Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1906 and Pf1N1B4_1907 are separated by 81 nucleotides Pf1N1B4_1907 and Pf1N1B4_1908 are separated by 173 nucleotides
Pf1N1B4_1906 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 2,180,656 to 2,181,477
_1906
Pf1N1B4_1907 - Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3), at 2,181,559 to 2,182,473
_1907
Pf1N1B4_1908 - Quinone oxidoreductase (EC 1.6.5.5), at 2,182,647 to 2,183,624
_1908
Position (kb)
2181
2182
2183 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2180.564 kb on + strand at 2180.564 kb on + strand at 2180.565 kb on - strand at 2180.649 kb on - strand at 2180.649 kb on - strand at 2180.726 kb on + strand at 2180.727 kb on - strand at 2180.831 kb on + strand, within Pf1N1B4_1906 at 2180.831 kb on + strand, within Pf1N1B4_1906 at 2180.832 kb on - strand, within Pf1N1B4_1906 at 2180.832 kb on - strand, within Pf1N1B4_1906 at 2180.832 kb on - strand, within Pf1N1B4_1906 at 2180.844 kb on - strand, within Pf1N1B4_1906 at 2180.844 kb on - strand, within Pf1N1B4_1906 at 2180.890 kb on + strand, within Pf1N1B4_1906 at 2180.890 kb on + strand, within Pf1N1B4_1906 at 2180.891 kb on - strand, within Pf1N1B4_1906 at 2181.121 kb on - strand, within Pf1N1B4_1906 at 2181.380 kb on + strand, within Pf1N1B4_1906 at 2181.381 kb on - strand, within Pf1N1B4_1906 at 2181.381 kb on - strand, within Pf1N1B4_1906 at 2181.381 kb on - strand, within Pf1N1B4_1906 at 2181.460 kb on + strand at 2181.461 kb on - strand at 2181.468 kb on - strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.480 kb on - strand at 2181.495 kb on - strand at 2181.495 kb on - strand at 2181.495 kb on - strand at 2181.495 kb on - strand at 2182.499 kb on + strand at 2182.499 kb on + strand at 2182.499 kb on + strand at 2182.500 kb on - strand at 2182.513 kb on - strand at 2182.538 kb on - strand at 2182.538 kb on - strand at 2182.596 kb on + strand at 2182.597 kb on - strand at 2182.612 kb on - strand at 2182.619 kb on + strand at 2182.619 kb on + strand at 2182.619 kb on + strand at 2182.620 kb on - strand at 2182.620 kb on - strand at 2182.690 kb on + strand at 2182.691 kb on - strand at 2182.707 kb on + strand at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.782 kb on + strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.783 kb on - strand, within Pf1N1B4_1908 at 2182.786 kb on - strand, within Pf1N1B4_1908 at 2182.800 kb on + strand, within Pf1N1B4_1908 at 2182.801 kb on - strand, within Pf1N1B4_1908 at 2182.929 kb on + strand, within Pf1N1B4_1908 at 2182.929 kb on + strand, within Pf1N1B4_1908 at 2182.929 kb on + strand, within Pf1N1B4_1908 at 2182.929 kb on + strand, within Pf1N1B4_1908 at 2182.930 kb on - strand, within Pf1N1B4_1908 at 2182.931 kb on + strand, within Pf1N1B4_1908 at 2182.931 kb on + strand, within Pf1N1B4_1908 at 2182.932 kb