Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5519 and Pf1N1B4_5520 are separated by 485 nucleotides
Pf1N1B4_5519 - hypothetical protein, at 272,494 to 273,657
_5519
Pf1N1B4_5520 - General secretion pathway protein E, at 274,143 to 275,993
_5520
Position (kb) 274
275
276 Strain fitness (log2 ratio) -2
-1
0
1
2 at 273.225 kb on + strand, within Pf1N1B4_5519 at 273.245 kb on + strand, within Pf1N1B4_5519 at 273.406 kb on - strand, within Pf1N1B4_5519 at 273.679 kb on - strand at 273.791 kb on - strand at 273.861 kb on - strand at 274.735 kb on - strand, within Pf1N1B4_5520 at 274.830 kb on + strand, within Pf1N1B4_5520 at 274.831 kb on - strand, within Pf1N1B4_5520 at 274.831 kb on - strand, within Pf1N1B4_5520 at 274.897 kb on - strand, within Pf1N1B4_5520 at 274.900 kb on + strand, within Pf1N1B4_5520 at 274.900 kb on + strand, within Pf1N1B4_5520 at 274.904 kb on + strand, within Pf1N1B4_5520 at 274.904 kb on + strand at 275.023 kb on + strand, within Pf1N1B4_5520 at 275.024 kb on - strand, within Pf1N1B4_5520 at 275.108 kb on - strand, within Pf1N1B4_5520 at 275.108 kb on - strand at 275.179 kb on - strand, within Pf1N1B4_5520 at 275.220 kb on + strand, within Pf1N1B4_5520 at 275.373 kb on + strand, within Pf1N1B4_5520 at 275.424 kb on + strand, within Pf1N1B4_5520 at 275.485 kb on - strand, within Pf1N1B4_5520 at 275.670 kb on + strand, within Pf1N1B4_5520 at 275.673 kb on - strand, within Pf1N1B4_5520 at 275.719 kb on + strand at 275.735 kb on + strand, within Pf1N1B4_5520 at 275.852 kb on + strand at 275.899 kb on - strand at 275.899 kb on - strand at 275.899 kb on - strand at 275.997 kb on + strand at 276.104 kb on - strand at 276.112 kb on - strand at 276.112 kb on - strand at 276.475 kb on + strand at 276.480 kb on - strand at 276.591 kb on - strand at 276.717 kb on + strand at 276.832 kb on + strand at 276.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 273,225 + Pf1N1B4_5519 0.63 +0.5 273,245 + Pf1N1B4_5519 0.65 -1.2 273,406 - Pf1N1B4_5519 0.78 -1.1 273,679 - -1.3 273,791 - -0.5 273,861 - +0.4 274,735 - Pf1N1B4_5520 0.32 -0.4 274,830 + Pf1N1B4_5520 0.37 -0.7 274,831 - Pf1N1B4_5520 0.37 -2.0 274,831 - Pf1N1B4_5520 0.37 -1.2 274,897 - Pf1N1B4_5520 0.41 -2.4 274,900 + Pf1N1B4_5520 0.41 -0.3 274,900 + Pf1N1B4_5520 0.41 -1.2 274,904 + Pf1N1B4_5520 0.41 +2.1 274,904 + +0.1 275,023 + Pf1N1B4_5520 0.48 -1.4 275,024 - Pf1N1B4_5520 0.48 +0.7 275,108 - Pf1N1B4_5520 0.52 -0.4 275,108 - -0.4 275,179 - Pf1N1B4_5520 0.56 -1.1 275,220 + Pf1N1B4_5520 0.58 -2.0 275,373 + Pf1N1B4_5520 0.66 +0.3 275,424 + Pf1N1B4_5520 0.69 -1.4 275,485 - Pf1N1B4_5520 0.73 -2.3 275,670 + Pf1N1B4_5520 0.82 -0.3 275,673 - Pf1N1B4_5520 0.83 -0.4 275,719 + -0.9 275,735 + Pf1N1B4_5520 0.86 -2.2 275,852 + -1.4 275,899 - +0.8 275,899 - +1.1 275,899 - +2.1 275,997 + +0.1 276,104 - -1.6 276,112 - -1.4 276,112 - -1.4 276,475 + -0.8 276,480 - +1.1 276,591 - -1.4 276,717 + +0.6 276,832 + -0.4 276,833 - -0.4
Or see this region's nucleotide sequence