Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2185 and Pf1N1B4_2186 overlap by 4 nucleotides Pf1N1B4_2186 and Pf1N1B4_2187 are separated by 16 nucleotides Pf1N1B4_2187 and Pf1N1B4_2188 overlap by 4 nucleotides
Pf1N1B4_2185 - Porphobilinogen deaminase (EC 2.5.1.61), at 2,497,352 to 2,498,293
_2185
Pf1N1B4_2186 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,498,290 to 2,499,057
_2186
Pf1N1B4_2187 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,499,074 to 2,500,228
_2187
Pf1N1B4_2188 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 2,500,225 to 2,501,463
_2188
Position (kb)
2499
2500
2501 Strain fitness (log2 ratio)
-1
0
1 at 2498.270 kb on + strand at 2498.270 kb on + strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2499.071 kb on + strand at 2500.881 kb on - strand, within Pf1N1B4_2188 at 2501.047 kb on + strand, within Pf1N1B4_2188 at 2501.122 kb on + strand, within Pf1N1B4_2188 at 2501.122 kb on + strand, within Pf1N1B4_2188
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 2,498,270 + +0.5 2,498,270 + -0.4 2,498,271 - -1.1 2,498,271 - +0.9 2,498,271 - +0.1 2,498,271 - +0.7 2,499,071 + +0.3 2,500,881 - Pf1N1B4_2188 0.53 -0.3 2,501,047 + Pf1N1B4_2188 0.66 +0.3 2,501,122 + Pf1N1B4_2188 0.72 +0.0 2,501,122 + Pf1N1B4_2188 0.72 +0.4
Or see this region's nucleotide sequence