Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4064 and Pf1N1B4_4065 are separated by 0 nucleotides Pf1N1B4_4065 and Pf1N1B4_4066 are separated by 139 nucleotides
Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289
_4064
Pf1N1B4_4065 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 4,519,290 to 4,520,684
_4065
Pf1N1B4_4066 - Glutathione S-transferase, omega (EC 2.5.1.18), at 4,520,824 to 4,521,828
_4066
Position (kb)
4519
4520
4521 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4519.287 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.296 kb on + strand at 4519.297 kb on - strand at 4519.333 kb on + strand at 4519.333 kb on + strand at 4519.408 kb on + strand at 4519.408 kb on + strand at 4519.741 kb on - strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.834 kb on + strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4519.835 kb on - strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.028 kb on + strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.029 kb on - strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.088 kb on + strand, within Pf1N1B4_4065 at 4520.204 kb on + strand, within Pf1N1B4_4065 at 4520.205 kb on - strand, within Pf1N1B4_4065 at 4520.304 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.433 kb on + strand, within Pf1N1B4_4065 at 4520.434 kb on - strand, within Pf1N1B4_4065 at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.596 kb on + strand at 4520.597 kb on - strand at 4520.597 kb on - strand at 4520.597 kb on - strand at 4520.727 kb on - strand at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.949 kb on - strand, within Pf1N1B4_4066 at 4520.951 kb on + strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4520.952 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.006 kb on - strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.080 kb on + strand, within Pf1N1B4_4066 at 4521.081 kb on - strand, within Pf1N1B4_4066 at 4521.200 kb on - strand at 4521.221 kb on + strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.222 kb on - strand, within Pf1N1B4_4066 at 4521.479 kb on + strand, within Pf1N1B4_4066 at 4521.662 kb on + strand at 4521.663 kb on - strand, within Pf1N1B4_4066
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 4,519,287 + +0.6 4,519,296 + -0.3 4,519,296 + +0.2 4,519,296 + +1.1 4,519,297 - -1.1 4,519,333 + -3.1 4,519,333 + -0.1 4,519,408 + +0.7 4,519,408 + +0.7 4,519,741 - Pf1N1B4_4065 0.32 +1.1 4,519,834 + Pf1N1B4_4065 0.39 -0.4 4,519,834 + Pf1N1B4_4065 0.39 +1.5 4,519,834 + +2.3 4,519,834 + Pf1N1B4_4065 0.39 +1.1 4,519,834 + Pf1N1B4_4065 0.39 +0.6 4,519,834 + Pf1N1B4_4065 0.39 +1.7 4,519,834 + Pf1N1B4_4065 0.39 +0.7 4,519,835 - Pf1N1B4_4065 0.39 -1.9 4,519,835 - Pf1N1B4_4065 0.39 +1.3 4,519,835 - Pf1N1B4_4065 0.39 -0.2 4,519,835 - Pf1N1B4_4065 0.39 -0.5 4,519,835 - Pf1N1B4_4065 0.39 -3.3 4,520,028 + Pf1N1B4_4065 0.53 +0.0 4,520,028 + Pf1N1B4_4065 0.53 +0.4 4,520,028 + Pf1N1B4_4065 0.53 -0.6 4,520,028 + Pf1N1B4_4065 0.53 -3.0 4,520,028 + Pf1N1B4_4065 0.53 +0.4 4,520,028 + Pf1N1B4_4065 0.53 +1.1 4,520,029 - Pf1N1B4_4065 0.53 -0.5 4,520,029 - Pf1N1B4_4065 0.53 +0.7 4,520,088 + Pf1N1B4_4065 0.57 +0.7 4,520,088 + Pf1N1B4_4065 0.57 -1.6 4,520,088 + Pf1N1B4_4065 0.57 +0.2 4,520,088 + Pf1N1B4_4065 0.57 +0.8 4,520,088 + Pf1N1B4_4065 0.57 -3.0 4,520,204 + Pf1N1B4_4065 0.66 -0.1 4,520,205 - Pf1N1B4_4065 0.66 -1.9 4,520,304 + Pf1N1B4_4065 0.73 -1.3 4,520,433 + Pf1N1B4_4065 0.82 +0.7 4,520,433 + Pf1N1B4_4065 0.82 +0.2 4,520,433 + Pf1N1B4_4065 0.82 +0.5 4,520,433 + Pf1N1B4_4065 0.82 -0.6 4,520,433 + Pf1N1B4_4065 0.82 +0.7 4,520,433 + Pf1N1B4_4065 0.82 -1.6 4,520,434 - Pf1N1B4_4065 0.82 +1.1 4,520,596 + -2.3 4,520,596 + -0.1 4,520,596 + -0.3 4,520,596 + +1.5 4,520,596 + +0.6 4,520,596 + +0.7 4,520,596 + +1.3 4,520,596 + +1.6 4,520,597 - +1.0 4,520,597 - +1.4 4,520,597 - -0.3 4,520,727 - -0.5 4,520,949 - Pf1N1B4_4066 0.12 +0.3 4,520,949 - Pf1N1B4_4066 0.12 +0.1 4,520,951 + Pf1N1B4_4066 0.13 -1.9 4,520,952 - Pf1N1B4_4066 0.13 +0.7 4,520,952 - Pf1N1B4_4066 0.13 -0.3 4,520,952 - Pf1N1B4_4066 0.13 -0.1 4,520,952 - Pf1N1B4_4066 0.13 +1.4 4,521,006 - Pf1N1B4_4066 0.18 +0.7 4,521,006 - Pf1N1B4_4066 0.18 -1.3 4,521,080 + Pf1N1B4_4066 0.25 -1.3 4,521,080 + Pf1N1B4_4066 0.25 -0.5 4,521,080 + Pf1N1B4_4066 0.25 -0.6 4,521,081 - Pf1N1B4_4066 0.26 +0.0 4,521,200 - -1.3 4,521,221 + Pf1N1B4_4066 0.40 -0.3 4,521,222 - Pf1N1B4_4066 0.40 -0.6 4,521,222 - Pf1N1B4_4066 0.40 -2.3 4,521,479 + Pf1N1B4_4066 0.65 -0.0 4,521,662 + -1.9 4,521,663 - Pf1N1B4_4066 0.83 -1.2
Or see this region's nucleotide sequence