Experiment: Rifampicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1611 and Pf1N1B4_1612 are separated by 630 nucleotides Pf1N1B4_1612 and Pf1N1B4_1613 overlap by 2 nucleotides Pf1N1B4_1613 and Pf1N1B4_1614 are separated by 205 nucleotides
Pf1N1B4_1611 - Nitrogen regulation protein NR(I), at 1,855,823 to 1,857,259
_1611
Pf1N1B4_1612 - FIG00955429: hypothetical protein, at 1,857,890 to 1,858,330
_1612
Pf1N1B4_1613 - tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207), at 1,858,329 to 1,858,784
_1613
Pf1N1B4_1614 - Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state), at 1,858,990 to 1,859,475
_1614
Position (kb)
1857
1858
1859 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1857.184 kb on + strand at 1857.184 kb on + strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.185 kb on - strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.257 kb on + strand at 1857.258 kb on - strand at 1857.262 kb on - strand at 1857.626 kb on + strand at 1857.627 kb on - strand at 1857.627 kb on - strand at 1857.653 kb on + strand at 1857.653 kb on + strand at 1857.653 kb on + strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.654 kb on - strand at 1857.708 kb on + strand at 1857.708 kb on + strand at 1857.734 kb on + strand at 1857.734 kb on + strand at 1857.734 kb on + strand at 1857.812 kb on + strand at 1857.812 kb on + strand at 1857.812 kb on + strand at 1857.813 kb on - strand at 1857.813 kb on - strand at 1857.813 kb on - strand at 1857.864 kb on + strand at 1857.865 kb on - strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.891 kb on + strand at 1857.892 kb on - strand at 1857.892 kb on - strand at 1857.892 kb on - strand at 1858.079 kb on + strand, within Pf1N1B4_1612 at 1858.080 kb on - strand, within Pf1N1B4_1612 at 1858.080 kb on - strand, within Pf1N1B4_1612 at 1858.265 kb on + strand, within Pf1N1B4_1612 at 1858.265 kb on + strand, within Pf1N1B4_1612 at 1858.265 kb on + strand, within Pf1N1B4_1612 at 1858.294 kb on + strand at 1858.312 kb on + strand at 1858.312 kb on + strand at 1858.313 kb on - strand at 1858.313 kb on - strand at 1858.489 kb on - strand, within Pf1N1B4_1613 at 1858.489 kb on - strand, within Pf1N1B4_1613 at 1858.489 kb on - strand, within Pf1N1B4_1613 at 1858.563 kb on - strand, within Pf1N1B4_1613 at 1858.752 kb on + strand at 1858.783 kb on - strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.025 kb on + strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.026 kb on - strand at 1859.045 kb on + strand, within Pf1N1B4_1614 at 1859.045 kb on + strand, within Pf1N1B4_1614 at 1859.046 kb on - strand, within Pf1N1B4_1614 at 1859.046 kb on - strand, within Pf1N1B4_1614 at 1859.046 kb on - strand, within Pf1N1B4_1614
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml remove 1,857,184 + -0.9 1,857,184 + +1.3 1,857,185 - +0.0 1,857,185 - -0.3 1,857,185 - +1.0 1,857,185 - -0.3 1,857,185 - -0.3 1,857,257 + +0.6 1,857,257 + +0.9 1,857,257 + -1.3 1,857,257 + +0.1 1,857,257 + +2.0 1,857,257 + -0.1 1,857,258 - -0.3 1,857,262 - +2.5 1,857,626 + -0.2 1,857,627 - -1.1 1,857,627 - -2.3 1,857,653 + +1.5 1,857,653 + +0.5 1,857,653 + -0.9 1,857,654 - -1.8 1,857,654 - +0.5 1,857,654 - +0.6 1,857,654 - -1.1 1,857,654 - -1.3 1,857,708 + +1.2 1,857,708 + -1.8 1,857,734 + -0.3 1,857,734 + -0.3 1,857,734 + -1.3 1,857,812 + -0.8 1,857,812 + -1.1 1,857,812 + +0.3 1,857,813 - +0.8 1,857,813 - +0.0 1,857,813 - +1.2 1,857,864 + -0.7 1,857,865 - +0.2 1,857,891 + -0.8 1,857,891 + -0.7 1,857,891 + -1.0 1,857,891 + +0.5 1,857,891 + -0.2 1,857,891 + -0.2 1,857,891 + -0.6 1,857,892 - +1.7 1,857,892 - +1.2 1,857,892 - -0.7 1,858,079 + Pf1N1B4_1612 0.43 -0.8 1,858,080 - Pf1N1B4_1612 0.43 -1.6 1,858,080 - Pf1N1B4_1612 0.43 +0.4 1,858,265 + Pf1N1B4_1612 0.85 -1.6 1,858,265 + Pf1N1B4_1612 0.85 -1.0 1,858,265 + Pf1N1B4_1612 0.85 +0.8 1,858,294 + -0.1 1,858,312 + -2.7 1,858,312 + -1.6 1,858,313 - +0.7 1,858,313 - +1.5 1,858,489 - Pf1N1B4_1613 0.35 +0.6 1,858,489 - Pf1N1B4_1613 0.35 -1.6 1,858,489 - Pf1N1B4_1613 0.35 +0.9 1,858,563 - Pf1N1B4_1613 0.51 -0.0 1,858,752 + -1.0 1,858,783 - -1.1 1,859,025 + +0.2 1,859,025 + -0.3 1,859,025 + +1.2 1,859,025 + +0.2 1,859,025 + -0.3 1,859,026 - -0.3 1,859,026 - -1.5 1,859,026 - -0.8 1,859,026 - -1.6 1,859,026 - -0.2 1,859,026 - -0.9 1,859,026 - -0.1 1,859,026 - +1.2 1,859,026 - -1.1 1,859,026 - -0.2 1,859,026 - +2.5 1,859,026 - -0.8 1,859,045 + Pf1N1B4_1614 0.11 -0.8 1,859,045 + Pf1N1B4_1614 0.11 +1.5 1,859,046 - Pf1N1B4_1614 0.12 -1.6 1,859,046 - Pf1N1B4_1614 0.12 -1.3 1,859,046 - Pf1N1B4_1614 0.12 -0.3
Or see this region's nucleotide sequence