Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_577 and Pf1N1B4_578 are separated by 45 nucleotides Pf1N1B4_578 and Pf1N1B4_579 are separated by 83 nucleotides Pf1N1B4_579 and Pf1N1B4_580 are separated by 11 nucleotides
Pf1N1B4_577 - Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative, at 669,959 to 670,903
_577
Pf1N1B4_578 - enoyl-CoA hydratase, R-specific, at 670,949 to 671,419
_578
Pf1N1B4_579 - Membrane fusion component of tripartite multidrug resistance system, at 671,503 to 672,363
_579
Pf1N1B4_580 - FIG00953813: hypothetical protein, at 672,375 to 672,581
_580
Position (kb)
670
671
672 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 670.125 kb on - strand, within Pf1N1B4_577 at 670.133 kb on + strand, within Pf1N1B4_577 at 670.133 kb on + strand, within Pf1N1B4_577 at 670.133 kb on + strand, within Pf1N1B4_577 at 670.146 kb on + strand, within Pf1N1B4_577 at 670.146 kb on + strand, within Pf1N1B4_577 at 670.147 kb on - strand at 670.147 kb on - strand, within Pf1N1B4_577 at 670.147 kb on - strand, within Pf1N1B4_577 at 670.282 kb on - strand, within Pf1N1B4_577 at 670.282 kb on - strand, within Pf1N1B4_577 at 670.307 kb on + strand, within Pf1N1B4_577 at 670.307 kb on + strand, within Pf1N1B4_577 at 670.308 kb on - strand, within Pf1N1B4_577 at 670.308 kb on - strand, within Pf1N1B4_577 at 670.322 kb on + strand, within Pf1N1B4_577 at 670.322 kb on + strand, within Pf1N1B4_577 at 670.322 kb on + strand, within Pf1N1B4_577 at 670.323 kb on - strand at 670.323 kb on - strand, within Pf1N1B4_577 at 670.559 kb on + strand, within Pf1N1B4_577 at 670.559 kb on + strand, within Pf1N1B4_577 at 670.559 kb on + strand, within Pf1N1B4_577 at 670.560 kb on - strand, within Pf1N1B4_577 at 670.783 kb on + strand, within Pf1N1B4_577 at 670.783 kb on + strand, within Pf1N1B4_577 at 670.783 kb on + strand, within Pf1N1B4_577 at 671.006 kb on + strand, within Pf1N1B4_578 at 671.119 kb on + strand, within Pf1N1B4_578 at 671.195 kb on + strand, within Pf1N1B4_578 at 671.196 kb on - strand, within Pf1N1B4_578 at 671.197 kb on + strand, within Pf1N1B4_578 at 671.292 kb on + strand at 671.292 kb on + strand, within Pf1N1B4_578 at 671.422 kb on + strand at 671.422 kb on + strand at 671.423 kb on - strand at 671.458 kb on + strand at 671.458 kb on + strand at 671.665 kb on - strand, within Pf1N1B4_579 at 671.665 kb on - strand, within Pf1N1B4_579 at 671.789 kb on + strand, within Pf1N1B4_579 at 671.801 kb on + strand, within Pf1N1B4_579 at 671.802 kb on - strand, within Pf1N1B4_579 at 671.802 kb on - strand, within Pf1N1B4_579 at 671.802 kb on - strand, within Pf1N1B4_579 at 671.802 kb on - strand, within Pf1N1B4_579 at 671.802 kb on - strand at 671.852 kb on + strand, within Pf1N1B4_579 at 671.852 kb on + strand, within Pf1N1B4_579 at 671.852 kb on + strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.853 kb on - strand, within Pf1N1B4_579 at 671.856 kb on + strand, within Pf1N1B4_579 at 671.856 kb on + strand, within Pf1N1B4_579 at 671.857 kb on - strand, within Pf1N1B4_579 at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.281 