Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2713 and Pf1N1B4_2714 are separated by 139 nucleotides Pf1N1B4_2714 and Pf1N1B4_2715 are separated by 27 nucleotides Pf1N1B4_2715 and Pf1N1B4_2716 are separated by 32 nucleotides Pf1N1B4_2716 and Pf1N1B4_2717 are separated by 18 nucleotides Pf1N1B4_2717 and Pf1N1B4_2718 are separated by 18 nucleotides
Pf1N1B4_2713 - FIG00955339: hypothetical protein, at 3,051,182 to 3,051,511
_2713
Pf1N1B4_2714 - FOG: TPR repeat, SEL1 subfamily, at 3,051,651 to 3,052,205
_2714
Pf1N1B4_2715 - hypothetical protein, at 3,052,233 to 3,052,397
_2715
Pf1N1B4_2716 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 3,052,430 to 3,053,713
_2716
Pf1N1B4_2717 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 3,053,732 to 3,054,355
_2717
Pf1N1B4_2718 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 3,054,374 to 3,055,102
_2718
Position (kb)
3052
3053
3054 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3051.565 kb on + strand at 3051.566 kb on - strand at 3051.566 kb on - strand at 3051.566 kb on - strand at 3051.566 kb on - strand at 3051.706 kb on + strand at 3051.706 kb on + strand at 3051.706 kb on + strand at 3051.706 kb on + strand at 3051.706 kb on + strand at 3051.706 kb on + strand at 3051.707 kb on - strand, within Pf1N1B4_2714 at 3051.707 kb on - strand, within Pf1N1B4_2714 at 3051.707 kb on - strand, within Pf1N1B4_2714 at 3051.707 kb on - strand, within Pf1N1B4_2714 at 3051.707 kb on - strand, within Pf1N1B4_2714 at 3051.707 kb on - strand, within Pf1N1B4_2714 at 3051.707 kb on - strand, within Pf1N1B4_2714 at 3051.707 kb on - strand, within Pf1N1B4_2714 at 3051.707 kb on - strand, within Pf1N1B4_2714 at 3051.711 kb on - strand, within Pf1N1B4_2714 at 3052.218 kb on - strand at 3052.274 kb on - strand, within Pf1N1B4_2715 at 3052.274 kb on - strand, within Pf1N1B4_2715 at 3052.274 kb on - strand, within Pf1N1B4_2715 at 3052.274 kb on - strand, within Pf1N1B4_2715 at 3052.274 kb on - strand, within Pf1N1B4_2715 at 3052.274 kb on - strand, within Pf1N1B4_2715 at 3052.290 kb on - strand, within Pf1N1B4_2715 at 3052.327 kb on - strand, within Pf1N1B4_2715 at 3052.327 kb on - strand, within Pf1N1B4_2715 at 3052.327 kb on - strand, within Pf1N1B4_2715 at 3052.327 kb on - strand, within Pf1N1B4_2715 at 3052.327 kb on - strand, within Pf1N1B4_2715 at 3052.327 kb on - strand, within Pf1N1B4_2715 at 3052.327 kb on - strand, within Pf1N1B4_2715 at 3052.327 kb on - strand, within Pf1N1B4_2715 at 3052.329 kb on - strand, within Pf1N1B4_2715 at 3052.329 kb on - strand, within Pf1N1B4_2715 at 3052.329 kb on - strand, within Pf1N1B4_2715 at 3052.329 kb on - strand, within Pf1N1B4_2715 at 3052.329 kb on - strand, within Pf1N1B4_2715 at 3052.329 kb on - strand, within Pf1N1B4_2715 at 3052.329 kb on - strand, within Pf1N1B4_2715 at 3052.329 kb on - strand, within Pf1N1B4_2715 at 3052.351 kb on - strand, within Pf1N1B4_2715 at 3052.351 kb on - strand, within Pf1N1B4_2715 at 3052.385 kb on - strand at 3052.432 kb on - strand at 3052.432 kb on - strand at 3053.989 kb on - strand, within Pf1N1B4_2717 at 3053.989 kb on - strand, within Pf1N1B4_2717 at 3053.989 kb on - strand, within Pf1N1B4_2717 at 3053.989 kb on - strand, within Pf1N1B4_2717 at 3054.315 kb on - strand at 3054.347 kb on + strand at 3054.348 kb on - strand at 3054.348 kb on - strand at 3054.348 kb on - strand at 3054.348 kb on - strand at 3054.460 kb on - strand, within