Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2187

Experiment: Rifampicin 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2185 and Pf1N1B4_2186 overlap by 4 nucleotidesPf1N1B4_2186 and Pf1N1B4_2187 are separated by 16 nucleotidesPf1N1B4_2187 and Pf1N1B4_2188 overlap by 4 nucleotides Pf1N1B4_2185 - Porphobilinogen deaminase (EC 2.5.1.61), at 2,497,352 to 2,498,293 _2185 Pf1N1B4_2186 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,498,290 to 2,499,057 _2186 Pf1N1B4_2187 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,499,074 to 2,500,228 _2187 Pf1N1B4_2188 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 2,500,225 to 2,501,463 _2188 Position (kb) 2499 2500 2501Strain fitness (log2 ratio) -1 0 1at 2498.270 kb on + strandat 2498.270 kb on + strandat 2498.271 kb on - strandat 2498.271 kb on - strandat 2498.271 kb on - strandat 2498.271 kb on - strandat 2499.071 kb on + strandat 2500.881 kb on - strand, within Pf1N1B4_2188at 2501.047 kb on + strand, within Pf1N1B4_2188at 2501.122 kb on + strand, within Pf1N1B4_2188at 2501.122 kb on + strand, within Pf1N1B4_2188

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Per-strain Table

Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml
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2,498,270 + +0.5
2,498,270 + -0.4
2,498,271 - -1.1
2,498,271 - +0.9
2,498,271 - +0.1
2,498,271 - +0.7
2,499,071 + +0.3
2,500,881 - Pf1N1B4_2188 0.53 -0.3
2,501,047 + Pf1N1B4_2188 0.66 +0.3
2,501,122 + Pf1N1B4_2188 0.72 +0.0
2,501,122 + Pf1N1B4_2188 0.72 +0.4

Or see this region's nucleotide sequence