Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4586 and Pf1N1B4_4587 are separated by 35 nucleotides Pf1N1B4_4587 and Pf1N1B4_4588 are separated by 112 nucleotides Pf1N1B4_4588 and Pf1N1B4_4589 are separated by 155 nucleotides
Pf1N1B4_4586 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 5,057,264 to 5,057,872
_4586
Pf1N1B4_4587 - probable dioxygenase, at 5,057,908 to 5,058,333
_4587
Pf1N1B4_4588 - Transcriptional regulator, at 5,058,446 to 5,059,312
_4588
Pf1N1B4_4589 - Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9), at 5,059,468 to 5,060,265
_4589
Position (kb) 5058
5059
5060 Strain fitness (log2 ratio) -1
0
1
2
3 at 5057.759 kb on + strand, within Pf1N1B4_4586 at 5057.842 kb on + strand at 5058.126 kb on + strand, within Pf1N1B4_4587 at 5058.126 kb on + strand, within Pf1N1B4_4587 at 5058.126 kb on + strand, within Pf1N1B4_4587 at 5058.127 kb on - strand, within Pf1N1B4_4587 at 5058.270 kb on - strand, within Pf1N1B4_4587 at 5058.270 kb on - strand at 5058.371 kb on + strand at 5058.445 kb on + strand at 5058.445 kb on + strand at 5058.446 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 5,057,759 + Pf1N1B4_4586 0.81 +2.2 5,057,842 + +0.8 5,058,126 + Pf1N1B4_4587 0.51 -1.4 5,058,126 + Pf1N1B4_4587 0.51 +1.2 5,058,126 + Pf1N1B4_4587 0.51 +0.6 5,058,127 - Pf1N1B4_4587 0.51 +0.1 5,058,270 - Pf1N1B4_4587 0.85 +1.1 5,058,270 - +0.8 5,058,371 + +3.0 5,058,445 + +1.2 5,058,445 + +1.0 5,058,446 - +1.2
Or see this region's nucleotide sequence