Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2232 and Pf1N1B4_2233 are separated by 210 nucleotides Pf1N1B4_2233 and Pf1N1B4_2234 are separated by 404 nucleotides
Pf1N1B4_2232 - Ferric iron ABC transporter, iron-binding protein, at 2,544,096 to 2,545,100
_2232
Pf1N1B4_2233 - Ferric iron ABC transporter, permease protein, at 2,545,311 to 2,546,894
_2233
Pf1N1B4_2234 - Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10), at 2,547,299 to 2,548,132
_2234
Position (kb)
2545
2546
2547 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2544.429 kb on + strand, within Pf1N1B4_2232 at 2544.486 kb on + strand, within Pf1N1B4_2232 at 2544.487 kb on - strand, within Pf1N1B4_2232 at 2544.525 kb on + strand, within Pf1N1B4_2232 at 2544.525 kb on + strand, within Pf1N1B4_2232 at 2544.526 kb on - strand, within Pf1N1B4_2232 at 2544.526 kb on - strand, within Pf1N1B4_2232 at 2544.588 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.756 kb on + strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.757 kb on - strand, within Pf1N1B4_2232 at 2544.759 kb on + strand at 2544.997 kb on + strand, within Pf1N1B4_2232 at 2545.031 kb on - strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.112 kb on + strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.113 kb on - strand at 2545.262 kb on + strand at 2545.575 kb on + strand, within Pf1N1B4_2233 at 2545.575 kb on + strand, within Pf1N1B4_2233 at 2545.575 kb on + strand, within Pf1N1B4_2233 at 2545.575 kb on + strand at 2545.575 kb on + strand at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.576 kb on - strand, within Pf1N1B4_2233 at 2545.731 kb on + strand, within Pf1N1B4_2233 at 2545.732 kb on - strand, within Pf1N1B4_2233 at 2545.732 kb on - strand, within Pf1N1B4_2233 at 2545.732 kb on - strand, within Pf1N1B4_2233 at 2545.928 kb on + strand, within Pf1N1B4_2233 at 2545.947 kb on + strand at 2545.948 kb on - strand, within Pf1N1B4_2233 at 2546.032 kb on - strand, within Pf1N1B4_2233 at 2546.032 kb on - strand, within Pf1N1B4_2233 at 2546.097 kb on + strand, within Pf1N1B4_2233 at 2546.097 kb on + strand, within Pf1N1B4_2233 at 2546.149 kb on + strand, within Pf1N1B4_2233 at 2546.149 kb on + strand, within Pf1N1B4_2233 at 2546.150 kb on - strand, within Pf1N1B4_2233 at 2546.150 kb on - strand, within Pf1N1B4_2233 at 2546.232 kb on + strand, within Pf1N1B4_2233 at 2546.232 kb on + strand, within Pf1N1B4_2233 at 2546.259 kb on + strand, within Pf1N1B4_2233 at 2546.260 kb on - strand, within Pf1N1B4_2233 at 2546.427 kb on + strand, within Pf1N1B4_2233 at 2546.428 kb on - strand at 2546.523 kb on + strand, within Pf1N1B4_2233 at 2546.524 kb on - strand, within Pf1N1B4_2233 at 2546.609 kb on + strand, within Pf1N1B4_2233 at 2546.610 kb on - strand, within Pf1N1B4_2233 at 2546.649 kb on + strand, within Pf1N1B4_2233 at 2546.649 kb on + strand, within Pf1N1B4_2233 at 2546.649 kb on + strand, within Pf1N1B4_2233 at 2546.650 kb on - strand, within Pf1N1B4_2233 at 2546.650 kb on - strand, within Pf1N1B4_2233 at 2546.892 kb on + strand at 2546.892 kb on + strand at 2546.905 kb on + strand at 2546.906 kb on - strand at 2546.906 kb on - strand at 2546.918 kb on + strand at 2546.918 kb on + strand at 2546.918 kb on + strand at 2546.919 kb on - strand at 2547.063 kb on - strand at 2547.073 kb on - strand at 2547.073 kb on - strand at 2547.196 kb on + strand at 2547.252 kb on - strand at 2547.255 kb on + strand at 2547.256 kb on - strand at 2547.303 kb on - strand at 2547.303 kb on - strand at 2547.354 kb on - strand at 2547.563 kb on + strand, within Pf1N1B4_2234 at 2547.564 kb on - strand, within Pf1N1B4_2234 at 2547.691 kb on + strand, within Pf1N1B4_2234 at 2547.692 kb on - strand, within Pf1N1B4_2234 at 2547.879 kb on + strand, within Pf1N1B4_2234 at 2547.879 kb on + strand, within Pf1N1B4_2234
