Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3359 and Pf1N1B4_3360 are separated by 13 nucleotides Pf1N1B4_3360 and Pf1N1B4_3361 are separated by 125 nucleotides Pf1N1B4_3361 and Pf1N1B4_3362 are separated by 13 nucleotides
Pf1N1B4_3359 - Glutamine amidotransferase, class-II, at 3,745,118 to 3,745,894
_3359
Pf1N1B4_3360 - FIG00953701: hypothetical protein, at 3,745,908 to 3,746,450
_3360
Pf1N1B4_3361 - Outer membrane receptor proteins, likely involved in siderophore uptake @ TonB-dependent siderophore receptor, at 3,746,576 to 3,748,687
_3361
Pf1N1B4_3362 - Multidrug resistance protein B, at 3,748,701 to 3,749,912
_3362
Position (kb)
3746
3747
3748
3749 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3745.920 kb on + strand at 3745.920 kb on + strand at 3745.921 kb on - strand at 3745.921 kb on - strand at 3745.921 kb on - strand at 3745.990 kb on - strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.078 kb on + strand, within Pf1N1B4_3360 at 3746.079 kb on - strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.211 kb on + strand, within Pf1N1B4_3360 at 3746.212 kb on - strand, within Pf1N1B4_3360 at 3746.277 kb on + strand, within Pf1N1B4_3360 at 3746.277 kb on + strand, within Pf1N1B4_3360 at 3746.448 kb on + strand at 3746.501 kb on + strand at 3746.534 kb on + strand at 3746.534 kb on + strand at 3746.534 kb on + strand at 3746.535 kb on - strand at 3746.535 kb on - strand at 3746.578 kb on - strand at 3746.668 kb on - strand at 3746.689 kb on - strand at 3746.689 kb on - strand at 3746.689 kb on - strand at 3746.689 kb on - strand at 3746.749 kb on + strand at 3746.749 kb on + strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.750 kb on - strand at 3746.752 kb on - strand at 3746.796 kb on + strand, within Pf1N1B4_3361 at 3746.832 kb on + strand, within Pf1N1B4_3361 at 3746.833 kb on - strand, within Pf1N1B4_3361 at 3746.847 kb on + strand, within Pf1N1B4_3361 at 3746.943 kb on + strand, within Pf1N1B4_3361 at 3746.943 kb on + strand, within Pf1N1B4_3361 at 3746.943 kb on + strand, within Pf1N1B4_3361 at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3746.944 kb on - strand at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3746.944 kb on - strand at 3746.944 kb on - strand, within Pf1N1B4_3361 at 3747.055 kb on - strand, within Pf1N1B4_3361 at 3747.055 kb on - strand, within Pf1N1B4_3361 at 3747.055 kb on - strand, within Pf1N1B4_3361 at 3747.084 kb on + strand, within Pf1N1B4_3361 at 3747.084 kb on + strand, within Pf1N1B4_3361 at 3747.244 kb on - strand, within Pf1N1B4_3361 at 3747.244 