Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2184 and Pf1N1B4_2185 are separated by 126 nucleotides Pf1N1B4_2185 and Pf1N1B4_2186 overlap by 4 nucleotides Pf1N1B4_2186 and Pf1N1B4_2187 are separated by 16 nucleotides
Pf1N1B4_2184 - Autolysis response regulater LytR, at 2,496,479 to 2,497,225
_2184
Pf1N1B4_2185 - Porphobilinogen deaminase (EC 2.5.1.61), at 2,497,352 to 2,498,293
_2185
Pf1N1B4_2186 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,498,290 to 2,499,057
_2186
Pf1N1B4_2187 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 2,499,074 to 2,500,228
_2187
Position (kb)
2497
2498
2499 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2496.392 kb on + strand at 2496.393 kb on - strand at 2496.436 kb on + strand at 2496.478 kb on + strand at 2496.479 kb on - strand at 2496.479 kb on - strand at 2496.479 kb on - strand at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.554 kb on + strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.555 kb on - strand, within Pf1N1B4_2184 at 2496.753 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.804 kb on + strand, within Pf1N1B4_2184 at 2496.805 kb on - strand, within Pf1N1B4_2184 at 2496.805 kb on - strand, within Pf1N1B4_2184 at 2496.816 kb on + strand, within Pf1N1B4_2184 at 2496.957 kb on - strand, within Pf1N1B4_2184 at 2496.957 kb on - strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.977 kb on + strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2496.978 kb on - strand, within Pf1N1B4_2184 at 2497.243 kb on + strand at 2497.243 kb on + strand at 2497.243 kb on + strand at 2497.244 kb on - strand at 2497.244 kb on - strand at 2497.244 kb on - strand at 2497.244 kb on - strand at 2497.286 kb on + strand at 2497.286 kb on + strand at 2497.286 kb on + strand at 2497.287 kb on - strand at 2497.287 kb on - strand at 2497.316 kb on + strand at 2497.316 kb on + strand at 2497.317 kb on - strand at 2497.319 kb on + strand at 2497.319 kb on + strand at 2497.319 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2497.332 kb on + strand at 2498.270 kb on + strand at 2498.270 kb on + strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2498.271 kb on - strand at 2499.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 2,496,392 + -0.1 2,496,393 - +2.3 2,496,436 + -0.9 2,496,478 + -1.1 2,496,479 - +1.7 2,496,479 - -2.4 2,496,479 - +1.0 2,496,554 + Pf1N1B4_2184 0.10 +0.1 2,496,554 + Pf1N1B4_2184 0.10 -0.1 2,496,554 + Pf1N1B4_2184 0.10 -0.1 2,496,554 + Pf1N1B4_2184 0.10 -0.5 2,496,554 + Pf1N1B4_2184 0.10 +1.9 2,496,555 - Pf1N1B4_2184 0.10 +2.5 2,496,555 - Pf1N1B4_2184 0.10 +0.8 2,496,555 - Pf1N1B4_2184 0.10 -0.5 2,496,555 - Pf1N1B4_2184 0.10 -0.3 2,496,753 + Pf1N1B4_2184 0.37 -0.1 2,496,804 + Pf1N1B4_2184 0.44 +1.1 2,496,804 + Pf1N1B4_2184 0.44 -0.2 2,496,804 + Pf1N1B4_2184 0.44 -0.3 2,496,804 + Pf1N1B4_2184 0.44 +1.0 2,496,805 - Pf1N1B4_2184 0.44 -0.4 2,496,805 - Pf1N1B4_2184 0.44 +1.0 2,496,816 + Pf1N1B4_2184 0.45 +0.9 2,496,957 - Pf1N1B4_2184 0.64 +1.4 2,496,957 - Pf1N1B4_2184 0.64 +0.3 2,496,977 + Pf1N1B4_2184 0.67 +1.3 2,496,977 + Pf1N1B4_2184 0.67 +1.1 2,496,977 + Pf1N1B4_2184 0.67 +0.3 2,496,977 + Pf1N1B4_2184 0.67 -2.3 2,496,978 - Pf1N1B4_2184 0.67 +0.5 2,496,978 - Pf1N1B4_2184 0.67 -1.5 2,496,978 - Pf1N1B4_2184 0.67 +0.3 2,496,978 - Pf1N1B4_2184 0.67 -0.7 2,496,978 - Pf1N1B4_2184 0.67 +1.7 2,496,978 - Pf1N1B4_2184 0.67 -2.4 2,496,978 - Pf1N1B4_2184 0.67 +0.4 2,496,978 - Pf1N1B4_2184 0.67 -1.5 2,496,978 - Pf1N1B4_2184 0.67 -0.9 2,497,243 + +0.3 2,497,243 + -0.6 2,497,243 + -1.2 2,497,244 - -1.2 2,497,244 - -2.2 2,497,244 - +0.7 2,497,244 - +0.5 2,497,286 + -0.5 2,497,286 + -0.7 2,497,286 + +0.9 2,497,287 - +0.9 2,497,287 - -0.3 2,497,316 + +0.3 2,497,316 + +0.7 2,497,317 - +1.0 2,497,319 + -0.6 2,497,319 + -0.7 2,497,319 + +0.0 2,497,332 + +0.5 2,497,332 + -0.3 2,497,332 + +0.7 2,497,332 + -0.9 2,497,332 + -1.7 2,497,332 + +0.3 2,498,270 + +0.5 2,498,270 + -0.4 2,498,271 - -1.1 2,498,271 - +0.9 2,498,271 - +0.1 2,498,271 - +0.7 2,499,071 + +0.3
Or see this region's nucleotide sequence