Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4920 and Pf1N1B4_4921 are separated by 466 nucleotides Pf1N1B4_4921 and Pf1N1B4_4922 are separated by 43 nucleotides
Pf1N1B4_4920 - Aldehyde dehydrogenase (EC 1.2.1.3), at 5,451,574 to 5,453,046
_4920
Pf1N1B4_4921 - Haloacid dehalogenase, type II, at 5,453,513 to 5,454,181
_4921
Pf1N1B4_4922 - Opine oxidase subunit B, at 5,454,225 to 5,455,499
_4922
Position (kb) 5453
5454
5455 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5452.519 kb on + strand, within Pf1N1B4_4920 at 5452.687 kb on + strand, within Pf1N1B4_4920 at 5452.688 kb on - strand, within Pf1N1B4_4920 at 5452.934 kb on - strand at 5453.288 kb on - strand at 5453.419 kb on + strand at 5453.420 kb on - strand at 5453.772 kb on - strand, within Pf1N1B4_4921 at 5453.774 kb on + strand, within Pf1N1B4_4921 at 5453.775 kb on - strand, within Pf1N1B4_4921 at 5454.041 kb on + strand, within Pf1N1B4_4921 at 5454.125 kb on + strand at 5454.125 kb on + strand at 5454.125 kb on + strand at 5454.125 kb on + strand at 5454.125 kb on + strand at 5454.125 kb on + strand at 5454.125 kb on + strand at 5454.126 kb on - strand at 5454.126 kb on - strand at 5454.126 kb on - strand at 5454.126 kb on - strand at 5454.220 kb on + strand at 5454.459 kb on + strand, within Pf1N1B4_4922 at 5454.460 kb on - strand at 5454.504 kb on + strand, within Pf1N1B4_4922 at 5454.826 kb on - strand, within Pf1N1B4_4922 at 5454.913 kb on + strand, within Pf1N1B4_4922 at 5454.913 kb on + strand, within Pf1N1B4_4922 at 5454.914 kb on - strand, within Pf1N1B4_4922 at 5454.914 kb on - strand, within Pf1N1B4_4922 at 5454.914 kb on - strand, within Pf1N1B4_4922 at 5454.914 kb on - strand, within Pf1N1B4_4922 at 5455.053 kb on + strand, within Pf1N1B4_4922 at 5455.053 kb on + strand, within Pf1N1B4_4922 at 5455.053 kb on + strand, within Pf1N1B4_4922 at 5455.054 kb on - strand, within Pf1N1B4_4922 at 5455.054 kb on - strand, within Pf1N1B4_4922 at 5455.054 kb on - strand, within Pf1N1B4_4922 at 5455.054 kb on - strand, within Pf1N1B4_4922 at 5455.054 kb on - strand at 5455.054 kb on - strand at 5455.054 kb on - strand, within Pf1N1B4_4922 at 5455.080 kb on + strand, within Pf1N1B4_4922 at 5455.080 kb on + strand, within Pf1N1B4_4922 at 5455.080 kb on + strand, within Pf1N1B4_4922 at 5455.080 kb on + strand, within Pf1N1B4_4922 at 5455.080 kb on + strand, within Pf1N1B4_4922 at 5455.081 kb on - strand, within Pf1N1B4_4922
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 5,452,519 + Pf1N1B4_4920 0.64 +1.1 5,452,687 + Pf1N1B4_4920 0.76 +1.6 5,452,688 - Pf1N1B4_4920 0.76 +0.6 5,452,934 - -1.3 5,453,288 - -1.0 5,453,419 + +1.8 5,453,420 - +0.5 5,453,772 - Pf1N1B4_4921 0.39 -0.5 5,453,774 + Pf1N1B4_4921 0.39 -2.2 5,453,775 - Pf1N1B4_4921 0.39 -1.0 5,454,041 + Pf1N1B4_4921 0.79 -0.5 5,454,125 + -0.9 5,454,125 + -0.5 5,454,125 + -0.2 5,454,125 + +0.1 5,454,125 + -1.2 5,454,125 + +1.1 5,454,125 + +0.4 5,454,126 - +0.2 5,454,126 - -0.5 5,454,126 - +0.4 5,454,126 - +0.5 5,454,220 + -0.2 5,454,459 + Pf1N1B4_4922 0.18 +1.5 5,454,460 - -1.5 5,454,504 + Pf1N1B4_4922 0.22 -0.9 5,454,826 - Pf1N1B4_4922 0.47 -2.3 5,454,913 + Pf1N1B4_4922 0.54 +2.4 5,454,913 + Pf1N1B4_4922 0.54 +0.5 5,454,914 - Pf1N1B4_4922 0.54 +0.3 5,454,914 - Pf1N1B4_4922 0.54 -0.0 5,454,914 - Pf1N1B4_4922 0.54 -2.0 5,454,914 - Pf1N1B4_4922 0.54 +0.2 5,455,053 + Pf1N1B4_4922 0.65 -1.0 5,455,053 + Pf1N1B4_4922 0.65 -1.6 5,455,053 + Pf1N1B4_4922 0.65 +1.7 5,455,054 - Pf1N1B4_4922 0.65 +0.2 5,455,054 - Pf1N1B4_4922 0.65 -1.5 5,455,054 - Pf1N1B4_4922 0.65 +2.1 5,455,054 - Pf1N1B4_4922 0.65 -0.6 5,455,054 - -2.0 5,455,054 - -0.5 5,455,054 - Pf1N1B4_4922 0.65 -0.2 5,455,080 + Pf1N1B4_4922 0.67 -0.1 5,455,080 + Pf1N1B4_4922 0.67 -1.6 5,455,080 + Pf1N1B4_4922 0.67 -0.5 5,455,080 + Pf1N1B4_4922 0.67 -2.6 5,455,080 + Pf1N1B4_4922 0.67 -0.5 5,455,081 - Pf1N1B4_4922 0.67 -2.2
Or see this region's nucleotide sequence