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 2,544,429 + Pf1N1B4_2232 0.33 +0.4 2,544,486 + Pf1N1B4_2232 0.39 -1.1 2,544,487 - Pf1N1B4_2232 0.39 -0.5 2,544,525 + Pf1N1B4_2232 0.43 +0.9 2,544,525 + Pf1N1B4_2232 0.43 -1.3 2,544,526 - Pf1N1B4_2232 0.43 +1.8 2,544,526 - Pf1N1B4_2232 0.43 -2.1 2,544,588 + Pf1N1B4_2232 0.49 -0.7 2,544,756 + Pf1N1B4_2232 0.66 +0.4 2,544,756 + Pf1N1B4_2232 0.66 -1.3 2,544,756 + Pf1N1B4_2232 0.66 -2.1 2,544,756 + Pf1N1B4_2232 0.66 +0.3 2,544,756 + Pf1N1B4_2232 0.66 +0.4 2,544,757 - Pf1N1B4_2232 0.66 +0.0 2,544,757 - Pf1N1B4_2232 0.66 +0.4 2,544,757 - Pf1N1B4_2232 0.66 -1.1 2,544,757 - Pf1N1B4_2232 0.66 +1.6 2,544,757 - Pf1N1B4_2232 0.66 -2.3 2,544,759 + +0.3 2,544,997 + Pf1N1B4_2232 0.90 -1.7 2,545,031 - -0.7 2,545,112 + -0.3 2,545,112 + -0.3 2,545,112 + +0.6 2,545,112 + -0.1 2,545,112 + -0.7 2,545,112 + +0.6 2,545,112 + -1.1 2,545,112 + -0.3 2,545,112 + +2.8 2,545,113 - -1.9 2,545,113 - +1.3 2,545,113 - -1.9 2,545,113 - -2.2 2,545,113 - -0.8 2,545,113 - -0.1 2,545,113 - -0.2 2,545,262 + -0.3 2,545,575 + Pf1N1B4_2233 0.17 +0.8 2,545,575 + Pf1N1B4_2233 0.17 -1.0 2,545,575 + Pf1N1B4_2233 0.17 +0.7 2,545,575 + +2.3 2,545,575 + +0.3 2,545,576 - Pf1N1B4_2233 0.17 +0.7 2,545,576 - Pf1N1B4_2233 0.17 +0.5 2,545,576 - Pf1N1B4_2233 0.17 +1.0 2,545,576 - Pf1N1B4_2233 0.17 -0.3 2,545,576 - Pf1N1B4_2233 0.17 -0.3 2,545,576 - Pf1N1B4_2233 0.17 -2.2 2,545,576 - Pf1N1B4_2233 0.17 -2.6 2,545,576 - Pf1N1B4_2233 0.17 -1.2 2,545,576 - Pf1N1B4_2233 0.17 -2.3 2,545,731 + Pf1N1B4_2233 0.27 -1.3 2,545,732 - Pf1N1B4_2233 0.27 -1.1 2,545,732 - Pf1N1B4_2233 0.27 -1.4 2,545,732 - Pf1N1B4_2233 0.27 -1.5 2,545,928 + Pf1N1B4_2233 0.39 +1.3 2,545,947 + +1.3 2,545,948 - Pf1N1B4_2233 0.40 -3.0 2,546,032 - Pf1N1B4_2233 0.46 -2.1 2,546,032 - Pf1N1B4_2233 0.46 -1.1 2,546,097 + Pf1N1B4_2233 0.50 -0.5 2,546,097 + Pf1N1B4_2233 0.50 -0.2 2,546,149 + Pf1N1B4_2233 0.53 -0.2 2,546,149 + Pf1N1B4_2233 0.53 -0.5 2,546,150 - Pf1N1B4_2233 0.53 +0.4 2,546,150 - Pf1N1B4_2233 0.53 -1.3 2,546,232 + Pf1N1B4_2233 0.58 -1.6 2,546,232 + Pf1N1B4_2233 0.58 -1.7 2,546,259 + Pf1N1B4_2233 0.60 -1.3 2,546,260 - Pf1N1B4_2233 0.60 -2.4 2,546,427 + Pf1N1B4_2233 0.70 -0.4 2,546,428 - +1.3 2,546,523 + Pf1N1B4_2233 0.77 -0.0 2,546,524 - Pf1N1B4_2233 0.77 +2.8 2,546,609 + Pf1N1B4_2233 0.82 -0.3 2,546,610 - Pf1N1B4_2233 0.82 -1.4 2,546,649 + Pf1N1B4_2233 0.84 +0.0 2,546,649 + Pf1N1B4_2233 0.84 +1.3 2,546,649 + Pf1N1B4_2233 0.84 -0.7 2,546,650 - Pf1N1B4_2233 0.85 -2.1 2,546,650 - Pf1N1B4_2233 0.85 -1.3 2,546,892 + +0.5 2,546,892 + -0.6 2,546,905 + +1.3 2,546,906 - +0.3 2,546,906 - -0.7 2,546,918 + -2.2 2,546,918 + -1.7 2,546,918 + +0.3 2,546,919 - -2.3 2,547,063 - -1.5 2,547,073 - -0.6 2,547,073 - +0.9 2,547,196 + -1.2 2,547,252 - -2.1 2,547,255 + +0.9 2,547,256 - +0.1 2,547,303 - -0.5 2,547,303 - -1.1 2,547,354 - +0.1 2,547,563 + Pf1N1B4_2234 0.32 +1.7 2,547,564 - Pf1N1B4_2234 0.32 -1.3 2,547,691 + Pf1N1B4_2234 0.47 -0.1 2,547,692 - Pf1N1B4_2234 0.47 +0.4 2,547,879 + Pf1N1B4_2234 0.70 -0.0 2,547,879 + Pf1N1B4_2234 0.70 -0.3
Or see this region's nucleotide sequence