Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_488 and Pf1N1B4_489 are separated by 91 nucleotides Pf1N1B4_489 and Pf1N1B4_490 are separated by 466 nucleotides
Pf1N1B4_488 - Cyn operon transcriptional activator, at 580,025 to 580,972
_488
Pf1N1B4_489 - hypothetical protein possibly connected to lactam utilization and allophanate hydrolase, at 581,064 to 581,867
_489
Pf1N1B4_490 - hypothetical protein, at 582,334 to 582,507
_490
Position (kb) 581
582 Strain fitness (log2 ratio) -2
-1
0
1
2 at 580.118 kb on - strand at 580.224 kb on - strand, within Pf1N1B4_488 at 580.509 kb on - strand, within Pf1N1B4_488 at 580.544 kb on + strand, within Pf1N1B4_488 at 580.545 kb on - strand, within Pf1N1B4_488 at 580.545 kb on - strand, within Pf1N1B4_488 at 580.545 kb on - strand, within Pf1N1B4_488 at 580.545 kb on - strand, within Pf1N1B4_488 at 580.545 kb on - strand, within Pf1N1B4_488 at 580.556 kb on + strand, within Pf1N1B4_488 at 580.629 kb on + strand, within Pf1N1B4_488 at 580.629 kb on + strand, within Pf1N1B4_488 at 580.629 kb on + strand, within Pf1N1B4_488 at 580.630 kb on - strand, within Pf1N1B4_488 at 580.630 kb on - strand, within Pf1N1B4_488 at 580.630 kb on - strand, within Pf1N1B4_488 at 580.970 kb on + strand at 580.971 kb on - strand at 580.971 kb on - strand at 580.978 kb on - strand at 580.978 kb on - strand at 580.978 kb on - strand at 580.978 kb on - strand at 581.145 kb on + strand, within Pf1N1B4_489 at 581.146 kb on - strand, within Pf1N1B4_489 at 581.355 kb on + strand, within Pf1N1B4_489 at 581.518 kb on - strand, within Pf1N1B4_489 at 581.518 kb on - strand, within Pf1N1B4_489 at 581.518 kb on - strand, within Pf1N1B4_489 at 581.520 kb on - strand, within Pf1N1B4_489 at 581.530 kb on + strand, within Pf1N1B4_489 at 581.530 kb on + strand at 581.530 kb on + strand, within Pf1N1B4_489 at 581.530 kb on + strand, within Pf1N1B4_489 at 581.531 kb on - strand at 581.531 kb on - strand, within Pf1N1B4_489 at 581.531 kb on - strand, within Pf1N1B4_489 at 581.531 kb on - strand, within Pf1N1B4_489 at 581.531 kb on - strand at 581.531 kb on - strand, within Pf1N1B4_489 at 581.785 kb on + strand, within Pf1N1B4_489 at 581.786 kb on - strand, within Pf1N1B4_489 at 581.894 kb on - strand at 581.966 kb on + strand at 581.966 kb on + strand at 581.980 kb on + strand at 581.981 kb on - strand at 581.981 kb on - strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.984 kb on + strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 581.985 kb on - strand at 582.072 kb on + strand at 582.073 kb on - strand at 582.120 kb on - strand at 582.120 kb on - strand at 582.158 kb on - strand at 582.158 kb on - strand at 582.159 kb on + strand at 582.159 kb on + strand at 582.160 kb on - strand at 582.160 kb on - strand at 582.160 kb on - strand at 582.160 kb on - strand at 582.271 kb on + strand at 582.271 kb on + strand at 582.272 kb on - strand at 582.319 kb on - strand at 582.319 kb on - strand at 582.440 kb on + strand, within Pf1N1B4_490 at 582.440 kb on + strand, within Pf1N1B4_490 at 582.440 kb on + strand, within Pf1N1B4_490 at 582.441 kb on - strand, within Pf1N1B4_490 at 582.441 kb on - strand, within Pf1N1B4_490 at 582.466 kb on + strand, within Pf1N1B4_490 at 582.466 kb on + strand, within Pf1N1B4_490 at 582.480 kb on + strand, within Pf1N1B4_490 at 582.480 kb on + strand, within Pf1N1B4_490 