Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_413 and Pf1N1B4_414 are separated by 60 nucleotides Pf1N1B4_414 and Pf1N1B4_415 are separated by 242 nucleotides
Pf1N1B4_413 - L-arabinolactonase (EC 3.1.1.15) (from data) , at 482,629 to 483,573
_413
Pf1N1B4_414 - transcriptional regulator, at 483,634 to 484,350
_414
Pf1N1B4_415 - Lipase precursor (EC 3.1.1.3), at 484,593 to 485,471
_415
Position (kb)
483
484
485 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.668 kb on + strand at 482.669 kb on - strand at 482.669 kb on - strand at 482.732 kb on + strand, within Pf1N1B4_413 at 482.732 kb on + strand, within Pf1N1B4_413 at 482.732 kb on + strand, within Pf1N1B4_413 at 482.732 kb on + strand, within Pf1N1B4_413 at 482.733 kb on - strand, within Pf1N1B4_413 at 482.810 kb on + strand, within Pf1N1B4_413 at 482.811 kb on - strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand, within Pf1N1B4_413 at 482.863 kb on + strand at 482.863 kb on + strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.864 kb on - strand, within Pf1N1B4_413 at 482.867 kb on + strand, within Pf1N1B4_413 at 482.867 kb on + strand, within Pf1N1B4_413 at 482.867 kb on + strand, within Pf1N1B4_413 at 482.867 kb on + strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.868 kb on - strand, within Pf1N1B4_413 at 482.930 kb on + strand, within Pf1N1B4_413 at 482.930 kb on + strand, within Pf1N1B4_413 at 482.930 kb on + strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 482.931 kb on - strand, within Pf1N1B4_413 at 483.002 kb on + strand, within Pf1N1B4_413 at 483.003 kb on - strand, within Pf1N1B4_413 at 483.049 kb on + strand, within Pf1N1B4_413 at 483.049 kb on + strand, within Pf1N1B4_413 at 483.050 kb on - strand, within Pf1N1B4_413 at 483.058 kb on + strand, within Pf1N1B4_413 at 483.058 kb on + strand, within Pf1N1B4_413 at 483.059 kb on - strand, within Pf1N1B4_413 at 483.059 kb on - strand, within Pf1N1B4_413 at 483.059 kb on - strand, within Pf1N1B4_413 at 483.061 kb on + strand, within Pf1N1B4_413 at 483.124 kb on + strand, within Pf1N1B4_413 at 483.418 kb on + strand, within Pf1N1B4_413 at 483.418 kb on + strand, within Pf1N1B4_413 at 483.419 kb on - strand, within Pf1N1B4_413 at 483.419 kb on - strand at 483.519 kb on + strand at 483.520 kb on - strand at 483.528 kb on + strand at 483.529 kb on - strand at 483.529 kb on - strand at 483.529 kb on - strand at 483.635 kb on + strand at 483.636 kb on - strand at 483.926 kb on + strand, within Pf1N1B4_414 at 483.965 kb on + strand, within Pf1N1B4_414 at 483.965 kb on + strand, within Pf1N1B4_414 at 483.966 kb on - strand, within Pf1N1B4_414 at 483.966 kb on - strand at 483.966 kb on - strand at 483.966 kb on - strand, within Pf1N1B4_414 at 484.148 kb on - strand, within Pf1N1B4_414 at 484.523 