Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_149

Experiment: Rifampicin 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_148 and Pf1N1B4_149 overlap by 4 nucleotidesPf1N1B4_149 and Pf1N1B4_150 are separated by 304 nucleotides Pf1N1B4_148 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 182,592 to 188,378 _148 Pf1N1B4_149 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD, at 188,375 to 191,797 _149 Pf1N1B4_150 - SyrP-like protein, at 192,102 to 193,085 _150 Position (kb) 188 189 190 191 192Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 187.720 kb on + strand, within Pf1N1B4_148at 188.378 kb on + strandat 188.379 kb on - strandat 188.379 kb on - strandat 188.691 kb on - strandat 188.691 kb on - strandat 188.886 kb on + strand, within Pf1N1B4_149at 189.033 kb on + strand, within Pf1N1B4_149at 189.180 kb on + strand, within Pf1N1B4_149at 189.180 kb on + strand, within Pf1N1B4_149at 189.180 kb on + strandat 189.530 kb on - strand, within Pf1N1B4_149at 189.783 kb on + strand, within Pf1N1B4_149at 189.783 kb on + strand, within Pf1N1B4_149at 189.784 kb on - strand, within Pf1N1B4_149at 189.813 kb on - strand, within Pf1N1B4_149at 190.002 kb on + strand, within Pf1N1B4_149at 190.002 kb on + strand, within Pf1N1B4_149at 190.003 kb on - strand, within Pf1N1B4_149at 190.003 kb on - strand, within Pf1N1B4_149at 190.150 kb on - strand, within Pf1N1B4_149at 190.413 kb on + strand, within Pf1N1B4_149at 190.413 kb on + strand, within Pf1N1B4_149at 191.193 kb on + strand, within Pf1N1B4_149at 191.193 kb on + strand, within Pf1N1B4_149at 191.194 kb on - strand, within Pf1N1B4_149at 191.194 kb on - strand, within Pf1N1B4_149at 191.584 kb on - strandat 191.584 kb on - strandat 191.637 kb on + strandat 191.637 kb on + strandat 191.637 kb on + strandat 191.638 kb on - strandat 191.883 kb on + strandat 191.906 kb on - strandat 192.305 kb on - strand, within Pf1N1B4_150at 192.598 kb on + strandat 192.653 kb on - strand, within Pf1N1B4_150at 192.653 kb on - strand, within Pf1N1B4_150at 192.712 kb on + strand, within Pf1N1B4_150at 192.713 kb on - strand, within Pf1N1B4_150at 192.746 kb on - strand, within Pf1N1B4_150at 192.746 kb on - strand, within Pf1N1B4_150at 192.746 kb on - strand, within Pf1N1B4_150

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Per-strain Table

Position Strand Gene LocusTag Fraction Rifampicin 0.004 mg/ml
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187,720 + Pf1N1B4_148 0.89 -0.9
188,378 + -0.7
188,379 - -0.9
188,379 - -0.4
188,691 - -1.7
188,691 - -1.0
188,886 + Pf1N1B4_149 0.15 -1.5
189,033 + Pf1N1B4_149 0.19 +0.8
189,180 + Pf1N1B4_149 0.24 +0.3
189,180 + Pf1N1B4_149 0.24 -2.3
189,180 + +0.8
189,530 - Pf1N1B4_149 0.34 +0.3
189,783 + Pf1N1B4_149 0.41 -0.5
189,783 + Pf1N1B4_149 0.41 +0.8
189,784 - Pf1N1B4_149 0.41 +0.5
189,813 - Pf1N1B4_149 0.42 -0.5
190,002 + Pf1N1B4_149 0.48 -0.7
190,002 + Pf1N1B4_149 0.48 +0.8
190,003 - Pf1N1B4_149 0.48 +0.1
190,003 - Pf1N1B4_149 0.48 -0.7
190,150 - Pf1N1B4_149 0.52 -1.2
190,413 + Pf1N1B4_149 0.60 +0.8
190,413 + Pf1N1B4_149 0.60 +0.5
191,193 + Pf1N1B4_149 0.82 +0.6
191,193 + Pf1N1B4_149 0.82 +2.8
191,194 - Pf1N1B4_149 0.82 +1.3
191,194 - Pf1N1B4_149 0.82 +0.3
191,584 - -0.9
191,584 - +0.8
191,637 + +0.5
191,637 + +1.2
191,637 + +0.3
191,638 - +1.0
191,883 + +0.5
191,906 - -0.2
192,305 - Pf1N1B4_150 0.21 -1.7
192,598 + -0.7
192,653 - Pf1N1B4_150 0.56 +1.4
192,653 - Pf1N1B4_150 0.56 -0.2
192,712 + Pf1N1B4_150 0.62 +0.8
192,713 - Pf1N1B4_150 0.62 -2.9
192,746 - Pf1N1B4_150 0.65 +0.7
192,746 - Pf1N1B4_150 0.65 +1.0
192,746 - Pf1N1B4_150 0.65 +1.8

Or see this region's nucleotide sequence