Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4320 and Pf1N1B4_4321 overlap by 7 nucleotides Pf1N1B4_4321 and Pf1N1B4_4322 overlap by 4 nucleotides Pf1N1B4_4322 and Pf1N1B4_4323 are separated by 15 nucleotides Pf1N1B4_4323 and Pf1N1B4_4324 overlap by 31 nucleotides
Pf1N1B4_4320 - Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1), at 4,782,101 to 4,782,928
_4320
Pf1N1B4_4321 - Phosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 4,782,922 to 4,783,719
_4321
Pf1N1B4_4322 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,783,716 to 4,784,567
_4322
Pf1N1B4_4323 - hypothetical protein, at 4,784,583 to 4,784,744
_4323
Pf1N1B4_4324 - Glycine cleavage system transcriptional activator, at 4,784,714 to 4,785,580
_4324
Position (kb)
4783
4784
4785 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4783.150 kb on - strand at 4783.235 kb on + strand, within Pf1N1B4_4321 at 4783.236 kb on - strand, within Pf1N1B4_4321 at 4783.762 kb on + strand at 4783.763 kb on - strand at 4783.862 kb on + strand, within Pf1N1B4_4322 at 4784.087 kb on - strand at 4784.087 kb on - strand, within Pf1N1B4_4322 at 4784.118 kb on - strand, within Pf1N1B4_4322 at 4784.118 kb on - strand at 4784.122 kb on + strand, within Pf1N1B4_4322 at 4784.419 kb on + strand, within Pf1N1B4_4322 at 4784.582 kb on + strand at 4784.582 kb on + strand at 4784.715 kb on + strand at 4784.715 kb on + strand at 4784.716 kb on - strand at 4784.716 kb on - strand at 4784.716 kb on - strand at 4784.879 kb on + strand at 4784.972 kb on + strand, within Pf1N1B4_4324 at 4785.160 kb on + strand, within Pf1N1B4_4324 at 4785.206 kb on + strand at 4785.206 kb on + strand at 4785.441 kb on + strand, within Pf1N1B4_4324
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 4,783,150 - +1.2 4,783,235 + Pf1N1B4_4321 0.39 -0.1 4,783,236 - Pf1N1B4_4321 0.39 -0.6 4,783,762 + -1.0 4,783,763 - +0.0 4,783,862 + Pf1N1B4_4322 0.17 +0.4 4,784,087 - -1.7 4,784,087 - Pf1N1B4_4322 0.44 +1.1 4,784,118 - Pf1N1B4_4322 0.47 +0.4 4,784,118 - +0.6 4,784,122 + Pf1N1B4_4322 0.48 +0.6 4,784,419 + Pf1N1B4_4322 0.83 +0.6 4,784,582 + +0.4 4,784,582 + -0.4 4,784,715 + +1.5 4,784,715 + -1.7 4,784,716 - +2.2 4,784,716 - +0.5 4,784,716 - -0.1 4,784,879 + -1.0 4,784,972 + Pf1N1B4_4324 0.30 +0.6 4,785,160 + Pf1N1B4_4324 0.51 -2.7 4,785,206 + -1.0 4,785,206 + -0.4 4,785,441 + Pf1N1B4_4324 0.84 +0.1
Or see this region's nucleotide sequence