Pf1N1B4_2718 at 3054.460 kb on - strand at 3054.460 kb on - strand, within Pf1N1B4_2718 at 3054.460 kb on - strand, within Pf1N1B4_2718 at 3054.552 kb on + strand, within Pf1N1B4_2718 at 3054.552 kb on + strand, within Pf1N1B4_2718 at 3054.552 kb on + strand, within Pf1N1B4_2718 at 3054.553 kb on - strand, within Pf1N1B4_2718 at 3054.606 kb on + strand, within Pf1N1B4_2718 at 3054.606 kb on + strand, within Pf1N1B4_2718 at 3054.606 kb on + strand, within Pf1N1B4_2718 at 3054.607 kb on - strand, within Pf1N1B4_2718 at 3054.607 kb on - strand, within Pf1N1B4_2718 at 3054.607 kb on - strand, within Pf1N1B4_2718 at 3054.607 kb on - strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.620 kb on + strand at 3054.620 kb on + strand, within Pf1N1B4_2718 at 3054.621 kb on - strand, within Pf1N1B4_2718
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 3,051,565 + +2.2 3,051,566 - -1.7 3,051,566 - +1.5 3,051,566 - +0.7 3,051,566 - -0.7 3,051,706 + -2.0 3,051,706 + -0.7 3,051,706 + -1.7 3,051,706 + -0.4 3,051,706 + +0.5 3,051,706 + -1.2 3,051,707 - Pf1N1B4_2714 0.10 -2.3 3,051,707 - Pf1N1B4_2714 0.10 +0.3 3,051,707 - Pf1N1B4_2714 0.10 -0.5 3,051,707 - Pf1N1B4_2714 0.10 -0.7 3,051,707 - Pf1N1B4_2714 0.10 -0.3 3,051,707 - Pf1N1B4_2714 0.10 -0.8 3,051,707 - Pf1N1B4_2714 0.10 +0.0 3,051,707 - Pf1N1B4_2714 0.10 -0.7 3,051,707 - Pf1N1B4_2714 0.10 +0.9 3,051,711 - Pf1N1B4_2714 0.11 -0.4 3,052,218 - -0.2 3,052,274 - Pf1N1B4_2715 0.25 -1.5 3,052,274 - Pf1N1B4_2715 0.25 +0.4 3,052,274 - Pf1N1B4_2715 0.25 +0.4 3,052,274 - Pf1N1B4_2715 0.25 -0.2 3,052,274 - Pf1N1B4_2715 0.25 +0.2 3,052,274 - Pf1N1B4_2715 0.25 +0.6 3,052,290 - Pf1N1B4_2715 0.35 -1.3 3,052,327 - Pf1N1B4_2715 0.57 -0.3 3,052,327 - Pf1N1B4_2715 0.57 +0.1 3,052,327 - Pf1N1B4_2715 0.57 -1.7 3,052,327 - Pf1N1B4_2715 0.57 +0.5 3,052,327 - Pf1N1B4_2715 0.57 +0.4 3,052,327 - Pf1N1B4_2715 0.57 +1.2 3,052,327 - Pf1N1B4_2715 0.57 -1.4 3,052,327 - Pf1N1B4_2715 0.57 -1.7 3,052,329 - Pf1N1B4_2715 0.58 +1.5 3,052,329 - Pf1N1B4_2715 0.58 -0.6 3,052,329 - Pf1N1B4_2715 0.58 +0.6 3,052,329 - Pf1N1B4_2715 0.58 +1.5 3,052,329 - Pf1N1B4_2715 0.58 -0.4 3,052,329 - Pf1N1B4_2715 0.58 -0.6 3,052,329 - Pf1N1B4_2715 0.58 +0.2 3,052,329 - Pf1N1B4_2715 0.58 +0.5 3,052,351 - Pf1N1B4_2715 0.72 -0.4 3,052,351 - Pf1N1B4_2715 0.72 +0.6 3,052,385 - -1.2 3,052,432 - -0.8 3,052,432 - +1.5 3,053,989 - Pf1N1B4_2717 0.41 +2.7 3,053,989 - Pf1N1B4_2717 0.41 +1.2 3,053,989 - Pf1N1B4_2717 0.41 -0.2 3,053,989 - Pf1N1B4_2717 0.41 +0.7 3,054,315 - -0.2 3,054,347 + +3.0 3,054,348 - +0.2 3,054,348 - +0.3 3,054,348 - -0.1 3,054,348 - -1.8 3,054,460 - Pf1N1B4_2718 0.12 -0.3 3,054,460 - +0.2 3,054,460 - Pf1N1B4_2718 0.12 +0.3 3,054,460 - Pf1N1B4_2718 0.12 -0.8 3,054,552 + Pf1N1B4_2718 0.24 -0.1 3,054,552 + Pf1N1B4_2718 0.24 -2.0 3,054,552 + Pf1N1B4_2718 0.24 +0.9 3,054,553 - Pf1N1B4_2718 0.25 -0.1 3,054,606 + Pf1N1B4_2718 0.32 -0.4 3,054,606 + Pf1N1B4_2718 0.32 +0.8 3,054,606 + Pf1N1B4_2718 0.32 -0.2 3,054,607 - Pf1N1B4_2718 0.32 +1.3 3,054,607 - Pf1N1B4_2718 0.32 -0.2 3,054,607 - Pf1N1B4_2718 0.32 -0.2 3,054,607 - Pf1N1B4_2718 0.32 -0.8 3,054,620 + Pf1N1B4_2718 0.34 -0.2 3,054,620 + Pf1N1B4_2718 0.34 -1.2 3,054,620 + Pf1N1B4_2718 0.34 -0.7 3,054,620 + Pf1N1B4_2718 0.34 +1.0 3,054,620 + Pf1N1B4_2718 0.34 -0.1 3,054,620 + Pf1N1B4_2718 0.34 +0.2 3,054,620 + +0.2 3,054,620 + Pf1N1B4_2718 0.34 +0.7 3,054,621 - Pf1N1B4_2718 0.34 +0.3
Or see this region's nucleotide sequence