,309 + +1.9 223,309 + +0.9 223,342 + Pf1N1B4_5783 0.87 +0.1 223,342 + -0.0 223,342 + Pf1N1B4_5783 0.87 -0.0 223,451 + -2.5 223,451 + -2.3 223,452 - +3.3 223,452 - +1.9 223,453 + +0.4 223,475 - -0.9 223,883 + Pf1N1B4_5784 0.63 -1.9 223,883 + Pf1N1B4_5784 0.63 +1.1 223,883 + Pf1N1B4_5784 0.63 +0.2 224,039 + +0.3 224,039 + +0.7 224,039 + -0.0 224,039 + +2.7 224,040 - +0.4 224,165 - Pf1N1B4_5785 0.29 +1.7 224,165 - -0.9 224,335 + Pf1N1B4_5785 0.76 -1.3 224,464 + -0.5 224,464 + +0.9 224,465 - +0.7
Or see this region's nucleotide sequence