kb on + strand at 672.282 kb on - strand at 672.282 kb on - strand at 672.282 kb on - strand at 672.282 kb on - strand at 672.284 kb on + strand at 672.350 kb on - strand at 672.357 kb on + strand at 672.357 kb on + strand at 672.357 kb on + strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.358 kb on - strand at 672.364 kb on - strand at 672.364 kb on - strand at 672.377 kb on - strand at 672.378 kb on + strand at 672.412 kb on - strand, within Pf1N1B4_580 at 672.412 kb on - strand, within Pf1N1B4_580 at 672.412 kb on - strand, within Pf1N1B4_580 at 672.412 kb on - strand, within Pf1N1B4_580
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 670,125 - Pf1N1B4_577 0.18 +0.3 670,133 + Pf1N1B4_577 0.18 +0.3 670,133 + Pf1N1B4_577 0.18 -2.4 670,133 + Pf1N1B4_577 0.18 -2.2 670,146 + Pf1N1B4_577 0.20 +0.7 670,146 + Pf1N1B4_577 0.20 -1.7 670,147 - +2.5 670,147 - Pf1N1B4_577 0.20 +0.2 670,147 - Pf1N1B4_577 0.20 -0.3 670,282 - Pf1N1B4_577 0.34 -0.7 670,282 - Pf1N1B4_577 0.34 -0.7 670,307 + Pf1N1B4_577 0.37 -0.1 670,307 + Pf1N1B4_577 0.37 +0.1 670,308 - Pf1N1B4_577 0.37 -1.7 670,308 - Pf1N1B4_577 0.37 -1.1 670,322 + Pf1N1B4_577 0.38 +1.3 670,322 + Pf1N1B4_577 0.38 +0.9 670,322 + Pf1N1B4_577 0.38 -1.8 670,323 - +2.9 670,323 - Pf1N1B4_577 0.39 +0.3 670,559 + Pf1N1B4_577 0.63 +1.7 670,559 + Pf1N1B4_577 0.63 -0.8 670,559 + Pf1N1B4_577 0.63 -0.4 670,560 - Pf1N1B4_577 0.64 -2.1 670,783 + Pf1N1B4_577 0.87 -0.7 670,783 + Pf1N1B4_577 0.87 -2.4 670,783 + Pf1N1B4_577 0.87 -1.2 671,006 + Pf1N1B4_578 0.12 -2.0 671,119 + Pf1N1B4_578 0.36 -0.7 671,195 + Pf1N1B4_578 0.52 -0.9 671,196 - Pf1N1B4_578 0.52 -1.7 671,197 + Pf1N1B4_578 0.53 -0.1 671,292 + -0.1 671,292 + Pf1N1B4_578 0.73 +0.3 671,422 + -0.1 671,422 + -1.4 671,423 - -1.1 671,458 + +1.5 671,458 + -1.0 671,665 - Pf1N1B4_579 0.19 -0.2 671,665 - Pf1N1B4_579 0.19 -0.1 671,789 + Pf1N1B4_579 0.33 +0.9 671,801 + Pf1N1B4_579 0.35 +0.3 671,802 - Pf1N1B4_579 0.35 -0.7 671,802 - Pf1N1B4_579 0.35 +1.2 671,802 - Pf1N1B4_579 0.35 +2.5 671,802 - Pf1N1B4_579 0.35 +1.7 671,802 - -1.1 671,852 + Pf1N1B4_579 0.41 -1.9 671,852 + Pf1N1B4_579 0.41 -0.4 671,852 + Pf1N1B4_579 0.41 +2.2 671,853 - Pf1N1B4_579 0.41 -1.7 671,853 - Pf1N1B4_579 0.41 +1.9 671,853 - Pf1N1B4_579 0.41 +1.3 671,853 - Pf1N1B4_579 0.41 -0.9 671,853 - Pf1N1B4_579 0.41 -0.4 671,856 + Pf1N1B4_579 0.41 -0.7 671,856 + Pf1N1B4_579 0.41 -1.1 671,857 - Pf1N1B4_579 0.41 -1.7 672,281 + -0.6 672,281 + -1.1 672,281 + -2.1 672,281 + -1.4 672,281 + -0.1 672,281 + -0.1 672,281 + -0.6 672,281 + -0.1 672,281 + -0.1 672,281 + -0.5 672,282 - +1.1 672,282 - -0.1 672,282 - +0.6 672,282 - +0.6 672,284 + +1.4 672,350 - -0.1 672,357 + -2.4 672,357 + +1.2 672,357 + -0.5 672,358 - -1.9 672,358 - -0.1 672,358 - -0.3 672,358 - -0.4 672,358 - -3.8 672,358 - -1.4 672,358 - +0.0 672,358 - -1.6 672,358 - +0.6 672,358 - -0.8 672,364 - -0.1 672,364 - -1.6 672,377 - -0.1 672,378 + +0.5 672,412 - Pf1N1B4_580 0.18 -0.1 672,412 - Pf1N1B4_580 0.18 -0.5 672,412 - Pf1N1B4_580 0.18 +0.9 672,412 - Pf1N1B4_580 0.18 -0.2
Or see this region's nucleotide sequence