kb on - strand at 484.523 kb on - strand at 484.527 kb on + strand at 484.527 kb on + strand at 484.527 kb on + strand at 484.528 kb on - strand at 484.588 kb on + strand at 484.588 kb on + strand at 484.589 kb on - strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.592 kb on + strand at 484.593 kb on - strand at 484.617 kb on - strand at 484.624 kb on - strand at 484.624 kb on - strand at 484.680 kb on + strand at 484.870 kb on - strand, within Pf1N1B4_415 at 484.874 kb on - strand at 484.996 kb on + strand, within Pf1N1B4_415 at 485.124 kb on + strand, within Pf1N1B4_415 at 485.124 kb on + strand, within Pf1N1B4_415 at 485.125 kb on - strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand, within Pf1N1B4_415 at 485.184 kb on + strand at 485.185 kb on - strand, within Pf1N1B4_415 at 485.185 kb on - strand, within Pf1N1B4_415 at 485.185 kb on - strand, within Pf1N1B4_415 at 485.187 kb on + strand at 485.187 kb on + strand, within Pf1N1B4_415 at 485.187 kb on + strand, within Pf1N1B4_415 at 485.187 kb on + strand, within Pf1N1B4_415 at 485.187 kb on + strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.188 kb on - strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.190 kb on + strand, within Pf1N1B4_415 at 485.191 kb on - strand at 485.209 kb on + strand, within Pf1N1B4_415 at 485.258 kb on + strand, within Pf1N1B4_415 at 485.259 kb on - strand, within Pf1N1B4_415 at 485.259 kb on - strand, within Pf1N1B4_415 at 485.280 kb on + strand, within Pf1N1B4_415 at 485.280 kb on + strand, within Pf1N1B4_415 at 485.280 kb on + strand at 485.319 kb on + strand, within Pf1N1B4_415
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 482,668 + -0.1 482,668 + -0.1 482,668 + -0.7 482,668 + -0.4 482,668 + +0.2 482,668 + +0.1 482,668 + -0.1 482,669 - -1.5 482,669 - +1.0 482,732 + Pf1N1B4_413 0.11 +0.5 482,732 + Pf1N1B4_413 0.11 +0.5 482,732 + Pf1N1B4_413 0.11 -0.7 482,732 + Pf1N1B4_413 0.11 +0.2 482,733 - Pf1N1B4_413 0.11 -0.7 482,810 + Pf1N1B4_413 0.19 -0.1 482,811 - Pf1N1B4_413 0.19 -0.1 482,863 + Pf1N1B4_413 0.25 -1.4 482,863 + Pf1N1B4_413 0.25 +0.2 482,863 + Pf1N1B4_413 0.25 -1.1 482,863 + Pf1N1B4_413 0.25 -0.9 482,863 + Pf1N1B4_413 0.25 -0.1 482,863 + Pf1N1B4_413 0.25 -0.2 482,863 + +0.9 482,863 + Pf1N1B4_413 0.25 -0.3 482,864 - Pf1N1B4_413 0.25 +0.5 482,864 - Pf1N1B4_413 0.25 -0.9 482,864 - Pf1N1B4_413 0.25 -0.1 482,864 - Pf1N1B4_413 0.25 -1.1 482,864 - Pf1N1B4_413 0.25 -2.1 482,864 - Pf1N1B4_413 0.25 -1.7 482,864 - Pf1N1B4_413 0.25 -0.7 482,864 - Pf1N1B4_413 0.25 +0.5 482,864 - Pf1N1B4_413 0.25 +1.3 482,867 + Pf1N1B4_413 0.25 +0.3 482,867 + Pf1N1B4_413 0.25 -1.8 482,867 + Pf1N1B4_413 0.25 -1.0 482,867 + Pf1N1B4_413 0.25 -1.1 482,868 - Pf1N1B4_413 0.25 -2.0 482,868 - Pf1N1B4_413 0.25 -0.3 482,868 - Pf1N1B4_413 0.25 +0.9 