Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4325 and Pf1N1B4_4326 are separated by 4 nucleotides Pf1N1B4_4326 and Pf1N1B4_4327 overlap by 29 nucleotides
Pf1N1B4_4325 - Agmatine deiminase (EC 3.5.3.12), at 4,785,715 to 4,786,767
_4325
Pf1N1B4_4326 - N-carbamoylputrescine amidase (3.5.1.53), at 4,786,772 to 4,787,680
_4326
Pf1N1B4_4327 - Spermidine/putrescine-binding periplasmic protein, at 4,787,652 to 4,788,785
_4327
Position (kb)
4786
4787
4788 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4786.150 kb on - strand, within Pf1N1B4_4325 at 4786.162 kb on - strand, within Pf1N1B4_4325 at 4786.335 kb on - strand, within Pf1N1B4_4325 at 4786.335 kb on - strand, within Pf1N1B4_4325 at 4786.433 kb on - strand, within Pf1N1B4_4325 at 4786.516 kb on + strand, within Pf1N1B4_4325 at 4786.516 kb on + strand, within Pf1N1B4_4325 at 4786.517 kb on - strand, within Pf1N1B4_4325 at 4786.517 kb on - strand at 4786.579 kb on + strand, within Pf1N1B4_4325 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.917 kb on - strand, within Pf1N1B4_4326 at 4786.980 kb on + strand at 4787.061 kb on - strand, within Pf1N1B4_4326 at 4787.159 kb on + strand, within Pf1N1B4_4326 at 4787.160 kb on - strand, within Pf1N1B4_4326 at 4787.462 kb on + strand, within Pf1N1B4_4326 at 4787.616 kb on - strand at 4787.616 kb on - strand at 4787.779 kb on - strand, within Pf1N1B4_4327 at 4787.794 kb on - strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4787.850 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.042 kb on + strand, within Pf1N1B4_4327 at 4788.043 kb on - strand at 4788.043 kb on - strand at 4788.072 kb on + strand, within Pf1N1B4_4327 at 4788.073 kb on - strand, within Pf1N1B4_4327 at 4788.099 kb on + strand at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.201 kb on - strand, within Pf1N1B4_4327 at 4788.249 kb on + strand, within Pf1N1B4_4327 at 4788.250 kb on - strand, within Pf1N1B4_4327 at 4788.320 kb on - strand, within Pf1N1B4_4327 at 4788.327 kb on + strand, within Pf1N1B4_4327 at 4788.332 kb on - strand, within Pf1N1B4_4327 at 4788.543 kb on + strand, within Pf1N1B4_4327 at 4788.544 kb on - strand, within Pf1N1B4_4327 at 4788.672 kb on + strand at 4788.672 kb on + strand at 4788.673 kb on - strand at 4788.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 4,786,150 - Pf1N1B4_4325 0.41 -1.1 4,786,162 - Pf1N1B4_4325 0.42 -1.7 4,786,335 - Pf1N1B4_4325 0.59 -0.5 4,786,335 - Pf1N1B4_4325 0.59 -1.0 4,786,433 - Pf1N1B4_4325 0.68 +0.6 4,786,516 + Pf1N1B4_4325 0.76 +0.6 4,786,516 + Pf1N1B4_4325 0.76 +0.0 4,786,517 - Pf1N1B4_4325 0.76 -2.7 4,786,517 - +2.6 4,786,579 + Pf1N1B4_4325 0.82 -0.3 4,786,917 - Pf1N1B4_4326 0.16 -0.1 4,786,917 - Pf1N1B4_4326 0.16 -1.0 4,786,917 - Pf1N1B4_4326 0.16 -2.0 4,786,980 + +0.6 4,787,061 - Pf1N1B4_4326 0.32 -1.2 4,787,159 + Pf1N1B4_4326 0.43 -0.4 4,787,160 - Pf1N1B4_4326 0.43 -1.2 4,787,462 + Pf1N1B4_4326 0.76 -0.4 4,787,616 - -0.4 4,787,616 - +1.9 4,787,779 - Pf1N1B4_4327 0.11 -1.4 4,787,794 - Pf1N1B4_4327 0.13 +0.9 4,787,850 + Pf1N1B4_4327 0.17 -1.7 4,787,850 + Pf1N1B4_4327 0.17 +0.0 4,787,850 + Pf1N1B4_4327 0.17 -1.7 4,787,850 + Pf1N1B4_4327 0.17 -1.6 4,788,042 + Pf1N1B4_4327 0.34 +0.1 4,788,042 + Pf1N1B4_4327 0.34 -1.7 4,788,042 + Pf1N1B4_4327 0.34 +0.0 4,788,042 + Pf1N1B4_4327 0.34 +0.6 4,788,043 - -0.4 4,788,043 - +1.0 4,788,072 + Pf1N1B4_4327 0.37 -0.3 4,788,073 - Pf1N1B4_4327 0.37 +0.2 4,788,099 + +0.6 4,788,201 - Pf1N1B4_4327 0.48 +0.0 4,788,201 - Pf1N1B4_4327 0.48 -0.6 4,788,249 + Pf1N1B4_4327 0.53 -3.3 4,788,250 - Pf1N1B4_4327 0.53 -2.2 4,788,320 - Pf1N1B4_4327 0.59 +1.6 4,788,327 + Pf1N1B4_4327 0.60 -2.1 4,788,332 - Pf1N1B4_4327 0.60 -1.0 4,788,543 + Pf1N1B4_4327 0.79 +1.6 4,788,544 - Pf1N1B4_4327 0.79 -0.8 4,788,672 + +0.9 4,788,672 + -1.0 4,788,673 - +1.4 4,788,673 - -2.6
Or see this region's nucleotide sequence