Experiment: Rifampicin 0.004 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_4480 and Pf1N1B4_4481 are separated by 185 nucleotides Pf1N1B4_4481 and Pf1N1B4_4482 are separated by 128 nucleotides Pf1N1B4_4482 and Pf1N1B4_4483 are separated by 104 nucleotides Pf1N1B4_4483 and Pf1N1B4_4484 are separated by 183 nucleotides  
        
        Pf1N1B4_4480 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 4,941,511 to 4,942,746 
         
        _4480 
         
        
        Pf1N1B4_4481 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 4,942,932 to 4,943,420 
         
        _4481 
         
        
        Pf1N1B4_4482 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 4,943,549 to 4,944,412 
         
        _4482 
         
        
        Pf1N1B4_4483 - Transcriptional regulator, ArsR family, at 4,944,517 to 4,944,852 
         
        _4483 
         
        
        Pf1N1B4_4484 - FIG002984: FAD-dependent pyridine nucleotide-disulphide oxidoreductase, at 4,945,036 to 4,946,280 
         
        _4484 
         Position (kb)  
4943 
 
4944 
 
4945 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 4942.668 kb on - strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.779 kb on - strand at 4942.779 kb on - strand at 4942.851 kb on - strand at 4942.904 kb on - strand at 4943.226 kb on + strand, within Pf1N1B4_4481 at 4943.251 kb on - strand, within Pf1N1B4_4481 at 4943.251 kb on - strand at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.550 kb on + strand at 4943.550 kb on + strand at 4943.578 kb on - strand at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.958 kb on + strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.978 kb on - strand, within Pf1N1B4_4482 at 4944.303 kb on + strand, within Pf1N1B4_4482 at 4944.351 kb on + strand at 4944.352 kb on - strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.361 kb on - strand at 4944.366 kb on + strand at 4944.367 kb on - strand at 4944.367 kb on - strand at 4944.465 kb on + strand at 4944.465 kb on + strand at 4944.466 kb on - strand at 4944.735 kb on - strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.758 kb on - strand, within Pf1N1B4_4483 at 4944.758 kb on - strand at 4944.758 kb on - strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.762 kb on - strand, within Pf1N1B4_4483 at 4944.766 kb on + strand, within Pf1N1B4_4483 at 4944.766 kb on + strand, within Pf1N1B4_4483 at 4944.767 kb on - strand, within Pf1N1B4_4483 at 4944.820 kb on + strand at 4944.821 kb on - strand at 4944.977 kb on + strand at 4945.021 kb on - strand at 4945.083 kb on - strand at 4945.085 kb on + strand at 4945.086 kb on - strand at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.184 kb on - strand, within Pf1N1B4_4484 at 4945.220 kb on - strand, within Pf1N1B4_4484 at 4945.220 kb on - strand, within Pf1N1B4_4484  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Rifampicin 0.004 mg/ml             remove   4,942,668   -        -1.4   4,942,753   +        +1.6   4,942,753   +        +1.4   4,942,753   +        -0.4   4,942,779   -        +0.6   4,942,779   -        -1.4   4,942,851   -        +0.2   4,942,904   -        -1.4   4,943,226   +    Pf1N1B4_4481   0.60  +0.6   4,943,251   -    Pf1N1B4_4481   0.65  +0.6   4,943,251   -        +1.2   4,943,370   +    Pf1N1B4_4481   0.90  -2.0   4,943,370   +    Pf1N1B4_4481   0.90  +1.8   4,943,370   +    Pf1N1B4_4481   0.90  -1.0   4,943,372   +        +0.4   4,943,372   +        -0.4   4,943,372   +        +1.0   4,943,372   +        +0.8   4,943,550   +        +0.6   4,943,550   +        +0.3   4,943,578   -        +1.1   4,943,809   -    Pf1N1B4_4482   0.30  +1.4   4,943,809   -    Pf1N1B4_4482   0.30  -0.2   4,943,809   -    Pf1N1B4_4482   0.30  -1.7   4,943,809   -    Pf1N1B4_4482   0.30  -0.1   4,943,958   +    Pf1N1B4_4482   0.47  +0.9   4,943,959   -    Pf1N1B4_4482   0.47  +0.6   4,943,959   -    Pf1N1B4_4482   0.47  +0.9   4,943,959   -    Pf1N1B4_4482   0.47  +1.1   4,943,978   -    Pf1N1B4_4482   0.50  +0.9   4,944,303   +    Pf1N1B4_4482   0.87  +1.3   4,944,351   +        +0.0   4,944,352   -        -0.7   4,944,360   +        +2.5   4,944,360   +        +2.5   4,944,360   +        +0.6   4,944,360   +        +2.0   4,944,361   -        -1.4   4,944,366   +        +2.1   4,944,367   -        +0.9   4,944,367   -        +0.4   4,944,465   +        +1.4   4,944,465   +        -0.7   4,944,466   -        -2.5   4,944,735   -    Pf1N1B4_4483   0.65  +0.2   4,944,757   +    Pf1N1B4_4483   0.71  +1.8   4,944,757   +    Pf1N1B4_4483   0.71  +1.0   4,944,757   +    Pf1N1B4_4483   0.71  +0.9   4,944,758   -    Pf1N1B4_4483   0.72  +0.9   4,944,758   -        -1.4   4,944,758   -    Pf1N1B4_4483   0.72  -1.6   4,944,761   +    Pf1N1B4_4483   0.73  +0.2   4,944,761   +    Pf1N1B4_4483   0.73  +1.0   4,944,761   +    Pf1N1B4_4483   0.73  -1.7   4,944,761   +    Pf1N1B4_4483   0.73  +0.9   4,944,761   +    Pf1N1B4_4483   0.73  -0.1   4,944,761   +    Pf1N1B4_4483   0.73  -2.2   4,944,761   +    Pf1N1B4_4483   0.73  -0.7   4,944,762   -    Pf1N1B4_4483   0.73  -0.6   4,944,766   +    Pf1N1B4_4483   0.74  -0.1   4,944,766   +    Pf1N1B4_4483   0.74  +1.8   4,944,767   -    Pf1N1B4_4483   0.74  -0.4   4,944,820   +        +0.6   4,944,821   -        -0.6   4,944,977   +        +2.0   4,945,021   -        +0.1   4,945,083   -        -1.0   4,945,085   +        -0.2   4,945,086   -        +0.0   4,945,180   +    Pf1N1B4_4484   0.12  -0.3   4,945,180   +    Pf1N1B4_4484   0.12  -2.4   4,945,180   +    Pf1N1B4_4484   0.12  +1.0   4,945,184   -    Pf1N1B4_4484   0.12  -0.1   4,945,220   -    Pf1N1B4_4484   0.15  -0.8   4,945,220   -    Pf1N1B4_4484   0.15  +1.6 
 
Or see this region's nucleotide sequence