on - strand, within Pf1N1B4_1908 at 2182.932 kb on - strand, within Pf1N1B4_1908 at 2182.945 kb on + strand, within Pf1N1B4_1908 at 2182.945 kb on + strand, within Pf1N1B4_1908 at 2182.945 kb on + strand, within Pf1N1B4_1908 at 2182.945 kb on + strand, within Pf1N1B4_1908 at 2182.946 kb on - strand, within Pf1N1B4_1908 at 2182.946 kb on - strand, within Pf1N1B4_1908 at 2182.966 kb on + strand, within Pf1N1B4_1908 at 2182.966 kb on + strand, within Pf1N1B4_1908 at 2182.966 kb on + strand, within Pf1N1B4_1908 at 2182.966 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.052 kb on + strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.053 kb on - strand, within Pf1N1B4_1908 at 2183.220 kb on + strand at 2183.220 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.286 kb on + strand, within Pf1N1B4_1908 at 2183.287 kb on - strand, within Pf1N1B4_1908 at 2183.287 kb on - strand, within Pf1N1B4_1908 at 2183.287 kb on - strand, within Pf1N1B4_1908 at 2183.287 kb on - strand, within Pf1N1B4_1908 at 2183.380 kb on + strand, within Pf1N1B4_1908 at 2183.381 kb on - strand, within Pf1N1B4_1908 at 2183.424 kb on + strand, within Pf1N1B4_1908 at 2183.424 kb on + strand, within Pf1N1B4_1908 at 2183.424 kb on + strand, within Pf1N1B4_1908 at 2183.424 kb on + strand, within Pf1N1B4_1908 at 2183.424 kb on + strand at 2183.425 kb on - strand, within Pf1N1B4_1908 at 2183.451 kb on + strand, within Pf1N1B4_1908 at 2183.451 kb on + strand, within Pf1N1B4_1908
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 2,180,564 + +2.9 2,180,564 + -0.3 2,180,565 - +0.3 2,180,649 - -0.5 2,180,649 - -1.0 2,180,726 + -1.3 2,180,727 - +1.3 2,180,831 + Pf1N1B4_1906 0.21 -0.3 2,180,831 + Pf1N1B4_1906 0.21 -2.6 2,180,832 - Pf1N1B4_1906 0.21 -1.5 2,180,832 - Pf1N1B4_1906 0.21 -1.0 2,180,832 - Pf1N1B4_1906 0.21 +2.7 2,180,844 - Pf1N1B4_1906 0.23 -2.1 2,180,844 - Pf1N1B4_1906 0.23 -1.5 2,180,890 + Pf1N1B4_1906 0.28 -0.7 2,180,890 + Pf1N1B4_1906 0.28 -1.9 2,180,891 - Pf1N1B4_1906 0.29 -3.3 2,181,121 - Pf1N1B4_1906 0.57 -1.9 2,181,380 + Pf1N1B4_1906 0.88 +1.3 2,181,381 - Pf1N1B4_1906 0.88 -1.0 2,181,381 - Pf1N1B4_1906 0.88 -1.3 2,181,381 - Pf1N1B4_1906 0.88 -0.7 2,181,460 + -0.3 2,181,461 - +0.3 2,181,468 - -2.8 2,181,479 + -0.7 2,181,479 + -1.0 2,181,479 + -1.3 2,181,479 + +1.3 2,181,480 - -0.3 2,181,480 - +1.9 2,181,480 - +1.1 2,181,480 - -0.5 2,181,480 - +1.3 2,181,480 - +1.3 2,181,480 - -2.5 2,181,480 - +1.3 2,181,480 - -1.3 2,181,480 - +0.2 2,181,480 - +1.1 2,181,480 - -0.0 2,181,495 - -1.3 2,181,495 - -0.3 2,181,495 - +0.3 2,181,495 - -1.3 2,182,499 + -0.4 2,182,499 + -1.5 2,182,499 + -1.6 2,182,500 - -0.3 2,182,513 - +0.3 2,182,538 - -0.8 2,182,538 - -1.1 2,182,596 + -0.4 2,182,597 - -0.4 2,182,612 - +1.3 2,182,619 + +0.3 2,182,619 + +0.3 2,182,619 + +1.0 2,182,620 - -0.9 2,182,620 - +0.5 2,182,690 + -0.3 2,182,691 - +1.2 2,182,707 + -1.1 2,182,782 + Pf1N1B4_1908 0.14 +0.7 2,182,782 + Pf1N1B4_1908 0.14 +0.5 2,182,782 + Pf1N1B4_1908 0.14 -0.0 2,182,782 + Pf1N1B4_1908 0.14 -1.9 2,182,782 + Pf1N1B4_1908 0.14 -0.7 2,182,782 + -0.7 2,182,782 + Pf1N1B4_1908 0.14 -2.6 2,182,782 + Pf1N1B4_1908 0.14 -2.4 2,182,782 + Pf1N1B4_1908 0.14 -1.3 2,182,782 + Pf1N1B4_1908 0.14 +0.3 2,182,782 + Pf1N1B4_1908 0.14 +0.1 2,182,782 + Pf1N1B4_1908 0.14 +0.8 2,182,782 + Pf1N1B4_1908 0.14 -1.1 2,182,782 + Pf1N1B4_1908 0.14 +1.3 2,182,782 + Pf1N1B4_1908 0.14 -0.4 2,182,782 + Pf1N1B4_1908 0.14 -1.3 2,182,783 - Pf1N1B4_1908 0.14 +0.3 2,182,783 - Pf1N1B4_1908 0.14 -0.4 2,182,783 - Pf1N1B4_1908 0.14 -0.6 2,182,783 - Pf1N1B4_1908 0.14 +0.1 2,182,783 - Pf1N1B4_1908 0.14 +0.8 2,182,783 - Pf1N1B4_1908 0.14 -1.5 2,182,783 - Pf1N1B4_1908 0.14 -0.7 2,182,786 - Pf1N1B4_1908 0.14 -0.2 2,182,800 + Pf1N1B4_1908 0.16 +0.6 2,182,801 - Pf1N1B4_1908 0.16 +0.8 2,182,929 + Pf1N1B4_1908 0.29 -0.5 2,182,929 + Pf1N1B4_1908 0.29 +1.0 2,182,929 + Pf1N1B4_1908 0.29 -3.0 2,182,929 + Pf1N1B4_1908 0.29 -0.8 2,182,930 - Pf1N1B4_1908 0.29 -1.9 2,182,931 + Pf1N1B4_1908 0.29 +0.9 2,182,931 + Pf1N1B4_1908 0.29 -0.7 2,182,932 - Pf1N1B4_1908 0.29 +0.7 2,182,932 - Pf1N1B4_1908 0.29 -1.3 2,182,945 + Pf1N1B4_1908 0.30 +0.2 2,182,945 + Pf1N1B4_1908 0.30 +0.9 2,182,945 + Pf1N1B4_1908 0.30 +2.3 2,182,945 + Pf1N1B4_1908 0.30 -1.0 2,182,946 - Pf1N1B4_1908 0.31 +1.8 2,182,946 - Pf1N1B4_1908 0.31 -0.3 2,182,966 + Pf1N1B4_1908 0.33 -0.5 2,182,966 + Pf1N1B4_1908 0.33 -0.3 2,182,966 + Pf1N1B4_1908 0.33 -0.2 2,182,966 + Pf1N1B4_1908 0.33 +0.3 2,183,052 + Pf1N1B4_1908 0.41 -3.2 2,183,052 + Pf1N1B4_1908 0.41 +0.3 2,183,052 + Pf1N1B4_1908 0.41 -0.7 2,183,052 + Pf1N1B4_1908 0.41 -0.7 2,183,052 + Pf1N1B4_1908 0.41 -0.3 2,183,052 + Pf1N1B4_1908 0.41 +1.6 2,183,052 + Pf1N1B4_1908 0.41 +0.7 2,183,052 + Pf1N1B4_1908 0.41 +0.5 2,183,052 + Pf1N1B4_1908 0.41 -0.4 2,183,052 + Pf1N1B4_1908 0.41 +0.9 2,183,053 - Pf1N1B4_1908 0.42 +0.1 2,183,053 - Pf1N1B4_1908 0.42 +0.9 2,183,053 - Pf1N1B4_1908 0.42 +1.3 2,183,053 - Pf1N1B4_1908 0.42 +1.3 2,183,053 - Pf1N1B4_1908 0.42 -2.4 2,183,220 + +1.3 2,183,220 + Pf1N1B4_1908 0.59 +0.1 2,183,286 + Pf1N1B4_1908 0.65 +0.2 2,183,286 + Pf1N1B4_1908 0.65 -2.4 2,183,286 + Pf1N1B4_1908 0.65 +1.3 2,183,286 + Pf1N1B4_1908 0.65 -1.0 2,183,286 + Pf1N1B4_1908 0.65 -0.5 2,183,287 - Pf1N1B4_1908 0.65 -0.0 2,183,287 - Pf1N1B4_1908 0.65 +0.5 2,183,287 - Pf1N1B4_1908 0.65 -0.0 2,183,287 - Pf1N1B4_1908 0.65 -1.7 2,183,380 + Pf1N1B4_1908 0.75 +0.9 2,183,381 - Pf1N1B4_1908 0.75 -0.7 2,183,424 + Pf1N1B4_1908 0.79 -0.5 2,183,424 + Pf1N1B4_1908 0.79 -2.4 2,183,424 + Pf1N1B4_1908 0.79 -0.3 2,183,424 + Pf1N1B4_1908 0.79 +0.6 2,183,424 + +3.9 2,183,425 - Pf1N1B4_1908 0.80 -1.3 2,183,451 + Pf1N1B4_1908 0.82 +1.8 2,183,451 + Pf1N1B4_1908 0.82 +0.0
Or see this region's nucleotide sequence