482,868 - Pf1N1B4_413 0.25 +0.3 482,868 - Pf1N1B4_413 0.25 -1.1 482,868 - Pf1N1B4_413 0.25 +0.5 482,868 - Pf1N1B4_413 0.25 +0.9 482,930 + Pf1N1B4_413 0.32 -1.9 482,930 + Pf1N1B4_413 0.32 +1.3 482,930 + Pf1N1B4_413 0.32 -1.9 482,931 - Pf1N1B4_413 0.32 +0.6 482,931 - Pf1N1B4_413 0.32 -1.8 482,931 - Pf1N1B4_413 0.32 +0.5 482,931 - Pf1N1B4_413 0.32 -0.7 482,931 - Pf1N1B4_413 0.32 -0.0 482,931 - Pf1N1B4_413 0.32 -0.1 483,002 + Pf1N1B4_413 0.39 -1.7 483,003 - Pf1N1B4_413 0.40 -2.4 483,049 + Pf1N1B4_413 0.44 -0.3 483,049 + Pf1N1B4_413 0.44 +0.6 483,050 - Pf1N1B4_413 0.45 +0.5 483,058 + Pf1N1B4_413 0.45 -0.1 483,058 + Pf1N1B4_413 0.45 -1.1 483,059 - Pf1N1B4_413 0.46 -0.8 483,059 - Pf1N1B4_413 0.46 +0.9 483,059 - Pf1N1B4_413 0.46 -2.1 483,061 + Pf1N1B4_413 0.46 -1.9 483,124 + Pf1N1B4_413 0.52 +0.2 483,418 + Pf1N1B4_413 0.83 +0.4 483,418 + Pf1N1B4_413 0.83 +0.9 483,419 - Pf1N1B4_413 0.84 +0.6 483,419 - -0.7 483,519 + +0.2 483,520 - +0.9 483,528 + -2.5 483,529 - -1.4 483,529 - -0.4 483,529 - -1.4 483,635 + -2.7 483,636 - +0.2 483,926 + Pf1N1B4_414 0.41 -0.7 483,965 + Pf1N1B4_414 0.46 -1.2 483,965 + Pf1N1B4_414 0.46 -0.4 483,966 - Pf1N1B4_414 0.46 -0.5 483,966 - +0.9 483,966 - -0.7 483,966 - Pf1N1B4_414 0.46 -1.2 484,148 - Pf1N1B4_414 0.72 -1.4 484,523 - -0.7 484,523 - -0.3 484,527 + +0.9 484,527 + +0.9 484,527 + +1.2 484,528 - +0.3 484,588 + +0.4 484,588 + -0.9 484,589 - -0.7 484,592 + -1.2 484,592 + -0.4 484,592 + -0.2 484,592 + -0.6 484,592 + +0.2 484,593 - -0.1 484,617 - -0.0 484,624 - -0.1 484,624 - -1.1 484,680 + -0.9 484,870 - Pf1N1B4_415 0.32 +0.0 484,874 - +0.3 484,996 + Pf1N1B4_415 0.46 -0.7 485,124 + Pf1N1B4_415 0.60 -0.1 485,124 + Pf1N1B4_415 0.60 +1.1 485,125 - Pf1N1B4_415 0.61 -0.3 485,184 + Pf1N1B4_415 0.67 -0.7 485,184 + -0.7 485,184 + Pf1N1B4_415 0.67 -0.1 485,184 + Pf1N1B4_415 0.67 -0.1 485,184 + Pf1N1B4_415 0.67 +1.3 485,184 + Pf1N1B4_415 0.67 -0.1 485,184 + Pf1N1B4_415 0.67 +2.5 485,184 + -0.7 485,185 - Pf1N1B4_415 0.67 +3.3 485,185 - Pf1N1B4_415 0.67 -1.9 485,185 - Pf1N1B4_415 0.67 -2.7 485,187 + -0.1 485,187 + Pf1N1B4_415 0.68 +0.9 485,187 + Pf1N1B4_415 0.68 -2.4 485,187 + Pf1N1B4_415 0.68 +1.8 485,187 + Pf1N1B4_415 0.68 -2.6 485,188 - Pf1N1B4_415 0.68 +0.7 485,188 - Pf1N1B4_415 0.68 -1.4 485,188 - Pf1N1B4_415 0.68 -1.9 485,188 - Pf1N1B4_415 0.68 +0.1 485,188 - Pf1N1B4_415 0.68 +0.0 485,188 - Pf1N1B4_415 0.68 +0.3 485,190 + Pf1N1B4_415 0.68 -1.1 485,190 + Pf1N1B4_415 0.68 +0.7 485,190 + Pf1N1B4_415 0.68 +0.3 485,190 + Pf1N1B4_415 0.68 +0.5 485,191 - +0.9 485,209 + Pf1N1B4_415 0.70 +0.2 485,258 + Pf1N1B4_415 0.76 +0.5 485,259 - Pf1N1B4_415 0.76 +0.9 485,259 - Pf1N1B4_415 0.76 -0.4 485,280 + Pf1N1B4_415 0.78 +0.6 485,280 + Pf1N1B4_415 0.78 +1.1 485,280 + +0.9 485,319 + Pf1N1B4_415 0.83 +0.3
Or see this region's nucleotide sequence