Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_28 and Pf1N1B4_29 overlap by 4 nucleotides Pf1N1B4_29 and Pf1N1B4_5164 are separated by 225 nucleotides Pf1N1B4_5164 and Pf1N1B4_30 are separated by 102 nucleotides Pf1N1B4_30 and Pf1N1B4_31 overlap by 4 nucleotides
Pf1N1B4_28 - FIG00958186: hypothetical protein, at 31,985 to 32,185
_28
Pf1N1B4_29 - FIG00967200: hypothetical protein, at 32,182 to 32,412
_29
Pf1N1B4_5164 - tRNA-Asn-GTT, at 32,638 to 32,710
_5164
Pf1N1B4_30 - Cation efflux system protein, at 32,813 to 35,899
_30
Pf1N1B4_31 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 35,896 to 37,017
_31
Position (kb)
32
33
34
35
36 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 31.892 kb on - strand at 31.952 kb on + strand at 31.952 kb on + strand at 31.958 kb on + strand at 31.958 kb on + strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 31.959 kb on - strand at 32.001 kb on + strand at 32.002 kb on - strand at 32.144 kb on + strand, within Pf1N1B4_28 at 32.170 kb on + strand at 32.171 kb on - strand at 32.171 kb on - strand at 32.223 kb on - strand, within Pf1N1B4_29 at 32.223 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.232 kb on - strand, within Pf1N1B4_29 at 32.463 kb on + strand at 32.517 kb on + strand at 32.526 kb on - strand at 32.811 kb on + strand at 32.844 kb on + strand at 32.845 kb on - strand at 32.880 kb on + strand at 32.880 kb on + strand at 32.880 kb on + strand at 32.881 kb on - strand at 32.881 kb on - strand at 32.881 kb on - strand at 32.943 kb on + strand at 32.944 kb on - strand at 33.271 kb on + strand at 33.272 kb on - strand at 33.275 kb on - strand at 33.275 kb on - strand, within Pf1N1B4_30 at 33.639 kb on - strand, within Pf1N1B4_30 at 33.639 kb on - strand, within Pf1N1B4_30 at 33.655 kb on + strand, within Pf1N1B4_30 at 33.916 kb on - strand, within Pf1N1B4_30 at 34.061 kb on + strand, within Pf1N1B4_30 at 34.083 kb on + strand, within Pf1N1B4_30 at 34.084 kb on - strand, within Pf1N1B4_30 at 34.084 kb on - strand, within Pf1N1B4_30 at 34.110 kb on + strand, within Pf1N1B4_30 at 34.111 kb on - strand, within Pf1N1B4_30 at 34.187 kb on + strand, within Pf1N1B4_30 at 34.188 kb on - strand at 34.188 kb on - strand, within Pf1N1B4_30 at 34.188 kb on - strand, within Pf1N1B4_30 at 34.190 kb on - strand, within Pf1N1B4_30 at 34.368 kb on + strand, within Pf1N1B4_30 at 34.368 kb on + strand, within Pf1N1B4_30 at 34.369 kb on - strand, within Pf1N1B4_30 at 34.384 kb on - strand, within Pf1N1B4_30 at 34.451 kb on - strand, within Pf1N1B4_30 at 34.451 kb on - strand, within Pf1N1B4_30 at 34.454 kb on + strand, within Pf1N1B4_30 at 34.455 kb on - strand at 34.455 kb on - strand, within Pf1N1B4_30 at 34.455 kb on - strand at 34.518 kb on + strand, within Pf1N1B4_30 at 34.556 kb on - strand, within Pf1N1B4_30 at 34.733 kb on - strand at 34.733 kb on - strand, within Pf1N1B4_30 at 34.913 kb on - strand, within Pf1N1B4_30 at 34.913 kb on - strand, within Pf1N1B4_30 at 35.033 kb on - strand, within Pf1N1B4_30 at 35.164 kb on + strand, within Pf1N1B4_30 at 35.165 kb on - strand, within Pf1N1B4_30 at 35.320 kb on - strand, within Pf1N1B4_30 at 35.463 kb on + strand, within Pf1N1B4_30 at 35.600 kb on - strand at 35.610 kb on + strand at 35.611 kb on - strand at 35.611 kb on - strand at 35.662 kb on - strand at 35.699 kb on + strand at 35.812 kb on - strand at 36.245 kb on + strand at 36.245 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.560 kb on - strand, within Pf1N1B4_31 at 36.750 kb on + strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 31,892 - -1.2 31,952 + +0.1 31,952 + -0.2 31,958 + -0.2 31,958 + -0.2 31,959 - +0.8 31,959 - -1.0 31,959 - -0.1 31,959 - -0.2 31,959 - +0.0 32,001 + -2.3 32,002 - -0.7 32,144 + Pf1N1B4_28 0.79 +0.3 32,170 + -1.2 32,171 - +1.6 32,171 - +1.8 32,223 - Pf1N1B4_29 0.18 -1.3 32,223 - Pf1N1B4_29 0.18 -0.2 32,232 - Pf1N1B4_29 0.22 -2.0 32,232 - Pf1N1B4_29 0.22 -2.2 32,232 - Pf1N1B4_29 0.22 +0.8 32,463 + +0.3 32,517 + +3.8 32,526 - +0.8 32,811 + -1.0 32,844 + -0.2 32,845 - +0.6 32,880 + -0.3 32,880 + +0.3 32,880 + -1.2 32,881 - +2.1 32,881 - -0.7 32,881 - -0.3 32,943 + -1.2 32,944 - -1.5 33,271 + +2.8 33,272 - +1.2 33,275 - -0.7 33,275 - Pf1N1B4_30 0.15 +1.5 33,639 - Pf1N1B4_30 0.27 +1.1 33,639 - Pf1N1B4_30 0.27 +0.7 33,655 + Pf1N1B4_30 0.27 -0.5 33,916 - Pf1N1B4_30 0.36 -0.2 34,061 + Pf1N1B4_30 0.40 -0.4 34,083 + Pf1N1B4_30 0.41 +1.3 34,084 - Pf1N1B4_30 0.41 -0.5 34,084 - Pf1N1B4_30 0.41 +1.8 34,110 + Pf1N1B4_30 0.42 -0.2 34,111 - Pf1N1B4_30 0.42 -0.4 34,187 + Pf1N1B4_30 0.45 +1.1 34,188 - -1.2 34,188 - Pf1N1B4_30 0.45 -1.1 34,188 - Pf1N1B4_30 0.45 +0.1 34,190 - Pf1N1B4_30 0.45 +2.3 34,368 + Pf1N1B4_30 0.50 +1.8 34,368 + Pf1N1B4_30 0.50 -0.6 34,369 - Pf1N1B4_30 0.50 +0.4 34,384 - Pf1N1B4_30 0.51 -1.7 34,451 - Pf1N1B4_30 0.53 +0.8 34,451 - Pf1N1B4_30 0.53 +1.4 34,454 + Pf1N1B4_30 0.53 -1.7 34,455 - -1.2 34,455 - Pf1N1B4_30 0.53 -2.0 34,455 - -1.2 34,518 + Pf1N1B4_30 0.55 -1.2 34,556 - Pf1N1B4_30 0.56 -1.5 34,733 - +0.8 34,733 - Pf1N1B4_30 0.62 -1.7 34,913 - Pf1N1B4_30 0.68 +0.0 34,913 - Pf1N1B4_30 0.68 +0.4 35,033 - Pf1N1B4_30 0.72 -2.3 35,164 + Pf1N1B4_30 0.76 -0.2 35,165 - Pf1N1B4_30 0.76 -0.6 35,320 - Pf1N1B4_30 0.81 +0.6 35,463 + Pf1N1B4_30 0.86 -0.4 35,600 - -1.0 35,610 + -2.5 35,611 - +0.8 35,611 - -0.5 35,662 - +0.4 35,699 + -0.2 35,812 - -2.0 36,245 + +0.4 36,245 + Pf1N1B4_31 0.31 +2.1 36,370 + Pf1N1B4_31 0.42 -1.0 36,370 + Pf1N1B4_31 0.42 +1.0 36,479 - Pf1N1B4_31 0.52 -1.5 36,479 - Pf1N1B4_31 0.52 +0.4 36,479 - Pf1N1B4_31 0.52 -1.2 36,479 - Pf1N1B4_31 0.52 +1.1 36,560 - Pf1N1B4_31 0.59 -1.7 36,750 + Pf1N1B4_31 0.76 +1.7 36,751 - Pf1N1B4_31 0.76 +0.7 36,751 - Pf1N1B4_31 0.76 +0.1
Or see this region's nucleotide sequence