kb on - strand, within Pf1N1B4_3361 at 3747.297 kb on + strand, within Pf1N1B4_3361 at 3747.297 kb on + strand, within Pf1N1B4_3361 at 3747.298 kb on - strand, within Pf1N1B4_3361 at 3747.312 kb on + strand, within Pf1N1B4_3361 at 3747.313 kb on - strand, within Pf1N1B4_3361 at 3747.370 kb on - strand, within Pf1N1B4_3361 at 3747.504 kb on + strand, within Pf1N1B4_3361 at 3747.504 kb on + strand, within Pf1N1B4_3361 at 3747.525 kb on + strand, within Pf1N1B4_3361 at 3747.525 kb on + strand, within Pf1N1B4_3361 at 3747.525 kb on + strand, within Pf1N1B4_3361 at 3747.526 kb on - strand, within Pf1N1B4_3361 at 3747.582 kb on + strand at 3747.582 kb on + strand, within Pf1N1B4_3361 at 3747.583 kb on - strand, within Pf1N1B4_3361 at 3747.747 kb on + strand, within Pf1N1B4_3361 at 3747.748 kb on - strand, within Pf1N1B4_3361 at 3747.748 kb on - strand, within Pf1N1B4_3361 at 3747.774 kb on + strand, within Pf1N1B4_3361 at 3747.774 kb on + strand, within Pf1N1B4_3361 at 3747.775 kb on - strand, within Pf1N1B4_3361 at 3747.775 kb on - strand, within Pf1N1B4_3361 at 3747.792 kb on + strand, within Pf1N1B4_3361 at 3747.792 kb on + strand at 3747.793 kb on - strand, within Pf1N1B4_3361 at 3747.820 kb on + strand, within Pf1N1B4_3361 at 3747.822 kb on + strand, within Pf1N1B4_3361 at 3747.822 kb on + strand, within Pf1N1B4_3361 at 3747.915 kb on + strand, within Pf1N1B4_3361 at 3747.916 kb on - strand, within Pf1N1B4_3361 at 3747.916 kb on - strand, within Pf1N1B4_3361 at 3747.916 kb on - strand, within Pf1N1B4_3361 at 3747.916 kb on - strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.984 kb on + strand, within Pf1N1B4_3361 at 3747.985 kb on - strand, within Pf1N1B4_3361 at 3748.050 kb on + strand, within Pf1N1B4_3361 at 3748.086 kb on + strand, within Pf1N1B4_3361 at 3748.087 kb on - strand, within Pf1N1B4_3361 at 3748.087 kb on - strand, within Pf1N1B4_3361 at 3748.087 kb on - strand, within Pf1N1B4_3361 at 3748.191 kb on + strand, within Pf1N1B4_3361 at 3748.192 kb on - strand at 3748.192 kb on - strand, within Pf1N1B4_3361 at 3748.192 kb on - strand, within Pf1N1B4_3361 at 3748.192 kb on - strand, within Pf1N1B4_3361 at 3748.192 kb on - strand at 3748.192 kb on - strand at 3748.192 kb on - strand, within Pf1N1B4_3361 at 3748.213 kb on + strand, within Pf1N1B4_3361 at 3748.214 kb on - strand, within Pf1N1B4_3361 at 3748.260 kb on + strand, within Pf1N1B4_3361 at 3748.260 kb on + strand, within Pf1N1B4_3361 at 3748.260 kb on + strand, within Pf1N1B4_3361 at 3748.332 kb on + strand, within Pf1N1B4_3361 at 3748.332 kb on + strand, within Pf1N1B4_3361 at 3748.333 kb on - strand, within Pf1N1B4_3361 at 3748.358 kb on + strand, within Pf1N1B4_3361 at 3748.702 kb on + strand at 3748.702 kb on + strand at 3748.703 kb on - strand at 3748.711 kb on + strand at 3748.711 kb on + strand at 3748.712 kb on - strand at 3748.712 kb on - strand at 3748.792 kb on + strand at 3748.792 kb on + strand at 3748.792 kb on + strand at 3748.792 kb on + strand at 3748.793 kb on - strand at 3748.793 kb on - strand at 3748.793 kb on - strand at 3749.218 kb on + strand at 3749.219 kb on - strand, within Pf1N1B4_3362 at 3749.219 kb on - strand, within Pf1N1B4_3362 at 3749.343 kb on - strand, within Pf1N1B4_3362 at 3749.545 kb on + strand, within Pf1N1B4_3362 at 3749.546 kb on - strand, within Pf1N1B4_3362 at 3749.546 kb on - strand, within Pf1N1B4_3362 at 3749.685 kb on - strand, within Pf1N1B4_3362
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 3,745,920 + -0.5 3,745,920 + -3.1 3,745,921 - +0.1 3,745,921 - -1.0 3,745,921 - -0.0 3,745,990 - Pf1N1B4_3360 0.15 -0.5 3,746,078 + Pf1N1B4_3360 0.31 +1.1 3,746,078 + Pf1N1B4_3360 0.31 +0.3 3,746,078 + Pf1N1B4_3360 0.31 -0.6 3,746,078 + Pf1N1B4_3360 0.31 +0.1 3,746,079 - Pf1N1B4_3360 0.31 -0.1 3,746,211 + Pf1N1B4_3360 0.56 -0.0 3,746,211 + Pf1N1B4_3360 0.56 -1.4 3,746,211 + Pf1N1B4_3360 0.56 +1.5 3,746,211 + Pf1N1B4_3360 0.56 -0.9 3,746,211 + Pf1N1B4_3360 0.56 +0.7 3,746,212 - Pf1N1B4_3360 0.56 +0.5 3,746,277 + Pf1N1B4_3360 0.68 -0.6 3,746,277 + Pf1N1B4_3360 0.68 -0.3 3,746,448 + +1.4 3,746,501 + +2.5 3,746,534 + +0.3 3,746,534 + +1.8 3,746,534 + -0.6 3,746,535 - -0.0 3,746,535 - +2.0 3,746,578 - +0.1 3,746,668 - +2.0 3,746,689 - -0.9 3,746,689 - -1.0 3,746,689 - -2.0 3,746,689 - +0.6 3,746,749 + +0.1 3,746,749 + -1.0 3,746,750 - -1.9 3,746,750 - -1.0 3,746,750 - -0.4 3,746,750 - -0.5 3,746,750 - -1.9 3,746,752 - +1.7 3,746,796 + Pf1N1B4_3361 0.10 -2.5 3,746,832 + Pf1N1B4_3361 0.12 -1.7 3,746,833 - Pf1N1B4_3361 0.12 -1.0 3,746,847 + Pf1N1B4_3361 0.13 -1.9 3,746,943 + Pf1N1B4_3361 0.17 -0.0 3,746,943 + Pf1N1B4_3361 0.17 -3.6 3,746,943 + Pf1N1B4_3361 0.17 +0.6 3,746,944 - Pf1N1B4_3361 0.17 -1.6 3,746,944 - -0.0 3,746,944 - Pf1N1B4_3361 0.17 -1.4 3,746,944 - Pf1N1B4_3361 0.17 -3.9 3,746,944 - -0.0 3,746,944 - Pf1N1B4_3361 0.17 +2.5 3,747,055 - Pf1N1B4_3361 0.23 -2.5 3,747,055 - Pf1N1B4_3361 0.23 -1.4 3,747,055 - Pf1N1B4_3361 0.23 -0.2 3,747,084 + Pf1N1B4_3361 0.24 -0.4 3,747,084 + Pf1N1B4_3361 0.24 -0.2 3,747,244 - Pf1N1B4_3361 0.32 -0.6 3,747,244 - Pf1N1B4_3361 0.32 -0.9 3,747,297 + Pf1N1B4_3361 0.34 -0.3 3,747,297 + Pf1N1B4_3361 0.34 -2.2 3,747,298 - Pf1N1B4_3361 0.34 -0.6 3,747,312 + Pf1N1B4_3361 0.35 -2.0 3,747,313 - Pf1N1B4_3361 0.35 -1.9 3,747,370 - Pf1N1B4_3361 0.38 -1.0 3,747,504 + Pf1N1B4_3361 0.44 -1.6 3,747,504 + Pf1N1B4_3361 0.44 -1.0 3,747,525 + Pf1N1B4_3361 0.45 -1.0 3,747,525 + Pf1N1B4_3361 0.45 -0.0 3,747,525 + Pf1N1B4_3361 0.45 -1.0 3,747,526 - Pf1N1B4_3361 0.45 -3.5 3,747,582 + +1.5 3,747,582 + Pf1N1B4_3361 0.48 -0.6 3,747,583 - Pf1N1B4_3361 0.48 -2.2 3,747,747 + Pf1N1B4_3361 0.55 -0.0 3,747,748 - Pf1N1B4_3361 0.55 -1.1 3,747,748 - Pf1N1B4_3361 0.55 -2.4 3,747,774 + Pf1N1B4_3361 0.57 +1.0 3,747,774 + Pf1N1B4_3361 0.57 -1.0 3,747,775 - Pf1N1B4_3361 0.57 -1.6 3,747,775 - Pf1N1B4_3361 0.57 -1.6 3,747,792 + Pf1N1B4_3361 0.58 -1.3 3,747,792 + +1.0 3,747,793 - Pf1N1B4_3361 0.58 -0.0 3,747,820 + Pf1N1B4_3361 0.59 -0.2 3,747,822 + Pf1N1B4_3361 0.59 -1.4 3,747,822 + Pf1N1B4_3361 0.59 -0.6 3,747,915 + Pf1N1B4_3361 0.63 -1.1 3,747,916 - Pf1N1B4_3361 0.63 -2.2 3,747,916 - Pf1N1B4_3361 0.63 -0.8 3,747,916 - Pf1N1B4_3361 0.63 -1.1 3,747,916 - Pf1N1B4_3361 0.63 -2.4 3,747,984 + Pf1N1B4_3361 0.67 -1.0 3,747,984 + Pf1N1B4_3361 0.67 -0.3 3,747,984 + -1.4 3,747,984 + Pf1N1B4_3361 0.67 -2.0 3,747,984 + Pf1N1B4_3361 0.67 +1.4 3,747,984 + Pf1N1B4_3361 0.67 -2.2 3,747,984 + Pf1N1B4_3361 0.67 -2.4 3,747,985 - Pf1N1B4_3361 0.67 -0.0 3,748,050 + Pf1N1B4_3361 0.70 -2.6 3,748,086 + Pf1N1B4_3361 0.71 +0.4 3,748,087 - Pf1N1B4_3361 0.72 +1.0 3,748,087 - Pf1N1B4_3361 0.72 -2.1 3,748,087 - Pf1N1B4_3361 0.72 +2.0 3,748,191 + Pf1N1B4_3361 0.76 -2.4 3,748,192 - -1.0 3,748,192 - Pf1N1B4_3361 0.77 -1.6 3,748,192 - Pf1N1B4_3361 0.77 -1.6 3,748,192 - Pf1N1B4_3361 0.77 -1.4 3,748,192 - +0.4 3,748,192 - -0.0 3,748,192 - Pf1N1B4_3361 0.77 -2.2 3,748,213 + Pf1N1B4_3361 0.78 -1.0 3,748,214 - Pf1N1B4_3361 0.78 -0.5 3,748,260 + Pf1N1B4_3361 0.80 -0.3 3,748,260 + Pf1N1B4_3361 0.80 +0.2 3,748,260 + Pf1N1B4_3361 0.80 -0.6 3,748,332 + Pf1N1B4_3361 0.83 -1.4 3,748,332 + Pf1N1B4_3361 0.83 -0.1 3,748,333 - Pf1N1B4_3361 0.83 +0.1 3,748,358 + Pf1N1B4_3361 0.84 -1.0 3,748,702 + -1.4 3,748,702 + -1.4 3,748,703 - -1.9 3,748,711 + +0.5 3,748,711 + -2.2 3,748,712 - +1.8 3,748,712 - +1.0 3,748,792 + -2.6 3,748,792 + -1.4 3,748,792 + -1.6 3,748,792 + -2.4 3,748,793 - -1.9 3,748,793 - +0.4 3,748,793 - -2.9 3,749,218 + -0.6 3,749,219 - Pf1N1B4_3362 0.43 -0.5 3,749,219 - Pf1N1B4_3362 0.43 -1.6 3,749,343 - Pf1N1B4_3362 0.53 -2.0 3,749,545 + Pf1N1B4_3362 0.70 -2.0 3,749,546 - Pf1N1B4_3362 0.70 -1.6 3,749,546 - Pf1N1B4_3362 0.70 -1.6 3,749,685 - Pf1N1B4_3362 0.81 -1.4
Or see this region's nucleotide sequence