at 582.505 kb on + strand at 582.505 kb on + strand at 582.505 kb on + strand at 582.506 kb on - strand at 582.506 kb on - strand at 582.506 kb on - strand at 582.511 kb on + strand at 582.512 kb on - strand at 582.512 kb on - strand at 582.525 kb on + strand at 582.525 kb on + strand at 582.537 kb on + strand at 582.537 kb on + strand at 582.537 kb on + strand at 582.537 kb on + strand at 582.538 kb on - strand at 582.538 kb on - strand at 582.538 kb on - strand at 582.564 kb on + strand at 582.565 kb on - strand at 582.588 kb on + strand at 582.588 kb on + strand at 582.640 kb on + strand at 582.640 kb on + strand at 582.641 kb on - strand at 582.641 kb on - strand at 582.641 kb on - strand at 582.691 kb on - strand at 582.811 kb on + strand at 582.812 kb on - strand at 582.812 kb on - strand at 582.835 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 580,118 - -1.8 580,224 - Pf1N1B4_488 0.21 -0.3 580,509 - Pf1N1B4_488 0.51 -1.4 580,544 + Pf1N1B4_488 0.55 -0.5 580,545 - Pf1N1B4_488 0.55 -0.9 580,545 - Pf1N1B4_488 0.55 +0.9 580,545 - Pf1N1B4_488 0.55 -0.5 580,545 - Pf1N1B4_488 0.55 -0.7 580,545 - Pf1N1B4_488 0.55 -2.2 580,556 + Pf1N1B4_488 0.56 -1.1 580,629 + Pf1N1B4_488 0.64 +0.3 580,629 + Pf1N1B4_488 0.64 +0.9 580,629 + Pf1N1B4_488 0.64 -1.7 580,630 - Pf1N1B4_488 0.64 -0.5 580,630 - Pf1N1B4_488 0.64 +1.3 580,630 - Pf1N1B4_488 0.64 +0.9 580,970 + +1.5 580,971 - -0.1 580,971 - +0.1 580,978 - +1.9 580,978 - -0.2 580,978 - +0.3 580,978 - +0.2 581,145 + Pf1N1B4_489 0.10 -0.1 581,146 - Pf1N1B4_489 0.10 -1.7 581,355 + Pf1N1B4_489 0.36 -1.4 581,518 - Pf1N1B4_489 0.56 +1.9 581,518 - Pf1N1B4_489 0.56 -0.7 581,518 - Pf1N1B4_489 0.56 +0.3 581,520 - Pf1N1B4_489 0.57 +0.9 581,530 + Pf1N1B4_489 0.58 +0.2 581,530 + +0.9 581,530 + Pf1N1B4_489 0.58 +0.9 581,530 + Pf1N1B4_489 0.58 +2.2 581,531 - -0.1 581,531 - Pf1N1B4_489 0.58 +0.9 581,531 - Pf1N1B4_489 0.58 +0.2 581,531 - Pf1N1B4_489 0.58 -0.8 581,531 - +0.9 581,531 - Pf1N1B4_489 0.58 -0.1 581,785 + Pf1N1B4_489 0.90 +0.9 581,786 - Pf1N1B4_489 0.90 +0.5 581,894 - -2.2 581,966 + -0.7 581,966 + -0.0 581,980 + +1.9 581,981 - -0.5 581,981 - -0.1 581,984 + -0.1 581,984 + -1.9 581,984 + -0.1 581,984 + -0.3 581,984 + -1.4 581,985 - +1.2 581,985 - +0.3 581,985 - -1.9 581,985 - +1.2 581,985 - +0.3 581,985 - +0.9 581,985 - +0.8 582,072 + +0.2 582,073 - -0.4 582,120 - -2.1 582,120 - +0.1 582,158 - +2.1 582,158 - -0.7 582,159 + -0.2 582,159 + -2.5 582,160 - -0.6 582,160 - +0.6 582,160 - +0.9 582,160 - +2.5 582,271 + -0.1 582,271 + -0.1 582,272 - +0.6 582,319 - -0.7 582,319 - -1.1 582,440 + Pf1N1B4_490 0.61 +1.2 582,440 + Pf1N1B4_490 0.61 -0.1 582,440 + Pf1N1B4_490 0.61 -1.2 582,441 - Pf1N1B4_490 0.61 +1.2 582,441 - Pf1N1B4_490 0.61 +0.3 582,466 + Pf1N1B4_490 0.76 +0.4 582,466 + Pf1N1B4_490 0.76 +0.9 582,480 + Pf1N1B4_490 0.84 -0.7 582,480 + Pf1N1B4_490 0.84 +1.3 582,505 + -0.7 582,505 + -0.7 582,505 + -0.1 582,506 - -2.2 582,506 - -0.7 582,506 - +0.5 582,511 + -0.1 582,512 - +0.7 582,512 - +1.4 582,525 + -1.1 582,525 + +0.7 582,537 + +0.9 582,537 + +1.3 582,537 + -0.5 582,537 + -2.1 582,538 - -1.1 582,538 - -0.7 582,538 - +0.5 582,564 + -0.1 582,565 - -0.9 582,588 + -1.2 582,588 + +0.9 582,640 + +0.9 582,640 + +0.9 582,641 - -0.1 582,641 - -0.0 582,641 - -0.7 582,691 - -0.1 582,811 + +0.7 582,812 - -2.2 582,812 - -0.4 582,835 + -1.1
Or see this region's nucleotide sequence